Table 1
From the following article
Isolation of intact vacuoles from Arabidopsis rosette leaf–derived protoplasts
Stéphanie Robert, Jan Zouhar, Clay Carter & Natasha Raikhel
Nature Protocols 2, 259 - 262 (2007) Published online: 22 February 2007
doi:10.1038/nprot.2007.26
Table 1. Troubleshooting table.
| Step | Problem | Possible reasons | Solution |
|---|---|---|---|
| 1 | No vacuoles detected at the interface | The amount of tissue was exceeded | Use 2 g of leaves |
| 22 | No vacuoles detected at the interface | The Ficoll stock solution was overheated | Avoid excessive heat |
| 23 | A few vacuoles detected at the interface | The plant material has aged and is senescing | Use fresh, 35-day-old rosette leaves |
