Protocols for growth and development of Dictyostelium discoideum - pp1307 - 1316

Petra Fey, Anthony S Kowal, Pascale Gaudet, Karen E Pilcher & Rex L Chisholm

Published online: 24 May 2007 | doi:10.1038/nprot.2007.178

Abstract - Protocols for growth and development of : Dictyostelium discoideum | Full Text - Protocols for growth and development of Dictyostelium discoideum | PDF (372 KB) - Protocols for growth and development of Dictyostelium discoideum

See also: Protocol by Gaudet et al. | Protocol by Pilcher et al. | Protocol by Pilcher et al.

Transformation of Dictyostelium discoideum with plasmid DNA - pp1317 - 1324

Pascale Gaudet, Karen E Pilcher, Petra Fey & Rex L Chisholm

Published online: 24 May 2007 | doi:10.1038/nprot.2007.179

Abstract - Transformation of : Dictyostelium discoideum: with plasmid DNA | Full Text - Transformation of Dictyostelium discoideum with plasmid DNA | PDF (187 KB) - Transformation of Dictyostelium discoideum with plasmid DNA

See also: Protocol by Fey et al. | Protocol by Pilcher et al. | Protocol by Pilcher et al.

A reliable general purpose method for extracting genomic DNA from Dictyostelium cells - pp1325 - 1328

Karen E Pilcher, Petra Fey, Pascale Gaudet, Anthony S Kowal & Rex L Chisholm

Published online: 24 May 2007 | doi:10.1038/nprot.2007.180

Abstract - A reliable general purpose method for extracting genomic DNA from : Dictyostelium: cells | Full Text - A reliable general purpose method for extracting genomic DNA from Dictyostelium cells | PDF (139 KB) - A reliable general purpose method for extracting genomic DNA from Dictyostelium cells

See also: Protocol by Fey et al. | Protocol by Gaudet et al. | Protocol by Pilcher et al.

A general purpose method for extracting RNA from Dictyostelium cells - pp1329 - 1332

Karen E Pilcher, Pascale Gaudet, Petra Fey, Anthony S Kowal & Rex L Chisholm

Published online: 24 May 2007 | doi:10.1038/nprot.2007.191

Abstract - A general purpose method for extracting RNA from : Dictyostelium: cells | Full Text - A general purpose method for extracting RNA from Dictyostelium cells | PDF (134 KB) - A general purpose method for extracting RNA from Dictyostelium cells

See also: Protocol by Fey et al. | Protocol by Gaudet et al. | Protocol by Pilcher et al.

Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion - pp1333 - 1349

Kai Hilpert, Dirk FH Winkler & Robert EW Hancock

Published online: 24 May 2007 | doi:10.1038/nprot.2007.160

Abstract - Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion | Full Text - Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion | PDF (565 KB) - Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion

A liquid chromatography–mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites - pp1350 - 1355

Xia Xu, Larry K Keefer, Regina G Ziegler & Timothy D Veenstra

Published online: 24 May 2007 | doi:10.1038/nprot.2007.176

Abstract - A liquid chromatography-mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites | Full Text - A liquid chromatography–mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites | PDF (241 KB) - A liquid chromatography–mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites

Isolation of renal proximal tubular brush-border membranes - pp1356 - 1359

Jürg Biber, Bruno Stieger, Gerti Stange & Heini Murer

Published online: 24 May 2007 | doi:10.1038/nprot.2007.156

Abstract - Isolation of renal proximal tubular brush-border membranes | Full Text - Isolation of renal proximal tubular brush-border membranes | PDF (205 KB) - Isolation of renal proximal tubular brush-border membranes

Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates - pp1360 - 1367

Emilie Voirin, Jean-Paul Behr & Mitsuharu Kotera

Published online: 24 May 2007 | doi:10.1038/nprot.2007.177

Abstract - Versatile synthesis of oligodeoxyribonucleotide-oligospermine conjugates | Full Text - Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates | PDF (263 KB) - Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates

Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries - pp1368 - 1386

Raffi Tonikian, Yingnan Zhang, Charles Boone & Sachdev S Sidhu

Published online: 24 May 2007 | doi:10.1038/nprot.2007.151

Abstract - Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries | Full Text - Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries | PDF (874 KB) - Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries

AFLP technology for DNA fingerprinting - pp1387 - 1398

Marnik Vuylsteke, Johan D Peleman & Michiel JT van Eijk

Published online: 31 May 2007 | doi:10.1038/nprot.2007.175

Abstract - AFLP technology for DNA fingerprinting | Full Text - AFLP technology for DNA fingerprinting | PDF (554 KB) - AFLP technology for DNA fingerprinting

See also: Protocol by Vuylsteke et al.

AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis - pp1399 - 1413

Marnik Vuylsteke, Johan D Peleman & Michiel JT van Eijk

Published online: 31 May 2007 | doi:10.1038/nprot.2007.174

Abstract - AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis | Full Text - AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis | PDF (486 KB) - AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis

See also: Protocol by Vuylsteke et al.

Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—a general method for mapping sites of probe modification in proteomes - pp1414 - 1425

Eranthie Weerapana, Anna E Speers & Benjamin F Cravatt

Published online: 31 May 2007 | doi:10.1038/nprot.2007.194

Abstract - Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)[mdash]a general method for mapping sites of probe modification in proteomes | Full Text - Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—a general method for mapping sites of probe modification in proteomes | PDF (299 KB) - Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—a general method for mapping sites of probe modification in proteomes

Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL - pp1426 - 1437

Armaan Koçer, Martin Walko & Ben L Feringa

Published online: 31 May 2007 | doi:10.1038/nprot.2007.196

Abstract - Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL | Full Text - Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL | PDF (372 KB) - Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL

Detection of proteins using a colorimetric bio-barcode assay - pp1438 - 1444

Jwa-Min Nam, Kyung-Jin Jang & Jay T Groves

Published online: 31 May 2007 | doi:10.1038/nprot.2007.201

Abstract - Detection of proteins using a colorimetric bio-barcode assay | Full Text - Detection of proteins using a colorimetric bio-barcode assay | PDF (237 KB) - Detection of proteins using a colorimetric bio-barcode assay

Extraction, purification and analysis of histones - pp1445 - 1457

David Shechter, Holger L Dormann, C David Allis & Sandra B Hake

Published online: 07 June 2007 | doi:10.1038/nprot.2007.202

Abstract - Extraction, purification and analysis of histones | Full Text - Extraction, purification and analysis of histones | PDF (520 KB) - Extraction, purification and analysis of histones

Single-strand conformation polymorphism analysis using capillary array electrophoresis for large-scale mutation detection - pp1458 - 1466

Lars Allan Larsen, Cathrine Jespersgaard & Paal Skytt Andersen

Published online: 14 June 2007 | doi:10.1038/nprot.2007.200

Abstract - Single-strand conformation polymorphism analysis using capillary array electrophoresis for large-scale mutation detection | Full Text - Single-strand conformation polymorphism analysis using capillary array electrophoresis for large-scale mutation detection | PDF (383 KB) - Single-strand conformation polymorphism analysis using capillary array electrophoresis for large-scale mutation detection

Detection of in vivo protein–DNA interactions using DamID in mammalian cells - pp1467 - 1478

Maartje J Vogel, Daniel Peric-Hupkes & Bas van Steensel

Published online: 07 June 2007 | doi:10.1038/nprot.2007.148

Abstract - Detection of : in vivo: protein-DNA interactions using DamID in mammalian cells | Full Text - Detection of in vivo protein–DNA interactions using DamID in mammalian cells | PDF (267 KB) - Detection of in vivo protein–DNA interactions using DamID in mammalian cells

Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo - pp1479 - 1489

Bing Song, Yu Gu, Jin Pu, Brian Reid, Zhiqiang Zhao & Min Zhao

Published online: 07 June 2007 | doi:10.1038/nprot.2007.205

Abstract - Application of direct current electric fields to cells and tissues : in vitro: and modulation of wound electric field : in vivo | Full Text - Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo | PDF (504 KB) - Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo

Isolation and culture of adult neurons and neurospheres - pp1490 - 1498

Gregory J Brewer & John R Torricelli

Published online: 07 June 2007 | doi:10.1038/nprot.2007.207

Abstract - Isolation and culture of adult neurons and neurospheres | Full Text - Isolation and culture of adult neurons and neurospheres | PDF (273 KB) - Isolation and culture of adult neurons and neurospheres

See also: Protocol by Taylor et al.

In vitro methods to prepare astrocyte and motoneuron cultures for the investigation of potential in vivo interactions - pp1499 - 1507

Anna R Taylor, Mac B Robinson & Carol E Milligan

Published online: 07 June 2007 | doi:10.1038/nprot.2007.208

Abstract - In vitro: methods to prepare astrocyte and motoneuron cultures for the investigation of potential : in vivo: interactions | Full Text - In vitro methods to prepare astrocyte and motoneuron cultures for the investigation of potential in vivo interactions | PDF (386 KB) - In vitro methods to prepare astrocyte and motoneuron cultures for the investigation of potential in vivo interactions

See also: Protocol by Brewer & Torricelli

Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections - pp1508 - 1514

Gregor Obernosterer, Javier Martinez & Mattias Alenius

Published online: 14 June 2007 | doi:10.1038/nprot.2007.153

Abstract - Locked nucleic acid-based : in situ: detection of microRNAs in mouse tissue sections | Full Text - Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections | PDF (471 KB) - Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections

Generation of a fluorescently labeled endogenous protein library in living human cells - pp1515 - 1527

Alex Sigal, Tamar Danon, Ariel Cohen, Ron Milo, Naama Geva-Zatorsky, Gila Lustig, Yuvalal Liron, Uri Alon & Natalie Perzov

Published online: 14 June 2007 | doi:10.1038/nprot.2007.197

Abstract - Generation of a fluorescently labeled endogenous protein library in living human cells | Full Text - Generation of a fluorescently labeled endogenous protein library in living human cells | PDF (489 KB) - Generation of a fluorescently labeled endogenous protein library in living human cells

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins - pp1528 - 1535

Thomas GM Schmidt & Arne Skerra

Published online: 14 June 2007 | doi:10.1038/nprot.2007.209

Abstract - The : Strep: -tag system for one-step purification and high-affinity detection or capturing of proteins | Full Text - The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins | PDF (293 KB) - The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings - pp1536 - 1546

Maximillian H Bailor, Catherine Musselman, Alexandar L Hansen, Kush Gulati, Dinshaw J Patel & Hashim M Al-Hashimi

Published online: 14 June 2007 | doi:10.1038/nprot.2007.221

Abstract - Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings | Full Text - Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings | PDF (489 KB) - Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings

Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide - pp1547 - 1555

Seergazhi G Srivatsan & Yitzhak Tor

Published online: 14 June 2007 | doi:10.1038/nprot.2007.222

Abstract - Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide | Full Text - Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide | PDF (247 KB) - Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide

A modified universal fast walking method for single-tube transposon mapping - pp1556 - 1563

Kyl V Myrick & William M Gelbart

Published online: 14 June 2007 | doi:10.1038/nprot.2007.223

Abstract - A modified universal fast walking method for single-tube transposon mapping | Full Text - A modified universal fast walking method for single-tube transposon mapping | PDF (553 KB) - A modified universal fast walking method for single-tube transposon mapping