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Protocol
Nature Protocols 2, 3153–3165 (1 December 2007) | doi:10.1038/nprot.2007.471
Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection
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Abstract
Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system combines the advantages of viruses and naked DNA molecules for gene therapy purposes; however, efficacious delivery of DNA molecules to animal tissues can still be problematic. Here we describe the hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse. The procedure involves rapid, high-pressure injection of a DNA solution into the tail vein. The overall procedure takes
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