Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells - pp935 - 940

Andrew Chojnacki & Samuel Weiss

Published online: 08 May 2008 | doi:10.1038/nprot.2008.55

Abstract - Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells | Full Text - Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells | PDF (219 KB) - Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells

The selective detection of mitochondrial superoxide by live cell imaging - pp941 - 947

Kristine M Robinson, Michael S Janes & Joseph S Beckman

Published online: 08 May 2008 | doi:10.1038/nprot.2008.56

Abstract - The selective detection of mitochondrial superoxide by live cell imaging | Full Text - The selective detection of mitochondrial superoxide by live cell imaging | PDF (268 KB) - The selective detection of mitochondrial superoxide by live cell imaging

Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies - pp948 - 954

Silvia Curado, Didier Y R Stainier & Ryan M Anderson

Published online: 08 May 2008 | doi:10.1038/nprot.2008.58

Abstract - Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies | Full Text - Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies | PDF (324 KB) - Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies

Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry - pp955 - 964

Hiep Phuc Dong, Lilach Kleinberg, Ben Davidson & Björn Risberg

Published online: 08 May 2008 | doi:10.1038/nprot.2008.77

Abstract - Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry | Full Text - Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry | PDF (577 KB) - Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry

Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells - pp965 - 976

Andrey V Kuznetsov, Vladimir Veksler, Frank N Gellerich, Valdur Saks, Raimund Margreiter & Wolfram S Kunz

Published online: 15 May 2008 | doi:10.1038/nprot.2008.61

Abstract - Analysis of mitochondrial function : in situ: in permeabilized muscle fibers, tissues and cells | Full Text - Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells | PDF (498 KB) - Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells

Image processing for electron microscopy single-particle analysis using XMIPP - pp977 - 990

Sjors H W Scheres, Rafael Núñez-Ramírez, Carlos O S Sorzano, José María Carazo & Roberto Marabini

Published online: 15 May 2008 | doi:10.1038/nprot.2008.62

Abstract - Image processing for electron microscopy single-particle analysis using XMIPP | Full Text - Image processing for electron microscopy single-particle analysis using XMIPP | PDF (608 KB) - Image processing for electron microscopy single-particle analysis using XMIPP

Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates - pp991 - 1000

Brice Korkmaz, Sylvie Attucci, Maria Aparecida Juliano, Timofey Kalupov, Marie-Lise Jourdan, Luiz Juliano & Francis Gauthier

Published online: 15 May 2008 | doi:10.1038/nprot.2008.63

Abstract - Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates | Full Text - Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates | PDF (474 KB) - Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates

¹H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues - pp1001 - 1012

Nicholas J Kruger, M Adrian Troncoso-Ponce & R George Ratcliffe

Published online: 22 May 2008 | doi:10.1038/nprot.2008.64

Abstract - 1: H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues | Full Text - 1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues | PDF (359 KB) - 1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues

Optimized mouse ES cell culture system by suspension growth in a fully defined medium - pp1013 - 1017

Michael Andäng, Annalena Moliner, Claudia A Doege, Carlos F Ibañez & Patrik Ernfors

Published online: 22 May 2008 | doi:10.1038/nprot.2008.65

Abstract - Optimized mouse ES cell culture system by suspension growth in a fully defined medium | Full Text - Optimized mouse ES cell culture system by suspension growth in a fully defined medium | PDF (211 KB) - Optimized mouse ES cell culture system by suspension growth in a fully defined medium

An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants - pp1018 - 1025

Abdelaty Saleh, Raúl Alvarez-Venegas & Zoya Avramova

Published online: 22 May 2008 | doi:10.1038/nprot.2008.66

Abstract - An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in : Arabidopsis: plants | Full Text - An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants | PDF (245 KB) - An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants

See also: Protocol by Dahl & Collas

Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast - pp1026 - 1031

Terrance A Stadheim, Huijuan Li, Warren Kett, Irina N Burnina & Tillman U Gerngross

Published online: 22 May 2008 | doi:10.1038/nprot.2008.76

Abstract - Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast | Full Text - Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast | PDF (214 KB) - Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast

A rapid micro chromatin immunoprecipitation assay (ChIP) - pp1032 - 1045

John Arne Dahl & Philippe Collas

Published online: 29 May 2008 | doi:10.1038/nprot.2008.68

Abstract - A rapid micro chromatin immunoprecipitation assay (ChIP) | Full Text - A rapid micro chromatin immunoprecipitation assay (ChIP) | PDF (523 KB) - A rapid micro chromatin immunoprecipitation assay (ChIP) | Supplementary information

See also: Protocol by Saleh et al.

Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro - pp1046 - 1055

Colin McGuckin, Marcin Jurga, Hamad Ali, Marko Strbad & Nicolas Forraz

Published online: 29 May 2008 | doi:10.1038/nprot.2008.69

Abstract - Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells : in vitro | Full Text - Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro | PDF (503 KB) - Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro

A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond - pp1056 - 1076

Sen Wu, Guoxin Ying, Qiang Wu & Mario R Capecchi

Published online: 29 May 2008 | doi:10.1038/nprot.2008.70

Abstract - A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond | Full Text - A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond | PDF (914 KB) - A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond | Supplementary information

Improved northern blot method for enhanced detection of small RNA - pp1077 - 1084

Gurman S Pall & Andrew J Hamilton

Published online: 05 June 2008 | doi:10.1038/nprot.2008.67

Abstract - Improved northern blot method for enhanced detection of small RNA | Full Text - Improved northern blot method for enhanced detection of small RNA | PDF (291 KB) - Improved northern blot method for enhanced detection of small RNA | Supplementary information

Isolation of endothelial cells from fresh tissues - pp1085 - 1091

Judy R van Beijnum, Mat Rousch, Karolien Castermans, Edith van der Linden & Arjan W Griffioen

Published online: 05 June 2008 | doi:10.1038/nprot.2008.71

Abstract - Isolation of endothelial cells from fresh tissues | Full Text - Isolation of endothelial cells from fresh tissues | PDF (551 KB) - Isolation of endothelial cells from fresh tissues

Footprinting protein–DNA complexes using the hydroxyl radical - pp1092 - 1100

Swapan S Jain & Thomas D Tullius

Published online: 05 June 2008 | doi:10.1038/nprot.2008.72

Abstract - Footprinting protein-DNA complexes using the hydroxyl radical | Full Text - Footprinting protein–DNA complexes using the hydroxyl radical | PDF (270 KB) - Footprinting protein–DNA complexes using the hydroxyl radical

Analyzing real-time PCR data by the comparative C_T method - pp1101 - 1108

Thomas D Schmittgen & Kenneth J Livak

Published online: 05 June 2008 | doi:10.1038/nprot.2008.73

Abstract - Analyzing real-time PCR data by the comparative : C: T: method | Full Text - Analyzing real-time PCR data by the comparative CT method | PDF (471 KB) - Analyzing real-time PCR data by the comparative CT method