Protocol abstract

Nature Protocols 2, 1963 - 1972 (2007)
Published online: 9 August 2007 | doi:10.1038/nprot.2007.274

Subject Categories: Cell and tissue culture | Isolation, purification and separation | Model organisms | Cell and developmental biology | Genetic modification

Derivation of human embryonic stem cells from single blastomeres

Irina Klimanskaya1, Young Chung1, Sandy Becker1, Shi-Jiang Lu1 & Robert Lanza1

This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3–4 months.

  1. Advanced Cell Technology, 381 Plantation Street, Worcester, Massachusetts 01605, USA.

Correspondence to: Robert Lanza1 e-mail:


These links to content published by NPG are automatically generated.


Medicine Blastomeres and stem cells

Nature News and Views (23 Nov 2006)

Many ways to pluripotency

Nature Biotechnology News and Views (01 Oct 2007)