Figure 2 - Expression and purification of polypeptides.
From the following article
Detecting protein–protein interactions by far western blotting
Yuliang Wu, Qiang Li & Xing-Zhen Chen
Nature Protocols 2, 3278 - 3284 (2007) Published online: 13 December 2007
doi:10.1038/nprot.2007.459
The soluble C termini of KIF3A (KIF3AC, aa 403–702), of KIF3B (KIF3BC, aa 407–747), of polycystin-2 (PC2C, aa 682–968) and of fibrocystin (FPCC, aa 3882–4074) were used in this study. (a) His-tagged KIF3BC and KIF3AC proteins were purified from bacteria and stained by Coomassie brilliant blue (CBB). (b) Escherichia coli extracts expressing glutathione S-transferase (GST) alone, GST-PC2C or GST-FPCC, were subjected to western blotting (WB) with an antibody against GST. (c) GFP and GFP–FPCC were stably expressed in MDCK cells. Shown are WB data using an anti-GFP antibody. BSA acts as a negative control.