Box 2. Assay of Phospholipid Concentration by the method of Bartlett

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Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+–ATPase as an example

Marc le Maire, Bertrand Arnou, Claus Olesen, Dominique Georgin, Christine Ebel & Jesper V Møller

Nature Protocols 3, 1782 - 1795 (2008) Published online: 30 October 2008

doi:10.1038/nprot.2008.177

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Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+|[ndash]|ATPase as an example