Access
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
Protocol
Nature Protocols 3, 1194–1201 (1 June 2008) | doi:10.1038/nprot.2008.103
An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies
&
Abstract
Determining the precise expression pattern of a gene of interest at various stages of development is essential to understanding its biological function in embryology. This protocol describes a sensitive method for whole-mount in situ hybridization (WISH) to mouse embryos, using cRNA probes. Adaptations are provided that allow the protocol to be applied to embryonic stages ranging from blastocysts to postimplantation stage embryos, and to embryoid bodies. We also describe an in situ method for differential detection of two probes. Probe labeling and dissection and preparation of the embryos can be performed in 2 d. The actual WISH procedure can be completed in another 3 d.
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
