Volume 2 Issue 9, September 2007

Protocol

Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

Ben J C Quah
Hilary S Warren
Christopher R Parish
Protocol 23 Aug 2007
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Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

Edwin D Hawkins
Mirja Hommel
Philip D Hodgkin
Protocol 23 Aug 2007
A protocol for designing siRNAs with high functionality and specificity

Amanda Birmingham
Emily Anderson
Anastasia Khvorova
Protocol 23 Aug 2007
Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin γ-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry

Sean S Davies
Venkataraman Amarnath
L Jackson Roberts II
Protocol 23 Aug 2007
Competitive PCR for precise nucleic acid quantification

Lorena Zentilin
Mauro Giacca
Protocol 23 Aug 2007
Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides

Christopher M Strohsahl
Benjamin L Miller
Todd D Krauss
Protocol 23 Aug 2007
Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers

Ina Hubatsch
Eva G E Ragnarsson
Per Artursson
Protocol 23 Aug 2007
Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

Ingrid Remy
F X Campbell-Valois
Stephen W Michnick
Protocol 30 Aug 2007
Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics

Wei L Lee
Junqi Li
Shao Q Yao
Protocol 30 Aug 2007
Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies

Andreas G Tzakos
Laura E Easton
Peter J Lukavsky
Protocol 30 Aug 2007
Application of cationic conjugated polymers in microarrays using label-free DNA targets

ChengJun Sun
Brent S Gaylord
Guillermo C Bazan
Protocol 30 Aug 2007
A computational method of prediction of the end-diastolic pressure–volume relationship by single beat

Stefan Klotz
Marc L Dickstein
Daniel Burkhoff
Protocol 30 Aug 2007
Detergent resistance as a tool in membrane research

Daniel Lingwood
Kai Simons
Protocol 30 Aug 2007
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Vivian I Bonano
Sebastian Oltean
Mariano A Garcia-Blanco
Protocol 30 Aug 2007
Facile synthesis of Ag nanocubes and Au nanocages

Sara E Skrabalak
Leslie Au
Younan Xia
Protocol 06 Sept 2007
Atomic force microscopy and spectroscopy of native membrane proteins

Daniel J Müller
Andreas Engel
Protocol 06 Sept 2007
Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA

Gregory J Cost
Protocol 06 Sept 2007
Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression

Jeannette Achilles
Frank Stahl
Susann Müller
Protocol 13 Sept 2007
The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Frank H Niesen
Helena Berglund
Masoud Vedadi
Protocol 13 Sept 2007
Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation

Bernadett Bacsa
C Oliver Kappe
Protocol 13 Sept 2007
Isolation of DNA from small amounts of elephant ivory

Celia Mailand
Samuel K Wasser
Protocol 06 Sept 2007
Estimation of nuclear DNA content in plants using flow cytometry

Jaroslav Doležel
Johann Greilhuber
Jan Suda
Protocol 06 Sept 2007
Design and implementation of high-throughput RNAi screens in cultured Drosophila cells

Nadire Ramadan
Ian Flockhart
Bernard Mathey-Prevot
Protocol 06 Sept 2007
DNA methylation analysis by pyrosequencing

Jörg Tost
Ivo G Gut
Protocol 06 Sept 2007
Changing medium and passaging cell lines

John R Masters
Glyn N Stacey
Protocol 13 Sept 2007
Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures

Manuela Friedenberger
Marcus Bode
Walter Schubert
Protocol 13 Sept 2007
Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

Partha Mukhopadhyay
Mohanraj Rajesh
Pál Pacher
Protocol 13 Sept 2007
Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

Ingrid Remy
Galia Ghaddar
Stephen W Michnick
Protocol 13 Sept 2007

Volume 2 Issue 9, September 2007

Protocol

Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

A protocol for designing siRNAs with high functionality and specificity

Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin γ-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry

Competitive PCR for precise nucleic acid quantification

Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides

Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers

Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics

Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies

Application of cationic conjugated polymers in microarrays using label-free DNA targets

A computational method of prediction of the end-diastolic pressure–volume relationship by single beat

Detergent resistance as a tool in membrane research

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Facile synthesis of Ag nanocubes and Au nanocages

Atomic force microscopy and spectroscopy of native membrane proteins

Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA

Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation

Isolation of DNA from small amounts of elephant ivory

Estimation of nuclear DNA content in plants using flow cytometry

Design and implementation of high-throughput RNAi screens in cultured Drosophila cells

DNA methylation analysis by pyrosequencing

Changing medium and passaging cell lines

Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

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