Latest Protocols
Volume 2 No 12
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Nature protocols
Imaging activation of adult-generated granule cells in spatial memory - pp3033 - 3044
Nohjin Kee, Cátia M Teixeira, Afra H Wang & Paul W Frankland
Published online: 21 November 2007 | doi:10.1038/nprot.2007.415
Abstract - | Full Text - Imaging activation of adult-generated granule cells in spatial memory | PDF (345 KB) - Imaging activation of adult-generated granule cells in spatial memory
Measurement of cell proliferation by heavy water labeling - pp3045 - 3057
Robert Busch, Richard A Neese, Mohamad Awada, Gregory M Hayes & Marc K Hellerstein
Published online: 21 November 2007 | doi:10.1038/nprot.2007.420
Abstract - | Full Text - Measurement of cell proliferation by heavy water labeling | PDF (325 KB) - Measurement of cell proliferation by heavy water labeling
Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio–mass spectrometric analysis - pp3058 - 3062
Jason N Voogt, Mohamad Awada, Elizabeth J Murphy, Gregory M Hayes, Robert Busch & Marc K Hellerstein
Published online: 21 November 2007 | doi:10.1038/nprot.2007.421
Abstract - | Full Text - Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio–mass spectrometric analysis | PDF (166 KB) - Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio–mass spectrometric analysis
Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules - pp3063 - 3073
Steffen U Eisenhardt, Meike Schwarz, Nicole Bassler & Karlheinz Peter
Published online: 21 November 2007 | doi:10.1038/nprot.2007.455
Abstract - | Full Text - Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules | PDF (355 KB) - Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules
Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila - pp3074 - 3080
Nikolay Ninov & Enrique Martín-Blanco
Published online: 29 November 2007 | doi:10.1038/nprot.2007.417
Abstract - | Full Text - Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila | PDF (423 KB) - Live imaging of epidermal morphogenesis during the development of the adult abdominal epidermis of Drosophila | Supplementary information
Induction of pluripotent stem cells from fibroblast cultures - pp3081 - 3089
Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa & Shinya Yamanaka
Published online: 29 November 2007 | doi:10.1038/nprot.2007.418
Abstract - | Full Text - Induction of pluripotent stem cells from fibroblast cultures | PDF (433 KB) - Induction of pluripotent stem cells from fibroblast cultures
Efficient transfection of DNA or shRNA vectors into neurons using magnetofection - pp3090 - 3101
Thomas Buerli, Christophe Pellegrino, Kristin Baer, Barbara Lardi-Studler, Ilona Chudotvorova, Jean-Marc Fritschy, Igor Medina & Christian Fuhrer
Published online: 29 November 2007 | doi:10.1038/nprot.2007.445
Abstract - | Full Text - Efficient transfection of DNA or shRNA vectors into neurons using magnetofection | PDF (852 KB) - Efficient transfection of DNA or shRNA vectors into neurons using magnetofection
The Bead blot: a method for selecting small molecule ligands for protein capture and purification - pp3102 - 3110
Julia Tait Lathrop & David Hammond
Published online: 29 November 2007 | doi:10.1038/nprot.2007.450
Abstract - | Full Text - The Bead blot: a method for selecting small molecule ligands for protein capture and purification | PDF (309 KB) - The Bead blot: a method for selecting small molecule ligands for protein capture and purification
Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange - pp3111 - 3114
Cristiano Ferlini & Giovanni Scambia
Published online: 06 December 2007 | doi:10.1038/nprot.2007.397
Abstract - | Full Text - Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange | PDF (187 KB) - Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange
Pd-catalyzed Suzuki–Miyaura reactions of aryl halides using bulky biarylmonophosphine ligands - pp3115 - 3121
Ryan A Altman & Stephen L Buchwald
Published online: 06 December 2007 | doi:10.1038/nprot.2007.411
Abstract - | Full Text - Pd-catalyzed Suzuki–Miyaura reactions of aryl halides using bulky biarylmonophosphine ligands | PDF (337 KB) - Pd-catalyzed Suzuki–Miyaura reactions of aryl halides using bulky biarylmonophosphine ligands
Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage - pp3122 - 3135
James W Gaynor, Joanne Bentley & Richard Cosstick
Published online: 06 December 2007 | doi:10.1038/nprot.2007.451
Abstract - | Full Text - Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage | PDF (292 KB) - Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage
Profiling of microRNA expression by mRAP - pp3136 - 3145
Shuji Takada & Hiroyuki Mano
Published online: 06 December 2007 | doi:10.1038/nprot.2007.457
Abstract - | Full Text - Profiling of microRNA expression by mRAP | PDF (364 KB) - Profiling of microRNA expression by mRAP
Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system - pp3146 - 3152
Lalitha R Belur, Kelly Podetz-Pedersen, Joel Frandsen & R Scott McIvor
Published online: 06 December 2007 | doi:10.1038/nprot.2007.460
Abstract - | Full Text - Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system | PDF (243 KB) - Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system
Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection - pp3153 - 3165
Jason B Bell, Kelly M Podetz-Pedersen, Elena L Aronovich, Lalitha R Belur, R Scott McIvor & Perry B Hackett
Published online: 06 December 2007 | doi:10.1038/nprot.2007.471
Abstract - | Full Text - Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection | PDF (761 KB) - Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection
Circular dichroism to determine binding mode and affinity of ligand–DNA interactions - pp3166 - 3172
Nichola C Garbett, Patricia A Ragazzon & Jonathan B Chaires
Published online: 06 December 2007 | doi:10.1038/nprot.2007.475
Abstract - | Full Text - Circular dichroism to determine binding mode and affinity of ligand–DNA interactions | PDF (274 KB) - Circular dichroism to determine binding mode and affinity of ligand–DNA interactions
Single-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis - pp3173 - 3184
Jochen B Geigl & Michael R Speicher
Published online: 06 December 2007 | doi:10.1038/nprot.2007.476
Abstract - | Full Text - Single-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis | PDF (344 KB) - Single-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis
Site-selective glycosylation of proteins: creating synthetic glycoproteins - pp3185 - 3194
Sander I van Kasteren, Holger B Kramer, David P Gamblin & Benjamin G Davis
Published online: 06 December 2007 | doi:10.1038/nprot.2007.430
Abstract - | Full Text - Site-selective glycosylation of proteins: creating synthetic glycoproteins | PDF (417 KB) - Site-selective glycosylation of proteins: creating synthetic glycoproteins
Synthesis of cationic single-isomer cyclodextrins for the chiral separation of amino acids and anionic pharmaceuticals - pp3195 - 3200
Weihua Tang & Siu-Choon Ng
Published online: 06 December 2007 | doi:10.1038/nprot.2007.479
Abstract - | Full Text - Synthesis of cationic single-isomer cyclodextrins for the chiral separation of amino acids and anionic pharmaceuticals | PDF (169 KB) - Synthesis of cationic single-isomer cyclodextrins for the chiral separation of amino acids and anionic pharmaceuticals
Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization - pp3201 - 3209
Matthieu Sainlos & Barbara Imperiali
Published online: 13 December 2007 | doi:10.1038/nprot.2007.442
Abstract - | Full Text - Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization | PDF (395 KB) - Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization
Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach - pp3210 - 3218
Matthieu Sainlos & Barbara Imperiali
Published online: 13 December 2007 | doi:10.1038/nprot.2007.443
Abstract - | Full Text - Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach | PDF (252 KB) - Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach
Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN - pp3219 - 3225
Matthieu Sainlos & Barbara Imperiali
Published online: 13 December 2007 | doi:10.1038/nprot.2007.444
Abstract - | Full Text - Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN | PDF (168 KB) - Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN
Construction and calibration of an optical trap on a fluorescence optical microscope - pp3226 - 3238
Woei Ming Lee, Peter J Reece, Robert F Marchington, Nikolaus K Metzger & Kishan Dholakia
Published online: 13 December 2007 | doi:10.1038/nprot.2007.446
Abstract - | Full Text - Construction and calibration of an optical trap on a fluorescence optical microscope | PDF (558 KB) - Construction and calibration of an optical trap on a fluorescence optical microscope | Supplementary information
Preparation of macromolecular complexes for cryo-electron microscopy - pp3239 - 3246
Robert A Grassucci, Derek J Taylor & Joachim Frank
Published online: 13 December 2007 | doi:10.1038/nprot.2007.452
Abstract - | Full Text - Preparation of macromolecular complexes for cryo-electron microscopy | PDF (565 KB) - Preparation of macromolecular complexes for cryo-electron microscopy
Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences - pp3247 - 3256
Irene Coin, Michael Beyermann & Michael Bienert
Published online: 13 December 2007 | doi:10.1038/nprot.2007.454
Abstract - | Full Text - Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences | PDF (286 KB) - Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences
Silencing of mammalian genes by tetracycline-inducible shRNA expression - pp3257 - 3269
Sven Kappel, Yves Matthess, Manfred Kaufmann & Klaus Strebhardt
Published online: 13 December 2007 | doi:10.1038/nprot.2007.458
Abstract - | Full Text - Silencing of mammalian genes by tetracycline-inducible shRNA expression | PDF (382 KB) - Silencing of mammalian genes by tetracycline-inducible shRNA expression
Purification and characterization of transcribed RNAs using gel filtration chromatography - pp3270 - 3277
Sean A McKenna, Insil Kim, Elisabetta Viani Puglisi, Darrin A Lindhout, Colin Echeverría Aitken, R Andrew Marshall & Joseph D Puglisi
Published online: 13 December 2007 | doi:10.1038/nprot.2007.480
Abstract - | Full Text - Purification and characterization of transcribed RNAs using gel filtration chromatography | PDF (244 KB) - Purification and characterization of transcribed RNAs using gel filtration chromatography
Detecting protein–protein interactions by far western blotting - pp3278 - 3284
Yuliang Wu, Qiang Li & Xing-Zhen Chen
Published online: 13 December 2007 | doi:10.1038/nprot.2007.459
Abstract - | Full Text - Detecting protein–protein interactions by far western blotting | PDF (189 KB) - Detecting protein–protein interactions by far western blotting
Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses - pp3285 - 3298
Petra Füger, Laila B Behrends, Sara Mertel, Stephan J Sigrist & Tobias M Rasse
Published online: 13 December 2007 | doi:10.1038/nprot.2007.472
Abstract - | Full Text - Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses | PDF (552 KB) - Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses | Supplementary information