Table 1
From the following article
Detecting protein–protein interactions by far western blotting
Yuliang Wu, Qiang Li & Xing-Zhen Chen
Nature Protocols 2, 3278 - 3284 (2007) Published online: 13 December 2007
doi:10.1038/nprot.2007.459
Table 1. AC buffer with varying concentrations of guanidine–HCl used in denaturing and renaturing.
| Concentration of guanidine–HCl (M) | 6 | 3 | 1 | 0.1 | 0 |
|---|---|---|---|---|---|
| Glycerol (ml) | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| 5 M NaCl (ml) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| 1 M Tris, pH 7.5 (ml) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| 0.5 M EDTA (ml) | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| 10% Tween-20 (ml) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Guanidine–HCl (8 M) (ml) | 18.75 | 9.30 | 3.13 | 0.31 | 0 |
| Milk powder (g) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
1 M DTT ( l) | 25 | 25 | 25 | 25 | 25 |
| ddH2O (ml) | 2.45 | 12.82 | 18.07 | 20.89 | 21.20 |
| Total volume (ml) | 25 | 25 | 25 | 25 | 25 |
| Time/temperature | 30 min/room temperature (RT) | 30 min/RT | 30 min/RT | 30 min/4 °C | 1 h overnight/4 °C |