Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester - pp2049 - 2056

Ben J C Quah, Hilary S Warren & Christopher R Parish

Published online: 23 August 2007 | doi:10.1038/nprot.2007.296

Abstract - Monitoring lymphocyte proliferation : in vitro: and : in vivo: with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester | Full Text - Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester | PDF (485 KB) - Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

See also: Protocol by Hawkins et al.

Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data - pp2057 - 2067

Edwin D Hawkins, Mirja Hommel, Marian L Turner, Francis L Battye, John F Markham & Philip D Hodgkin

Published online: 23 August 2007 | doi:10.1038/nprot.2007.297

Abstract - Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data | Full Text - Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data | PDF (348 KB) - Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

See also: Protocol by Quah et al.

A protocol for designing siRNAs with high functionality and specificity - pp2068 - 2078

Amanda Birmingham, Emily Anderson, Kevin Sullivan, Angela Reynolds, Queta Boese, Devin Leake, Jon Karpilow & Anastasia Khvorova

Published online: 23 August 2007 | doi:10.1038/nprot.2007.278

Abstract - A protocol for designing siRNAs with high functionality and specificity | Full Text - A protocol for designing siRNAs with high functionality and specificity | PDF (511 KB) - A protocol for designing siRNAs with high functionality and specificity | Supplementary information

Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin -ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry - pp2079 - 2091

Sean S Davies, Venkataraman Amarnath, Cynthia J Brame, Olivier Boutaud & L Jackson Roberts, II

Published online: 23 August 2007 | doi:10.1038/nprot.2007.298

Abstract - Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin [gamma]-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry | Full Text - Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin -ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry | PDF (485 KB) - Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin -ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry

Competitive PCR for precise nucleic acid quantification - pp2092 - 2104

Lorena Zentilin & Mauro Giacca

Published online: 23 August 2007 | doi:10.1038/nprot.2007.299

Abstract - Competitive PCR for precise nucleic acid quantification | Full Text - Competitive PCR for precise nucleic acid quantification | PDF (324 KB) - Competitive PCR for precise nucleic acid quantification

Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides - pp2105 - 2110

Christopher M Strohsahl, Benjamin L Miller & Todd D Krauss

Published online: 23 August 2007 | doi:10.1038/nprot.2007.301

Abstract - Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides | Full Text - Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides | PDF (334 KB) - Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides

Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers - pp2111 - 2119

Ina Hubatsch, Eva G E Ragnarsson & Per Artursson

Published online: 23 August 2007 | doi:10.1038/nprot.2007.303

Abstract - Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers | Full Text - Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers | PDF (304 KB) - Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers

Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase - pp2120 - 2125

Ingrid Remy, F X Campbell-Valois & Stephen W Michnick

Published online: 30 August 2007 | doi:10.1038/nprot.2007.266

Abstract - Detection of protein-protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase | Full Text - Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase | PDF (335 KB) - Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

See also: Protocol by Remy et al.

Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics - pp2126 - 2138

Wei L Lee, Junqi Li, Mahesh Uttamchandani, Hongyan Sun & Shao Q Yao

Published online: 30 August 2007 | doi:10.1038/nprot.2007.305

Abstract - Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics | Full Text - Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics | PDF (435 KB) - Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics

Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies - pp2139 - 2147

Andreas G Tzakos, Laura E Easton & Peter J Lukavsky

Published online: 30 August 2007 | doi:10.1038/nprot.2007.306

Abstract - Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies | Full Text - Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies | PDF (225 KB) - Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies

Application of cationic conjugated polymers in microarrays using label-free DNA targets - pp2148 - 2151

ChengJun Sun, Brent S Gaylord, Janice W Hong, Bin Liu & Guillermo C Bazan

Published online: 30 August 2007 | doi:10.1038/nprot.2007.307

Abstract - Application of cationic conjugated polymers in microarrays using label-free DNA targets | Full Text - Application of cationic conjugated polymers in microarrays using label-free DNA targets | PDF (193 KB) - Application of cationic conjugated polymers in microarrays using label-free DNA targets

A computational method of prediction of the end-diastolic pressure–volume relationship by single beat - pp2152 - 2158

Stefan Klotz, Marc L Dickstein & Daniel Burkhoff

Published online: 30 August 2007 | doi:10.1038/nprot.2007.270

Abstract - A computational method of prediction of the end-diastolic pressure-volume relationship by single beat | Full Text - A computational method of prediction of the end-diastolic pressure–volume relationship by single beat | PDF (449 KB) - A computational method of prediction of the end-diastolic pressure–volume relationship by single beat | Supplementary information

Detergent resistance as a tool in membrane research - pp2159 - 2165

Daniel Lingwood & Kai Simons

Published online: 30 August 2007 | doi:10.1038/nprot.2007.294

Abstract - Detergent resistance as a tool in membrane research | Full Text - Detergent resistance as a tool in membrane research | PDF (198 KB) - Detergent resistance as a tool in membrane research

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice - pp2166 - 2181

Vivian I Bonano, Sebastian Oltean & Mariano A Garcia-Blanco

Published online: 30 August 2007 | doi:10.1038/nprot.2007.292

Abstract - A protocol for imaging alternative splicing regulation : in vivo: using fluorescence reporters in transgenic mice | Full Text - A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice | PDF (493 KB) - A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

Facile synthesis of Ag nanocubes and Au nanocages - pp2182 - 2190

Sara E Skrabalak, Leslie Au, Xingde Li & Younan Xia

Published online: 06 September 2007 | doi:10.1038/nprot.2007.326

Abstract - Facile synthesis of Ag nanocubes and Au nanocages | Full Text - Facile synthesis of Ag nanocubes and Au nanocages | PDF (561 KB) - Facile synthesis of Ag nanocubes and Au nanocages

Atomic force microscopy and spectroscopy of native membrane proteins - pp2191 - 2197

Daniel J Müller & Andreas Engel

Published online: 06 September 2007 | doi:10.1038/nprot.2007.309

Abstract - Atomic force microscopy and spectroscopy of native membrane proteins | Full Text - Atomic force microscopy and spectroscopy of native membrane proteins | PDF (384 KB) - Atomic force microscopy and spectroscopy of native membrane proteins

Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA - pp2198 - 2202

Gregory J Cost

Published online: 06 September 2007 | doi:10.1038/nprot.2007.325

Abstract - Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA | Full Text - Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA | PDF (167 KB) - Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA

Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression - pp2203 - 2211

Jeannette Achilles, Frank Stahl, Hauke Harms & Susann Müller

Published online: 13 September 2007 | doi:10.1038/nprot.2007.322

Abstract - Isolation of intact RNA from cytometrically sorted : Saccharomyces cerevisiae: for the analysis of intrapopulation diversity of gene expression | Full Text - Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression | PDF (336 KB) - Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression

See also: Protocol by Doleel et al. | Protocol by Mailand & Wasser

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability - pp2212 - 2221

Frank H Niesen, Helena Berglund & Masoud Vedadi

Published online: 13 September 2007 | doi:10.1038/nprot.2007.321

Abstract - The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability | Full Text - The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability | PDF (526 KB) - The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation - pp2222 - 2227

Bernadett Bacsa & C Oliver Kappe

Published online: 13 September 2007 | doi:10.1038/nprot.2007.300

Abstract - Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation | Full Text - Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation | PDF (222 KB) - Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation

Isolation of DNA from small amounts of elephant ivory - pp2228 - 2232

Celia Mailand & Samuel K Wasser

Published online: 06 September 2007 | doi:10.1038/nprot.2007.318

Abstract - Isolation of DNA from small amounts of elephant ivory | Full Text - Isolation of DNA from small amounts of elephant ivory | PDF (127 KB) - Isolation of DNA from small amounts of elephant ivory

See also: Protocol by Doleel et al. | Protocol by Achilles et al.

Estimation of nuclear DNA content in plants using flow cytometry - pp2233 - 2244

Jaroslav Doleel, Johann Greilhuber & Jan Suda

Published online: 06 September 2007 | doi:10.1038/nprot.2007.310

Abstract - Estimation of nuclear DNA content in plants using flow cytometry | Full Text - Estimation of nuclear DNA content in plants using flow cytometry | PDF (317 KB) - Estimation of nuclear DNA content in plants using flow cytometry

See also: Protocol by Mailand & Wasser | Protocol by Achilles et al.

Design and implementation of high-throughput RNAi screens in cultured Drosophila cells - pp2245 - 2264

Nadire Ramadan, Ian Flockhart, Matthew Booker, Norbert Perrimon & Bernard Mathey-Prevot

Published online: 06 September 2007 | doi:10.1038/nprot.2007.250

Abstract - Design and implementation of high-throughput RNAi screens in cultured : Drosophila: cells | Full Text - Design and implementation of high-throughput RNAi screens in cultured Drosophila cells | PDF (1,603 KB) - Design and implementation of high-throughput RNAi screens in cultured Drosophila cells

DNA methylation analysis by pyrosequencing - pp2265 - 2275

Jörg Tost & Ivo G Gut

Published online: 06 September 2007 | doi:10.1038/nprot.2007.314

Abstract - DNA methylation analysis by pyrosequencing | Full Text - DNA methylation analysis by pyrosequencing | PDF (371 KB) - DNA methylation analysis by pyrosequencing

Changing medium and passaging cell lines - pp2276 - 2284

John R Masters & Glyn N Stacey

Published online: 13 September 2007 | doi:10.1038/nprot.2007.319

Abstract - Changing medium and passaging cell lines | Full Text - Changing medium and passaging cell lines | PDF (216 KB) - Changing medium and passaging cell lines

Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures - pp2285 - 2294

Manuela Friedenberger, Marcus Bode, Andreas Krusche & Walter Schubert

Published online: 13 September 2007 | doi:10.1038/nprot.2007.320

Abstract - Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures | Full Text - Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures | PDF (515 KB) - Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures | Supplementary information

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy - pp2295 - 2301

Partha Mukhopadhyay, Mohanraj Rajesh, György Haskó, Brian J Hawkins, Muniswamy Madesh & Pál Pacher

Published online: 13 September 2007 | doi:10.1038/nprot.2007.327

Abstract - Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy | Full Text - Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy | PDF (371 KB) - Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

Using the -lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions - pp2302 - 2306

Ingrid Remy, Galia Ghaddar & Stephen W Michnick

Published online: 13 September 2007 | doi:10.1038/nprot.2007.356

Abstract - Using the [beta]-lactamase protein-fragment complementation assay to probe dynamic protein-protein interactions | Full Text - Using the -lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions | PDF (358 KB) - Using the -lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

See also: Protocol by Remy et al.