Establishment of human tumor xenografts in immunodeficient mice - pp247 - 250

Christopher L Morton & Peter J Houghton

Published online: 22 February 2007 | doi:10.1038/nprot.2007.25

Abstract - Establishment of human tumor xenografts in immunodeficient mice | Full Text - Establishment of human tumor xenografts in immunodeficient mice | PDF (111 KB) - Establishment of human tumor xenografts in immunodeficient mice

Synthesis of 3,3'-bis(sulfonato)-4,4'-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity - pp251 - 258

Darcy C Burns, Fuzhong Zhang & G Andrew Woolley

Published online: 08 March 2007 | doi:10.1038/nprot.2007.21

Abstract - Synthesis of 3,3[prime]-bis(sulfonato)-4,4[prime]-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity | Full Text - Synthesis of 3,3'-bis(sulfonato)-4,4'-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity | PDF (283 KB) - Synthesis of 3,3'-bis(sulfonato)-4,4'-bis(chloroacetamido)azobenzene and cysteine cross-linking for photo-control of protein conformation and activity

Isolation of intact vacuoles from Arabidopsis rosette leaf–derived protoplasts - pp259 - 262

Stéphanie Robert, Jan Zouhar, Clay Carter & Natasha Raikhel

Published online: 22 February 2007 | doi:10.1038/nprot.2007.26

Abstract - Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts | Full Text - Isolation of intact vacuoles from Arabidopsis rosette leaf–derived protoplasts | PDF (152 KB) - Isolation of intact vacuoles from Arabidopsis rosette leaf–derived protoplasts

In vivo imaging of single axons in the mouse spinal cord - pp263 - 268

Thomas Misgeld, Ivana Nikic & Martin Kerschensteiner

Published online: 22 February 2007 | doi:10.1038/nprot.2007.24

Abstract - In vivo: imaging of single axons in the mouse spinal cord | Full Text - In vivo imaging of single axons in the mouse spinal cord | PDF (328 KB) - In vivo imaging of single axons in the mouse spinal cord

Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I - pp269 - 276

Anand Patel, Rachel T Noble, Joshua A Steele, Michael S Schwalbach, Ian Hewson & Jed A Fuhrman

Published online: 01 March 2007 | doi:10.1038/nprot.2007.6

Abstract - Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I | Full Text - Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I | PDF (294 KB) - Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I

The parallel rod floor test: a measure of ataxia in mice - pp277 - 281

Helen M Kamens & John C Crabbe

Published online: 22 February 2007 | doi:10.1038/nprot.2007.19

Abstract - The parallel rod floor test: a measure of ataxia in mice | Full Text - The parallel rod floor test: a measure of ataxia in mice | PDF (164 KB) - The parallel rod floor test: a measure of ataxia in mice

See also: Protocol by Walf & Frye

Synthesis of N-succinimidyl 4-guanidinomethyl-3-[^*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides - pp282 - 286

Ganesan Vaidyanathan & Michael R Zalutsky

Published online: 01 March 2007 | doi:10.1038/nprot.2007.20

Abstract - Synthesis of : N: -succinimidyl 4-guanidinomethyl-3-[: [ast]: I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides | Full Text - Synthesis of N-succinimidyl 4-guanidinomethyl-3-[*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides | PDF (179 KB) - Synthesis of N-succinimidyl 4-guanidinomethyl-3-[*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides

Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts - pp287 - 295

Christian Frezza, Sara Cipolat & Luca Scorrano

Published online: 22 February 2007 | doi:10.1038/nprot.2006.478

Abstract - Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts | Full Text - Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts | PDF (301 KB) - Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts

Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) - pp296 - 304

Naoko Yoshida & Anthony CF Perry

Published online: 01 March 2007 | doi:10.1038/nprot.2007.7

Abstract - Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) | Full Text - Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) | PDF (467 KB) - Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) | Supplementary information

Synthesis and site-specific incorporation of a simple fluorescent pyrimidine - pp305 - 316

Nicholas J Greco & Yitzhak Tor

Published online: 01 March 2007 | doi:10.1038/nprot.2006.464

Abstract - Synthesis and site-specific incorporation of a simple fluorescent pyrimidine | Full Text - Synthesis and site-specific incorporation of a simple fluorescent pyrimidine | PDF (325 KB) - Synthesis and site-specific incorporation of a simple fluorescent pyrimidine

Rapid preparation of the mitotic kinesin Eg5 inhibitor monastrol using controlled microwave-assisted synthesis - pp317 - 321

Doris Dallinger & C Oliver Kappe

Published online: 01 March 2007 | doi:10.1038/nprot.2006.436

Abstract - Rapid preparation of the mitotic kinesin Eg5 inhibitor monastrol using controlled microwave-assisted synthesis | Full Text - Rapid preparation of the mitotic kinesin Eg5 inhibitor monastrol using controlled microwave-assisted synthesis | PDF (182 KB) - Rapid preparation of the mitotic kinesin Eg5 inhibitor monastrol using controlled microwave-assisted synthesis

The use of the elevated plus maze as an assay of anxiety-related behavior in rodents - pp322 - 328

Alicia A Walf & Cheryl A Frye

Published online: 01 March 2007 | doi:10.1038/nprot.2007.44

Abstract - The use of the elevated plus maze as an assay of anxiety-related behavior in rodents | Full Text - The use of the elevated plus maze as an assay of anxiety-related behavior in rodents | PDF (209 KB) - The use of the elevated plus maze as an assay of anxiety-related behavior in rodents

See also: Protocol by Kamens & Crabbe

In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro - pp329 - 333

Chun-Chi Liang, Ann Y Park & Jun-Lin Guan

Published online: 01 March 2007 | doi:10.1038/nprot.2007.30

Abstract - In vitro: scratch assay: a convenient and inexpensive method for analysis of cell migration : in vitro | Full Text - In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro | PDF (213 KB) - In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro

Solid-phase extraction of N-linked glycopeptides - pp334 - 339

Yuan Tian, Yong Zhou, Sarah Elliott, Ruedi Aebersold & Hui Zhang

Published online: 01 March 2007 | doi:10.1038/nprot.2007.42

Abstract - Solid-phase extraction of : N: -linked glycopeptides | Full Text - Solid-phase extraction of N-linked glycopeptides | PDF (197 KB) - Solid-phase extraction of N-linked glycopeptides

Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products - pp340 - 346

Sébastien F Emonet, Gilda Grard, Nadège M Brisbarre, Grégory N Moureau, Sarah Temmam, Rémi N Charrel & Xavier de Lamballerie

Published online: 01 March 2007 | doi:10.1038/nprot.2006.453

Abstract - Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products | Full Text - Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products | PDF (266 KB) - Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products

Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines - pp347 - 356

Alejandro Soto-Gutiérrez, Nalu Navarro-Álvarez, Debiao Zhao, Jorge D Rivas-Carrillo, Jane Lebkowski, Noriaki Tanaka, Ira J Fox & Naoya Kobayashi

Published online: 08 March 2007 | doi:10.1038/nprot.2007.18

Abstract - Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines | Full Text - Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines | PDF (522 KB) - Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines | Supplementary information

DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications - pp357 - 371

Brian M Zeglis & Jacqueline K Barton

Published online: 08 March 2007 | doi:10.1038/nprot.2007.22

Abstract - DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications | Full Text - DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications | PDF (641 KB) - DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications

Cytogenetic harvesting of commonly used tumor cell lines - pp372 - 382

Roderick A F MacLeod, Maren Kaufmann & Hans G Drexler

Published online: 08 March 2007 | doi:10.1038/nprot.2007.29

Abstract - Cytogenetic harvesting of commonly used tumor cell lines | Full Text - Cytogenetic harvesting of commonly used tumor cell lines | PDF (517 KB) - Cytogenetic harvesting of commonly used tumor cell lines | Supplementary information

A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His₆-maltose binding protein fusion tag - pp383 - 391

Sreedevi Nallamsetty & David S Waugh

Published online: 08 March 2007 | doi:10.1038/nprot.2007.50

Abstract - A generic protocol for the expression and purification of recombinant proteins in : Escherichia coli: using a combinatorial His: 6: -maltose binding protein fusion tag | Full Text - A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag | PDF (249 KB) - A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

Reverse transfection on cell arrays for high content screening microscopy - pp392 - 399

Holger Erfle, Beate Neumann, Urban Liebel, Phill Rogers, Michael Held, Thomas Walter, Jan Ellenberg & Rainer Pepperkok

Published online: 01 March 2007 | doi:10.1038/nprot.2006.483

Abstract - Reverse transfection on cell arrays for high content screening microscopy | Full Text - Reverse transfection on cell arrays for high content screening microscopy | PDF (584 KB) - Reverse transfection on cell arrays for high content screening microscopy

Analysis and interpretation of quadratic models of receptive fields - pp400 - 407

Pietro Berkes & Laurenz Wiskott

Published online: 08 March 2007 | doi:10.1038/nprot.2007.27

Abstract - Analysis and interpretation of quadratic models of receptive fields | Full Text - Analysis and interpretation of quadratic models of receptive fields | PDF (334 KB) - Analysis and interpretation of quadratic models of receptive fields

Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells - pp408 - 415

Mi Hee Lim

Published online: 08 March 2007 | doi:10.1038/nprot.2007.43

Abstract - Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells | Full Text - Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells | PDF (215 KB) - Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells

Molecular PET imaging of HSV1-tk reporter gene expression using [¹⁸F]FEAU - pp416 - 423

Suren Soghomonyan, Amin Hajitou, Roberto Rangel, Martin Trepel, Renata Pasqualini, Wadih Arap, Juri G Gelovani & Mian M Alauddin

Published online: 08 March 2007 | doi:10.1038/nprot.2007.49

Abstract - Molecular PET imaging of : HSV1-tk: reporter gene expression using [: 18: F]FEAU | Full Text - Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU | PDF (267 KB) - Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU

Substrate activity screening (SAS): a general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery - pp424 - 433

Andrew W Patterson, Warren J L Wood & Jonathan A Ellman

Published online: 08 March 2007 | doi:10.1038/nprot.2007.28

Abstract - Substrate activity screening (SAS): a general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery | Full Text - Substrate activity screening (SAS): a general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery | PDF (233 KB) - Substrate activity screening (SAS): a general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery

Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches - pp434 - 448

Espen Hartveit & Margaret Lin Veruki

Published online: 08 March 2007 | doi:10.1038/nprot.2007.47

Abstract - Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches | Full Text - Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches | PDF (541 KB) - Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches | Supplementary information

Preparation of integrin (v)(3)-targeting Ab 38C2 constructs - pp449 - 456

Subhash C Sinha, Sanjib Das, Lian-Sheng Li, Richard A Lerner & Carlos F Barbas, III

Published online: 08 March 2007 | doi:10.1038/nprot.2007.3

Abstract - Preparation of integrin [alpha](v)[beta](3)-targeting Ab 38C2 constructs | Full Text - Preparation of integrin (v)(3)-targeting Ab 38C2 constructs | PDF (364 KB) - Preparation of integrin (v)(3)-targeting Ab 38C2 constructs