A three-component Fischer indole synthesis - pp1249 - 1252

Christopher A Simoneau & Bruce Ganem

Published online: 17 July 2008 | doi:10.1038/nprot.2008.94

Abstract - A three-component Fischer indole synthesis | Full Text - A three-component Fischer indole synthesis | PDF (204 KB) - A three-component Fischer indole synthesis

Subject Category: Synthetic chemistry

Primary culture and phenotyping of murine chondrocytes - pp1253 - 1260

Marjolaine Gosset, Francis Berenbaum, Sylvie Thirion & Claire Jacques

Published online: 17 July 2008 | doi:10.1038/nprot.2008.95

Abstract - Primary culture and phenotyping of murine chondrocytes | Full Text - Primary culture and phenotyping of murine chondrocytes | PDF (409 KB) - Primary culture and phenotyping of murine chondrocytes

Subject Categories: Cell and tissue culture | Model organisms

Cultivating previously uncultured soil bacteria using a soil substrate membrane system - pp1261 - 1269

Belinda C Ferrari, Tristrom Winsley, Michael Gillings & Svend Binnerup

Published online: 17 July 2008 | doi:10.1038/nprot.2008.102

Abstract - Cultivating previously uncultured soil bacteria using a soil substrate membrane system | Full Text - Cultivating previously uncultured soil bacteria using a soil substrate membrane system | PDF (297 KB) - Cultivating previously uncultured soil bacteria using a soil substrate membrane system

Subject Categories: Cell and tissue culture | Microbiology and virology

Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test - pp1270 - 1277

Viviana S Fluxá, Denis Wahler & Jean-Louis Reymond

Published online: 17 July 2008 | doi:10.1038/nprot.2008.106

Abstract - Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test | Full Text - Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test | PDF (333 KB) - Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test

Subject Category: Biochemistry and protein analysis

Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye - pp1278 - 1286

Stephan Speier, Daniel Nyqvist, Martin Köhler, Alejandro Caicedo, Ingo B Leibiger & Per-Olof Berggren

Published online: 17 July 2008 | doi:10.1038/nprot.2008.118

Abstract - Noninvasive high-resolution : in vivo: imaging of cell biology in the anterior chamber of the mouse eye | Full Text - Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye | PDF (693 KB) - Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye

Subject Categories: Cell and developmental biology | Imaging | Model organisms

Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry - pp1287 - 1298

Koli Taghizadeh, Jose L McFaline, Bo Pang, Matthew Sullivan, Min Dong, Elaine Plummer & Peter C Dedon

Published online: 17 July 2008 | doi:10.1038/nprot.2008.119

Abstract - Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry | Full Text - Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry | PDF (400 KB) - Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry

Subject Categories: Genetic analysis | Spectroscopy and structural analysis

Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach - pp1299 - 1311

Bryson D Bennett, Jie Yuan, Elizabeth H Kimball & Joshua D Rabinowitz

Published online: 17 July 2008 | doi:10.1038/nprot.2008.107

Abstract - Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach | Full Text - Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach | PDF (519 KB) - Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach

Subject Categories: Biochemistry and protein analysis | Genomics and proteomics | Spectroscopy and structural analysis

See also: Protocol by Yuan et al.

PCR-amplification of GC-rich regions: 'slowdown PCR' - pp1312 - 1317

Ulrich H Frey, Hagen S Bachmann, Jürgen Peters & Winfried Siffert

Published online: 24 July 2008 | doi:10.1038/nprot.2008.112

Abstract - PCR-amplification of GC-rich regions: 'slowdown PCR' | Full Text - PCR-amplification of GC-rich regions: 'slowdown PCR' | PDF (262 KB) - PCR-amplification of GC-rich regions: 'slowdown PCR'

Subject Category: Nucleic acid based molecular biology

Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos - pp1318 - 1327

Kin-Mei Leung & Christine E Holt

Published online: 24 July 2008 | doi:10.1038/nprot.2008.113

Abstract - Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos | Full Text - Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos | PDF (382 KB) - Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos

Subject Categories: Cell and tissue culture | Genetic analysis | Imaging | Model organisms

Kinetic flux profiling for quantitation of cellular metabolic fluxes - pp1328 - 1340

Jie Yuan, Bryson D Bennett & Joshua D Rabinowitz

Published online: 24 July 2008 | doi:10.1038/nprot.2008.131

Abstract - Kinetic flux profiling for quantitation of cellular metabolic fluxes | Full Text - Kinetic flux profiling for quantitation of cellular metabolic fluxes | PDF (1,114 KB) - Kinetic flux profiling for quantitation of cellular metabolic fluxes

Subject Categories: Biochemistry and protein analysis | Genomics and proteomics

See also: Protocol by Bennett et al.

Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis - pp1341 - 1349

Hin-Koon Woo, Trent R Northen, Oscar Yanes & Gary Siuzdak

Published online: 31 July 2008 | doi:10.1038/nprot.2008.110

Abstract - Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis | Full Text - Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis | PDF (869 KB) - Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis

Subject Categories: Nanotechnology | Spectroscopy and structural analysis

Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries - pp1350 - 1363

Elizabeth R Sharlow, Stephanie Leimgruber, Archibong Yellow-Duke, Rebecca Barrett, Qiming Jane Wang & John S Lazo

Published online: 31 July 2008 | doi:10.1038/nprot.2008.111

Abstract - Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries | Full Text - Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries | PDF (300 KB) - Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries

Subject Categories: Biochemistry and protein analysis | Isolation, purification and separation | Pharmacology and toxicology

The fluorometric microculture cytotoxicity assay - pp1364 - 1369

Elin Lindhagen, Peter Nygren & Rolf Larsson

Published online: 31 July 2008 | doi:10.1038/nprot.2008.114

Abstract - The fluorometric microculture cytotoxicity assay | Full Text - The fluorometric microculture cytotoxicity assay | PDF (176 KB) - The fluorometric microculture cytotoxicity assay

Subject Categories: Biochemistry and protein analysis | Pharmacology and toxicology

Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization - pp1370 - 1379

Raphael Pinaud, Claudio V Mello, Tarciso A Velho, Ryan D Wynne & Liisa A Tremere

Published online: 31 July 2008 | doi:10.1038/nprot.2008.115

Abstract - Detection of two mRNA species at single-cell resolution by double-fluorescence : in situ: hybridization | Full Text - Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization | PDF (467 KB) - Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization

Subject Categories: Genetic analysis | Imaging | Neuroscience