Figure 2 - Single locus segregation patterns in pollen tetrads.

A locus with a transgenic marker construct encoding a fluorescent protein (in this case DsRed2) can be homozygous (A/A) or hemizygous (A/+) for the marker or it can be wild type (+/+). The fluorescence signal in the pollen tetrads will reflect the marker genotype and yield 4:0 (left) 2:2 (middle) or 0:4 (right) pollen tetrads respectively.