Preparation of magnetic resonance contrast agents activated by -galactosidase - pp341 - 350

Lauren M Urbanczyk-Pearson & Thomas J Meade

Published online: 14 February 2008 | doi:10.1038/nprot.2007.529

Abstract - Preparation of magnetic resonance contrast agents activated by [beta]-galactosidase | Full Text - Preparation of magnetic resonance contrast agents activated by -galactosidase | PDF (225 KB) - Preparation of magnetic resonance contrast agents activated by -galactosidase | Supplementary information

An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications - pp351 - 356

Samit Shah & Simon H Friedman

Published online: 14 February 2008 | doi:10.1038/nprot.2007.535

Abstract - An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications | Full Text - An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications | PDF (296 KB) - An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications

A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution - pp357 - 362

Gavin J Williams & Jon S Thorson

Published online: 14 February 2008 | doi:10.1038/nprot.2007.538

Abstract - A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution | Full Text - A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution | PDF (178 KB) - A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution

Isolation and propagation of HIV-1 on peripheral blood mononuclear cells - pp363 - 370

Angélique B van 't Wout, Hanneke Schuitemaker & Neeltje A Kootstra

Published online: 14 February 2008 | doi:10.1038/nprot.2008.3

Abstract - Isolation and propagation of HIV-1 on peripheral blood mononuclear cells | Full Text - Isolation and propagation of HIV-1 on peripheral blood mononuclear cells | PDF (459 KB) - Isolation and propagation of HIV-1 on peripheral blood mononuclear cells

See also: Protocol by Pannecouque et al.

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae - pp371 - 377

Natalay Kouprina & Vladimir Larionov

Published online: 14 February 2008 | doi:10.1038/nprot.2008.5

Abstract - Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast : Saccharomyces cerevisiae | Full Text - Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae | PDF (224 KB) - Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

A protocol for a lung neovascularization model in rodents - pp378 - 387

Rosemary C Jones, Diane Capen, Bodil Petersen, Rakesh K Jain & Dan G Duda

Published online: 21 February 2008 | doi:10.1038/nprot.2007.536

Abstract - A protocol for a lung neovascularization model in rodents | Full Text - A protocol for a lung neovascularization model in rodents | PDF (609 KB) - A protocol for a lung neovascularization model in rodents

See also: Protocol by Jones et al.

A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents - pp388 - 397

Rosemary C Jones, Diane E Capen, Kenneth S Cohen, Lance L Munn, Rakesh K Jain & Dan G Duda

Published online: 21 February 2008 | doi:10.1038/nprot.2007.537

Abstract - A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents | Full Text - A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents | PDF (483 KB) - A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents

See also: Protocol by Jones et al.

Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography - pp398 - 409

Marjorie Brand, Shravanti Rampalli, Chandra-Prakash Chaturvedi & F Jeffrey Dilworth

Published online: 21 February 2008 | doi:10.1038/nprot.2008.8

Abstract - Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography | Full Text - Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography | PDF (284 KB) - Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography

High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants - pp410 - 418

Elibio L Rech, Giovanni R Vianna & Francisco J L Aragão

Published online: 21 February 2008 | doi:10.1038/nprot.2008.9

Abstract - High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants | Full Text - High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants | PDF (474 KB) - High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants

Spatially and temporally controlled electroporation of early chick embryos - pp419 - 426

Octavian Voiculescu, Costis Papanayotou & Claudio D Stern

Published online: 21 February 2008 | doi:10.1038/nprot.2008.10

Abstract - Spatially and temporally controlled electroporation of early chick embryos | Full Text - Spatially and temporally controlled electroporation of early chick embryos | PDF (495 KB) - Spatially and temporally controlled electroporation of early chick embryos | Supplementary information

Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later - pp427 - 434

Christophe Pannecouque, Dirk Daelemans & Erik De Clercq

Published online: 28 February 2008 | doi:10.1038/nprot.2007.517

Abstract - Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later | Full Text - Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later | PDF (289 KB) - Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later

See also: Protocol by van't Wout et al.

Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions - pp435 - 445

David Parker, Manfred Beckmann, David P Enot, David P Overy, Zaira Caracuel Rios, Martin Gilbert, Nicholas Talbot & John Draper

Published online: 28 February 2008 | doi:10.1038/nprot.2007.499

Abstract - Rice blast infection of : Brachypodium distachyon: as a model system to study dynamic host/pathogen interactions | Full Text - Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions | PDF (444 KB) - Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions

See also: Protocol by Beckmann et al., Protocol by Enot et al., Protocol by Overy et al.

Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data - pp446 - 470

David P Enot, Wanchang Lin, Manfred Beckmann, David Parker, David P Overy & John Draper

Published online: 28 February 2008 | doi:10.1038/nprot.2007.511

Abstract - Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data | Full Text - Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data | PDF (751 KB) - Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data

See also: Protocol by Parker et al., Protocol by Beckmann et al., Protocol by Overy et al.

Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints - pp471 - 485

David P Overy, David P Enot, Kathleen Tailliart, Helen Jenkins, David Parker, Manfred Beckmann & John Draper

Published online: 28 February 2008 | doi:10.1038/nprot.2007.512

Abstract - Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints | Full Text - Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints | PDF (718 KB) - Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints

See also: Protocol by Parker et al., Protocol by Beckmann et al., Protocol by Enot et al.

High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry - pp486 - 504

Manfred Beckmann, David Parker, David P Enot, Emilie Duval & John Draper

Published online: 28 February 2008 | doi:10.1038/nprot.2007.500

Abstract - High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry | Full Text - High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry | PDF (564 KB) - High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry

See also: Protocol by Parker et al., Protocol by Enot et al., Protocol by Overy et al.

Quantitative proteomics using stable isotope labeling with amino acids in cell culture - pp505 - 516

H C Harsha, Henrik Molina & Akhilesh Pandey

Published online: 06 March 2008 | doi:10.1038/nprot.2008.2

Abstract - Quantitative proteomics using stable isotope labeling with amino acids in cell culture | Full Text - Quantitative proteomics using stable isotope labeling with amino acids in cell culture | PDF (404 KB) - Quantitative proteomics using stable isotope labeling with amino acids in cell culture

Ambient molecular imaging by desorption electrospray ionization mass spectrometry - pp517 - 524

Justin M Wiseman, Demian R Ifa, Andre Venter & R Graham Cooks

Published online: 06 March 2008 | doi:10.1038/nprot.2008.11

Abstract - Ambient molecular imaging by desorption electrospray ionization mass spectrometry | Full Text - Ambient molecular imaging by desorption electrospray ionization mass spectrometry | PDF (368 KB) - Ambient molecular imaging by desorption electrospray ionization mass spectrometry

Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography - pp525 - 533

Pietro Speziale, Livia Visai, Simonetta Rindi & Antonella Di Poto

Published online: 06 March 2008 | doi:10.1038/nprot.2008.12

Abstract - Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography | Full Text - Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography | PDF (253 KB) - Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin - pp534 - 545

Mark Howarth & Alice Y Ting

Published online: 06 March 2008 | doi:10.1038/nprot.2008.20

Abstract - Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin | Full Text - Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin | PDF (338 KB) - Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

Preparative scale Baeyer–Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process - pp546 - 554

Iris Hilker, Maria C Gutiérrez, Roland Furstoss, John Ward, Roland Wohlgemuth & Véronique Alphand

Published online: 06 March 2008 | doi:10.1038/nprot.2007.532

Abstract - Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant : Escherichia coli: and : in situ: substrate feeding and product removal process | Full Text - Preparative scale Baeyer–Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process | PDF (311 KB) - Preparative scale Baeyer–Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process