Latest Protocols
Volume 3 No 11
- Archive
In order to view all protocols, including both Nature Protocols and Protocols Network protocols, please browse by category.
To facilitate archiving and citing, Nature Protocols are allocated to publication months and assigned unique page numbers.
About the cover
Nature protocols
Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils - pp1679 - 1692
Christos D Georgiou, Ioannis Papapostolou & Konstantinos Grintzalis
Published online: 09 October 2008 | doi:10.1038/nprot.2008.155
Abstract - | Full Text - Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils | PDF (564 KB) - Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils
Visualization of ternary complexes in living cells by using a BiFC-based FRET assay - pp1693 - 1702
Y John Shyu, Christopher D Suarez & Chang-Deng Hu
Published online: 09 October 2008 | doi:10.1038/nprot.2008.157
Abstract - | Full Text - Visualization of ternary complexes in living cells by using a BiFC-based FRET assay | PDF (606 KB) - Visualization of ternary complexes in living cells by using a BiFC-based FRET assay
Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI - pp1703 - 1708
Yiwen Li, Ying Song, Lian Zhao, Gabriel Gaidosh, Alan M Laties & Rong Wen
Published online: 09 October 2008 | doi:10.1038/nprot.2008.172
Abstract - | Full Text - Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI | PDF (561 KB) - Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI | Supplementary information
Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry - pp1709 - 1717
Namrata D Udeshi, Philip D Compton, Jeffrey Shabanowitz, Donald F Hunt & Kristie L Rose
Published online: 16 October 2008 | doi:10.1038/nprot.2008.159
Abstract - | Full Text - Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry | PDF (384 KB) - Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry
A rapid Percoll gradient procedure for preparation of synaptosomes - pp1718 - 1728
Peter R Dunkley, Paula E Jarvie & Phillip J Robinson
Published online: 16 October 2008 | doi:10.1038/nprot.2008.171
Abstract - | Full Text - A rapid Percoll gradient procedure for preparation of synaptosomes | PDF (4,161 KB) - A rapid Percoll gradient procedure for preparation of synaptosomes
One-way PCR-based mapping of a replication initiation point (RIP) - pp1729 - 1735
Julia Romero & Hoyun Lee
Published online: 16 October 2008 | doi:10.1038/nprot.2008.173
Abstract - | Full Text - One-way PCR-based mapping of a replication initiation point (RIP) | PDF (202 KB) - One-way PCR-based mapping of a replication initiation point (RIP)
DNA cleavage assay for the identification of topoisomerase I inhibitors - pp1736 - 1750
Thomas S Dexheimer & Yves Pommier
Published online: 16 October 2008 | doi:10.1038/nprot.2008.174
Abstract - | Full Text - DNA cleavage assay for the identification of topoisomerase I inhibitors | PDF (627 KB) - DNA cleavage assay for the identification of topoisomerase I inhibitors
Positional cloning by fast-track SNP-mapping in Drosophila melanogaster - pp1751 - 1765
Frank Schnorrer, Annika Ahlford, Doris Chen, Lili Milani & Ann-Christine Syvänen
Published online: 23 October 2008 | doi:10.1038/nprot.2008.175
Abstract - | Full Text - Positional cloning by fast-track SNP-mapping in Drosophila melanogaster | PDF (1,278 KB) - Positional cloning by fast-track SNP-mapping in Drosophila melanogaster | Supplementary information
E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display - pp1766 - 1777
Yariv Mazor, Thomas Van Blarcom, Brent L Iverson & George Georgiou
Published online: 23 October 2008 | doi:10.1038/nprot.2008.176
Abstract - | Full Text - E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display | PDF (412 KB) - E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display
Enzymatic assay for total plasma Cys - pp1778 - 1781
Qinghong Han & Robert M Hoffman
Published online: 23 October 2008 | doi:10.1038/nprot.2008.178
Abstract - | Full Text - Enzymatic assay for total plasma Cys | PDF (201 KB) - Enzymatic assay for total plasma Cys
Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+–ATPase as an example - pp1782 - 1795
Marc le Maire, Bertrand Arnou, Claus Olesen, Dominique Georgin, Christine Ebel & Jesper V Møller
Published online: 30 October 2008 | doi:10.1038/nprot.2008.177
Abstract - | Full Text - Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+–ATPase as an example | PDF (1,737 KB) - Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+–ATPase as an example
Reverse-phase protein lysate microarrays for cell signaling analysis - pp1796 - 1808
Brett Spurrier, Sundhar Ramalingam & Satoshi Nishizuka
Published online: 30 October 2008 | doi:10.1038/nprot.2008.179
Abstract - | Full Text - Reverse-phase protein lysate microarrays for cell signaling analysis | PDF (845 KB) - Reverse-phase protein lysate microarrays for cell signaling analysis
Calibrating excitation light fluxes for quantitative light microscopy in cell biology - pp1809 - 1814
David Grünwald, Shailesh M Shenoy, Sean Burke & Robert H Singer
Published online: 30 October 2008 | doi:10.1038/nprot.2008.180
Abstract - | Full Text - Calibrating excitation light fluxes for quantitative light microscopy in cell biology | PDF (562 KB) - Calibrating excitation light fluxes for quantitative light microscopy in cell biology
