Protocol abstract


Nature Protocols 2, 1963 - 1972 (2007)
Published online: 9 August 2007 | doi:10.1038/nprot.2007.274

Subject Categories: Cell and tissue culture | Isolation, purification and separation | Model organisms | Cell and developmental biology | Genetic modification

Derivation of human embryonic stem cells from single blastomeres

Irina Klimanskaya1, Young Chung1, Sandy Becker1, Shi-Jiang Lu1 & Robert Lanza1


This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3–4 months.

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  1. Advanced Cell Technology, 381 Plantation Street, Worcester, Massachusetts 01605, USA.

Correspondence to: Robert Lanza1 e-mail: rlanza@advancedcell.com

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