Latest Protocols
Volume 3 No 9
- Archive
In order to view all protocols, including both Nature Protocols and Protocols Network protocols, please browse by category.
To facilitate archiving and citing, Nature Protocols are allocated to publication months and assigned unique page numbers.
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Nature protocols
Cardiac commitment of primate embryonic stem cells - pp1381 - 1387
Julia Leschik, Sonia Stefanovic, Benjamin Brinon & Michel Pucéat
Published online: 07 August 2008 | doi:10.1038/nprot.2008.116
Abstract - Cardiac commitment of primate embryonic stem cells | Full Text - Cardiac commitment of primate embryonic stem cells | PDF (287 KB) - Cardiac commitment of primate embryonic stem cells
A highly sensitive single-enzyme homocysteine assay - pp1388 - 1394
Yuying Tan & Robert M Hoffman
Published online: 07 August 2008 | doi:10.1038/nprot.2008.117
Abstract - A highly sensitive single-enzyme homocysteine assay | Full Text - A highly sensitive single-enzyme homocysteine assay | PDF (187 KB) - A highly sensitive single-enzyme homocysteine assay | Supplementary information
Semiautomated and rapid quantification of nucleic acid footprinting and structure mapping experiments - pp1395 - 1401
Alain Laederach, Rhiju Das, Quentin Vicens, Samuel M Pearlman, Michael Brenowitz, Daniel Herschlag & Russ B Altman
Published online: 07 August 2008 | doi:10.1038/nprot.2008.134
Abstract - Semiautomated and rapid quantification of nucleic acid footprinting and structure mapping experiments | Full Text - Semiautomated and rapid quantification of nucleic acid footprinting and structure mapping experiments | PDF (733 KB) - Semiautomated and rapid quantification of nucleic acid footprinting and structure mapping experiments | Supplementary information
Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells - pp1402 - 1413
Hanyi Zhuang & Hiroaki Matsunami
Published online: 14 August 2008 | doi:10.1038/nprot.2008.120
Abstract - Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells | Full Text - Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells | PDF (300 KB) - Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells
PCR-based gene targeting in Candida albicans - pp1414 - 1421
Andrea Walther & Jürgen Wendland
Published online: 14 August 2008 | doi:10.1038/nprot.2008.137
Abstract - PCR-based gene targeting in : Candida albicans | Full Text - PCR-based gene targeting in Candida albicans | PDF (257 KB) - PCR-based gene targeting in Candida albicans | Supplementary information
Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats - pp1422 - 1434
Pál Pacher, Takahiro Nagayama, Partha Mukhopadhyay, Sándor Bátkai & David A Kass
Published online: 14 August 2008 | doi:10.1038/nprot.2008.138
Abstract - Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats | Full Text - Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats | PDF (1,467 KB) - Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats | Supplementary information
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification - pp1435 - 1443
Stefan R Braam, Chris Denning, Elena Matsa, Lorraine E Young, Robert Passier & Christine L Mummery
Published online: 14 August 2008 | doi:10.1038/nprot.2008.140
Abstract - Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification | Full Text - Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification | PDF (346 KB) - Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification
Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data - pp1444 - 1451
Christine Vogel & Edward M Marcotte
Published online: 21 August 2008 | doi:10.1038/nport.2008.132
Abstract - Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data | Full Text - Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data | PDF (417 KB) - Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data | Supplementary information
Touchdown PCR for increased specificity and sensitivity in PCR amplification - pp1452 - 1456
Darren J Korbie & John S Mattick
Published online: 21 August 2008 | doi:10.1038/nprot.2008.133
Abstract - Touchdown PCR for increased specificity and sensitivity in PCR amplification | Full Text - Touchdown PCR for increased specificity and sensitivity in PCR amplification | PDF (151 KB) - Touchdown PCR for increased specificity and sensitivity in PCR amplification
The preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments - pp1457 - 1466
Kathrin Lang & Ronald Micura
Published online: 21 August 2008 | doi:10.1038/nprot.2008.135
Abstract - The preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments | Full Text - The preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments | PDF (412 KB) - The preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments
Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry - pp1467 - 1475
Huanwen Chen & Renato Zenobi
Published online: 28 August 2008 | doi:10.1038/nprot.2008.109
Abstract - Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry | Full Text - Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry | PDF (623 KB) - Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry
High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry - pp1476 - 1484
Sun Pyo Hong, Soo-Kyung Shin, Eun Hee Lee, Eun Ok Kim, Seung Il Ji, Hyun Jae Chung, Sun Nie Park, Wangdon Yoo, William R Folk & Soo-Ok Kim
Published online: 28 August 2008 | doi:10.1038/nprot.2008.136
Abstract - High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry | Full Text - High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry | PDF (261 KB) - High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry | Supplementary information
Peptide microarrays for high-throughput studies of Ser/Thr phosphatases - pp1485 - 1493
Hongyan Sun, Candy H S Lu, Haibin Shi, Liqian Gao & Shao Q Yao
Published online: 28 August 2008 | doi:10.1038/nprot.2008.139
Abstract - Peptide microarrays for high-throughput studies of Ser/Thr phosphatases | Full Text - Peptide microarrays for high-throughput studies of Ser/Thr phosphatases | PDF (426 KB) - Peptide microarrays for high-throughput studies of Ser/Thr phosphatases
A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing - pp1494 - 1500
Christopher G Pierce, Priya Uppuluri, Amanda R Tristan, Floyd L Wormley, Jr, Eilidh Mowat, Gordon Ramage & Jose L Lopez-Ribot
Published online: 28 August 2008 | doi:10.1038/nport.2008.141
Abstract - A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing | Full Text - A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing | PDF (181 KB) - A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing
Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique - pp1501 - 1509
Burhan Gharaibeh, Aiping Lu, Jessica Tebbets, Bo Zheng, Joe Feduska, Mihaela Crisan, Bruno Péault, James Cummins & Johnny Huard
Published online: 04 September 2008 | doi:10.1038/nprot.2008.142
Abstract - Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique | Full Text - Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique | PDF (367 KB) - Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique
Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events - pp1510 - 1526
Kurt Pfannkuche, Frank Thomas Wunderlich, Michael Xavier Doss, Dimitry Spitkovsky, Michael Reppel, Agapios Sachinidis & Jürgen Hescheler
Published online: 04 September 2008 | doi:10.1038/nprot.2008.143
Abstract - Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events | Full Text - Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events | PDF (546 KB) - Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events | Supplementary information
Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins - pp1527 - 1534
Stephen R Adams & Roger Y Tsien
Published online: 04 September 2008 | doi:10.1038/nprot.2008.144
Abstract - Preparation of the membrane-permeant biarsenicals FlAsH-EDT: 2: and ReAsH-EDT: 2: for fluorescent labeling of tetracysteine-tagged proteins | Full Text - Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins | PDF (236 KB) - Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins
