Latest Protocols
Volume 3 No 7
- Archive
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Nature protocols
Purification of matrix metalloproteinases by column chromatography - pp1111 - 1124
Kazushi Imai & Yasunori Okada
Published online: 12 June 2008 | doi:10.1038/nprot.2008.74
Abstract - Purification of matrix metalloproteinases by column chromatography | Full Text - Purification of matrix metalloproteinases by column chromatography | PDF (405 KB) - Purification of matrix metalloproteinases by column chromatography
Neutral red uptake assay for the estimation of cell viability/cytotoxicity - pp1125 - 1131
Guillermo Repetto, Ana del Peso & Jorge L Zurita
Published online: 12 June 2008 | doi:10.1038/nprot.2008.75
Abstract - Neutral red uptake assay for the estimation of cell viability/cytotoxicity | Full Text - Neutral red uptake assay for the estimation of cell viability/cytotoxicity | PDF (308 KB) - Neutral red uptake assay for the estimation of cell viability/cytotoxicity
Atomic force microscopy and chemical force microscopy of microbial cells - pp1132 - 1138
Yves F Dufrêne
Published online: 12 June 2008 | doi:10.1038/nprot.2008.101
Abstract - Atomic force microscopy and chemical force microscopy of microbial cells | Full Text - Atomic force microscopy and chemical force microscopy of microbial cells | PDF (395 KB) - Atomic force microscopy and chemical force microscopy of microbial cells
Ion mobility–mass spectrometry analysis of large protein complexes - pp1139 - 1152
Brandon T Ruotolo, Justin L P Benesch, Alan M Sandercock, Suk-Joon Hyung & Carol V Robinson
Published online: 19 June 2008 | doi:10.1038/nprot.2008.78
Abstract - Ion mobility-mass spectrometry analysis of large protein complexes | Full Text - Ion mobility–mass spectrometry analysis of large protein complexes | PDF (552 KB) - Ion mobility–mass spectrometry analysis of large protein complexes
Analysis of mutational spectra by denaturing capillary electrophoresis - pp1153 - 1166
Per O Ekstrøm, Konstantin Khrapko, Xiao-Cheng Li-Sucholeiki, Ian W Hunter & William G Thilly
Published online: 19 June 2008 | doi:10.1038/nprot.2008.79
Abstract - Analysis of mutational spectra by denaturing capillary electrophoresis | Full Text - Analysis of mutational spectra by denaturing capillary electrophoresis | PDF (530 KB) - Analysis of mutational spectra by denaturing capillary electrophoresis
A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice - pp1167 - 1170
Claudia Mitchell & Holger Willenbring
Published online: 19 June 2008 | doi:10.1038/nprot.2008.80
Abstract - A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice | Full Text - A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice | PDF (254 KB) - A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice | Supplementary information
Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 - pp1171 - 1179
Gerrit Langer, Serge X Cohen, Victor S Lamzin & Anastassis Perrakis
Published online: 19 June 2008 | doi:10.1038/nprot.2008.91
Abstract - Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 | Full Text - Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 | PDF (342 KB) - Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7
Generation of human-induced pluripotent stem cells - pp1180 - 1186
In-Hyun Park, Paul H Lerou, Rui Zhao, Hongguang Huo & George Q Daley
Published online: 26 June 2008 | doi:10.1038/nprot.2008.92
Abstract - Generation of human-induced pluripotent stem cells | Full Text - Generation of human-induced pluripotent stem cells | PDF (282 KB) - Generation of human-induced pluripotent stem cells | Supplementary information
An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells - pp1187 - 1193
Aida Muslimovic, Ismail Hassan Ismail, Yue Gao & Ola Hammarsten
Published online: 26 June 2008 | doi:10.1038/nprot.2008.93
Abstract - An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells | Full Text - An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells | PDF (226 KB) - An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells
An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies - pp1194 - 1201
Denis Piette, Marijke Hendrickx, Erik Willems, Caroline R Kemp & Luc Leyns
Published online: 26 June 2008 | doi:10.1038/nprot.2008.103
Abstract - An optimized procedure for whole-mount : in situ: hybridization on mouse embryos and embryoid bodies | Full Text - An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies | PDF (293 KB) - An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies
Substrate-induced gene expression (SIGEX) screening of metagenome libraries - pp1202 - 1212
Taku Uchiyama & Kazuya Watanabe
Published online: 03 July 2008 | doi:10.1038/nprot.2008.96
Abstract - Substrate-induced gene expression (SIGEX) screening of metagenome libraries | Full Text - Substrate-induced gene expression (SIGEX) screening of metagenome libraries | PDF (324 KB) - Substrate-induced gene expression (SIGEX) screening of metagenome libraries
3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures - pp1213 - 1227
Xiang-Jun Lu & Wilma K Olson
Published online: 03 July 2008 | doi:10.1038/nprot.2008.104
Abstract - 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures | Full Text - 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures | PDF (720 KB) - 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures | Supplementary information
Isolation of mitochondria from rat brain using Percoll density gradient centrifugation - pp1228 - 1239
Neil R Sims & Michelle F Anderson
Published online: 03 July 2008 | doi:10.1038/nprot.2008.105
Abstract - Isolation of mitochondria from rat brain using Percoll density gradient centrifugation | Full Text - Isolation of mitochondria from rat brain using Percoll density gradient centrifugation | PDF (259 KB) - Isolation of mitochondria from rat brain using Percoll density gradient centrifugation
A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load - pp1240 - 1248
Mauro S Malnati, Gabriella Scarlatti, Francesca Gatto, Francesca Salvatori, Giulia Cassina, Teresa Rutigliano, Rosy Volpi & Paolo Lusso
Published online: 03 July 2008 | doi:10.1038/nprot.2008.108
Abstract - A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load | Full Text - A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load | PDF (246 KB) - A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load
