Protocol abstract

Nature Protocols 4, 44 - 57 (2009)
Published online: 18 December 2008 | doi:10.1038/nprot.2008.211

Subject Category: Computational and theoretical biology

Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

Da Wei Huang1,2, Brad T Sherman1,2 & Richard A Lempicki1

DAVID bioinformatics resources consists of an integrated biological knowledgebase and analytic tools aimed at systematically extracting biological meaning from large gene/protein lists. This protocol explains how to use DAVID, a high-throughput and integrated data-mining environment, to analyze gene lists derived from high-throughput genomic experiments. The procedure first requires uploading a gene list containing any number of common gene identifiers followed by analysis using one or more text and pathway-mining tools such as gene functional classification, functional annotation chart or clustering and functional annotation table. By following this protocol, investigators are able to gain an in-depth understanding of the biological themes in lists of genes that are enriched in genome-scale studies.

  1. Laboratory of Immunopathogenesis and Bioinformatics, Clinical Services Program, SAIC-Frederick Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702, USA.
  2. These authors contributed equally to this work.

Correspondence to: Richard A Lempicki1 e-mail:

Correspondence to: Da Wei Huang1,2 e-mail:


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