Protocol abstract
Nature Protocols 3, - 341 - 350 (2008)
Published online: 14 February 2008 | doi:10.1038/nprot.2007.529
Subject Categories: Imaging | Isolation, purification and separation | Pharmacology and toxicology | Synthetic chemistry
Preparation of magnetic resonance contrast agents activated by 
-galactosidase
Lauren M Urbanczyk-Pearson1 & Thomas J Meade1
Abstract
The preparation of 
-EgadMe and 
-EgadMe, magnetic resonance (MR) contrast agents that can be used for in vivo detection of -galactosidase, is described. Diastereomerically pure -EgadMe can be synthesized by kinetically resolving the starting material as described in Step 1. The total time for the preparation of the racemic mixture of -EgadMe is about 8 d, and the total time for an diastereomerically resolved agent is about 9 d. The final metallated agent is stable at room temperature as a solid or in aqueous buffer (pH 5.5–10) indefinitely. Diastereomerically pure -EgadMe can be prepared by beginning the synthesis with enantiomerically pure bromopropionic acid. The total time for the preparation of racemic -EgadMe or diastereomerically pure -EgadMe is about 8 d. The final metallated agent is stable at room temperature as a solid or in aqueous buffer (pH 5.5–10) indefinitely.
- Department of Chemistry, Department of Biochemistry and Molecular and Cell Biology, Department of Neurobiology and Physiology, Department of Radiology, Northwestern University, Evanston, Illinois 60201, USA.
Correspondence to: Thomas J Meade1 e-mail: tmeade@northwestern.edu
nature-products
A-Z product listing
- 1 N HCl(Sigma-Aldrich)
- 1,4,7,10-Tetraazacyclododecane(Strem Chemicals)
- 2-Bromopropionic acid(Sigma-Aldrich)
- Acetonitrile(Sigma-Aldrich)
- Ammonium molybdate tetrahydrate(Strem Chemicals)
- Anhydrous acetonitrile(Sigma-Aldrich)