Latest Protocols
Volume 2 No 11
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Nature protocols
Silver staining DNA in polyacrylamide gels - pp2649 - 2654
Brant J Bassam & Peter M Gresshoff
Published online: 25 October 2007 | doi:10.1038/nprot.2007.330
Abstract - Silver staining DNA in polyacrylamide gels | Full Text - Silver staining DNA in polyacrylamide gels | PDF (221 KB) - Silver staining DNA in polyacrylamide gels
Methods of using click chemistry in the discovery of enzyme inhibitors - pp2655 - 2664
Rajavel Srinivasan, Junqi Li, Su Ling Ng, Karunakaran A Kalesh & Shao Q Yao
Published online: 25 October 2007 | doi:10.1038/nprot.2007.323
Abstract - Methods of using click chemistry in the discovery of enzyme inhibitors | Full Text - Methods of using click chemistry in the discovery of enzyme inhibitors | PDF (474 KB) - Methods of using click chemistry in the discovery of enzyme inhibitors
An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis–Hillman bromides in aqueous media - pp2665 - 2676
Deevi Basavaiah, Kanumuri Ramesh Reddy & Nagaswamy Kumaragurubaran
Published online: 25 October 2007 | doi:10.1038/nprot.2007.369
Abstract - An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis-Hillman bromides in aqueous media | Full Text - An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis–Hillman bromides in aqueous media | PDF (284 KB) - An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis–Hillman bromides in aqueous media | Supplementary information
Probe selection for DNA microarrays using OligoWiz - pp2677 - 2691
Rasmus Wernersson, Agnieszka S Juncker & Henrik Bjørn Nielsen
Published online: 25 October 2007 | doi:10.1038/nprot.2007.370
Abstract - Probe selection for DNA microarrays using OligoWiz | Full Text - Probe selection for DNA microarrays using OligoWiz | PDF (1,017 KB) - Probe selection for DNA microarrays using OligoWiz
Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts - pp2692 - 2703
Olaf Beckonert, Hector C Keun, Timothy M D Ebbels, Jacob Bundy, Elaine Holmes, John C Lindon & Jeremy K Nicholson
Published online: 25 October 2007 | doi:10.1038/nprot.2007.376
Abstract - Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts | Full Text - Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts | PDF (418 KB) - Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts | Supplementary information
Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase - pp2704 - 2711
Yao-Wen Wu, Kirill Alexandrov & Luc Brunsveld
Published online: 25 October 2007 | doi:10.1038/nprot.2007.401
Abstract - Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase | Full Text - Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase | PDF (227 KB) - Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase | Supplementary information
A method for quantifying aggression in male Drosophila melanogaster - pp2712 - 2718
Herman A Dierick
Published online: 25 October 2007 | doi:10.1038/nprot.2007.404
Abstract - A method for quantifying aggression in male : Drosophila melanogaster | Full Text - A method for quantifying aggression in male Drosophila melanogaster | PDF (269 KB) - A method for quantifying aggression in male Drosophila melanogaster | Supplementary information
Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry - pp2719 - 2727
Leonarda Troiano, Roberta Ferraresi, Enrico Lugli, Elisa Nemes, Erika Roat, Milena Nasi, Marcello Pinti & Andrea Cossarizza
Published online: 25 October 2007 | doi:10.1038/nprot.2007.405
Abstract - Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry | Full Text - Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry | PDF (444 KB) - Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry
Version 1.2 of the Crystallography and NMR system - pp2728 - 2733
Axel T Brunger
Published online: 25 October 2007 | doi:10.1038/nprot.2007.406
Abstract - Version 1.2 of the Crystallography and NMR system | Full Text - Version 1.2 of the Crystallography and NMR system | PDF (631 KB) - Version 1.2 of the Crystallography and NMR system | Supplementary information
Genetic knockouts and knockins in human somatic cells - pp2734 - 2746
Carlo Rago, Bert Vogelstein & Fred Bunz
Published online: 25 October 2007 | doi:10.1038/nprot.2007.408
Abstract - Genetic knockouts and knockins in human somatic cells | Full Text - Genetic knockouts and knockins in human somatic cells | PDF (331 KB) - Genetic knockouts and knockins in human somatic cells
Fabrication of carbohydrate chips and their use to probe protein–carbohydrate interactions - pp2747 - 2758
Sungjin Park, Myung-Ryul Lee & Injae Shin
Published online: 01 November 2007 | doi:10.1038/nprot.2007.373
Abstract - Fabrication of carbohydrate chips and their use to probe protein-carbohydrate interactions | Full Text - Fabrication of carbohydrate chips and their use to probe protein–carbohydrate interactions | PDF (327 KB) - Fabrication of carbohydrate chips and their use to probe protein–carbohydrate interactions | Supplementary information
Preparation of chiral derivatives of 
-Ala containing the 
-phenylethyl group: useful starting materials for the asymmetric synthesis of -amino acids - pp2759 - 2766
Ramón Guzmán-Mejía, Gloria Reyes-Rangel & Eusebio Juaristi
Published online: 01 November 2007 | doi:10.1038/nprot.2007.375
Abstract - Preparation of chiral derivatives of [beta]-Ala containing the [alpha]-phenylethyl group: useful starting materials for the asymmetric synthesis of [beta]-amino acids | Full Text - Preparation of chiral derivatives of 
-Ala containing the 
-phenylethyl group: useful starting materials for the asymmetric synthesis of -amino acids | PDF (369 KB) - Preparation of chiral derivatives of -Ala containing the -phenylethyl group: useful starting materials for the asymmetric synthesis of -amino acids
Synthesis of poly(L-lactide) and polyglycolide by ring-opening polymerization - pp2767 - 2771
Sachiko Kaihara, Shuichi Matsumura, Antonios G Mikos & John P Fisher
Published online: 01 November 2007 | doi:10.1038/nprot.2007.391
Abstract - Synthesis of poly(: L: -lactide) and polyglycolide by ring-opening polymerization | Full Text - Synthesis of poly(L-lactide) and polyglycolide by ring-opening polymerization | PDF (225 KB) - Synthesis of poly(L-lactide) and polyglycolide by ring-opening polymerization
The 32P-postlabeling assay for DNA adducts - pp2772 - 2781
David H Phillips & Volker M Arlt
Published online: 01 November 2007 | doi:10.1038/nprot.2007.394
Abstract - The : 32: P-postlabeling assay for DNA adducts | Full Text - The 32P-postlabeling assay for DNA adducts | PDF (428 KB) - The 32P-postlabeling assay for DNA adducts
Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences - pp2782 - 2795
Omar Bagasra
Published online: 01 November 2007 | doi:10.1038/nprot.2007.395
Abstract - Protocols for the : in situ: PCR-amplification and detection of mRNA and DNA sequences | Full Text - Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences | PDF (740 KB) - Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences
Predicting the age of mosquitoes using transcriptional profiles - pp2796 - 2806
Peter E Cook, Leon E Hugo, Iñaki Iturbe-Ormaetxe, Craig R Williams, Stephen F Chenoweth, Scott A Ritchie, Peter A Ryan, Brian H Kay, Mark W Blows & Scott L O'Neill
Published online: 01 November 2007 | doi:10.1038/nprot.2007.396
Abstract - Predicting the age of mosquitoes using transcriptional profiles | Full Text - Predicting the age of mosquitoes using transcriptional profiles | PDF (626 KB) - Predicting the age of mosquitoes using transcriptional profiles | Supplementary information
A protocol for the in vitro selection of specific oligonucleotide probes for high-resolution DNA typing - pp2807 - 2814
Ivan Brukner, Maja Krajinovic, André Dascal & Damian Labuda
Published online: 01 November 2007 | doi:10.1038/nprot.2007.398
Abstract - A protocol for the : in vitro: selection of specific oligonucleotide probes for high-resolution DNA typing | Full Text - A protocol for the in vitro selection of specific oligonucleotide probes for high-resolution DNA typing | PDF (341 KB) - A protocol for the in vitro selection of specific oligonucleotide probes for high-resolution DNA typing
Retrovirally mediated telomerase immortalization of neural progenitor cells - pp2815 - 2825
Neeta S Roy, Devin Chandler-Militello, Gang Lu, Su Wang & Steven A Goldman
Published online: 01 November 2007 | doi:10.1038/nprot.2007.402
Abstract - Retrovirally mediated telomerase immortalization of neural progenitor cells | Full Text - Retrovirally mediated telomerase immortalization of neural progenitor cells | PDF (386 KB) - Retrovirally mediated telomerase immortalization of neural progenitor cells
Single-channel recording of ligand-gated ion channels - pp2826 - 2841
Martin Mortensen & Trevor G Smart
Published online: 01 November 2007 | doi:10.1038/nprot.2007.403
Abstract - Single-channel recording of ligand-gated ion channels | Full Text - Single-channel recording of ligand-gated ion channels | PDF (493 KB) - Single-channel recording of ligand-gated ion channels
Practical guidelines for dual-color fluorescence cross-correlation spectroscopy - pp2842 - 2856
Kirsten Bacia & Petra Schwille
Published online: 01 November 2007 | doi:10.1038/nprot.2007.410
Abstract - Practical guidelines for dual-color fluorescence cross-correlation spectroscopy | Full Text - Practical guidelines for dual-color fluorescence cross-correlation spectroscopy | PDF (467 KB) - Practical guidelines for dual-color fluorescence cross-correlation spectroscopy
Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism - pp2857 - 2864
Masao Ota, Hirofumi Fukushima, Jerzy K Kulski & Hidetoshi Inoko
Published online: 08 November 2007 | doi:10.1038/nprot.2007.407
Abstract - Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism | Full Text - Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism | PDF (253 KB) - Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting - pp2865 - 2874
Hiroyuki Konishi, Josh Lauring, Joseph P Garay, Bedri Karakas, Abde M Abukhdeir, John P Gustin, Yuko Konishi & Ben H Park
Published online: 08 November 2007 | doi:10.1038/nprot.2007.409
Abstract - A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting | Full Text - A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting | PDF (376 KB) - A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting | Supplementary information
Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing - pp2875 - 2880
Yi Xiao, Rebecca Y Lai & Kevin W Plaxco
Published online: 08 November 2007 | doi:10.1038/nprot.2007.413
Abstract - Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing | Full Text - Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing | PDF (288 KB) - Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing
Pd-catalyzed amination reactions of aryl halides using bulky biarylmonophosphine ligands - pp2881 - 2887
Ryan A Altman, Brett P Fors & Stephen L Buchwald
Published online: 08 November 2007 | doi:10.1038/nprot.2007.414
Abstract - Pd-catalyzed amination reactions of aryl halides using bulky biarylmonophosphine ligands | Full Text - Pd-catalyzed amination reactions of aryl halides using bulky biarylmonophosphine ligands | PDF (304 KB) - Pd-catalyzed amination reactions of aryl halides using bulky biarylmonophosphine ligands
A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA - pp2888 - 2895
Jiong Zhang, Shiping Song, Lihua Wang, Dun Pan & Chunhai Fan
Published online: 08 November 2007 | doi:10.1038/nprot.2007.419
Abstract - A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA | Full Text - A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA | PDF (298 KB) - A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA
Automated structure determination of proteins with the SAIL-FLYA NMR method - pp2896 - 2902
Mitsuhiro Takeda, Teppei Ikeya, Peter Güntert & Masatsune Kainosho
Published online: 08 November 2007 | doi:10.1038/nprot.2007.423
Abstract - Automated structure determination of proteins with the SAIL-FLYA NMR method | Full Text - Automated structure determination of proteins with the SAIL-FLYA NMR method | PDF (437 KB) - Automated structure determination of proteins with the SAIL-FLYA NMR method
Preparation of arylzinc reagents and their use in the rhodium-catalyzed asymmetric 1,4-addition for the synthesis of 2-aryl-4-piperidones - pp2903 - 2909
Ryo Shintani & Tamio Hayashi
Published online: 08 November 2007 | doi:10.1038/nprot.2007.424
Abstract - Preparation of arylzinc reagents and their use in the rhodium-catalyzed asymmetric 1,4-addition for the synthesis of 2-aryl-4-piperidones | Full Text - Preparation of arylzinc reagents and their use in the rhodium-catalyzed asymmetric 1,4-addition for the synthesis of 2-aryl-4-piperidones | PDF (279 KB) - Preparation of arylzinc reagents and their use in the rhodium-catalyzed asymmetric 1,4-addition for the synthesis of 2-aryl-4-piperidones
An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome - pp2910 - 2917
Ronan C O'Malley, Jose M Alonso, Christopher J Kim, Thomas J Leisse & Joseph R Ecker
Published online: 08 November 2007 | doi:10.1038/nprot.2007.425
Abstract - An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the : Arabidopsis: genome | Full Text - An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome | PDF (265 KB) - An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome
The zebrafish/tumor xenograft angiogenesis assay - pp2918 - 2923
Stefania Nicoli & Marco Presta
Published online: 08 November 2007 | doi:10.1038/nprot.2007.412
Abstract - The zebrafish/tumor xenograft angiogenesis assay | Full Text - The zebrafish/tumor xenograft angiogenesis assay | PDF (332 KB) - The zebrafish/tumor xenograft angiogenesis assay
A manual method for the purification of fluorescently labeled neurons from the mammalian brain - pp2924 - 2929
Chris M Hempel, Ken Sugino & Sacha B Nelson
Published online: 08 November 2007 | doi:10.1038/nprot.2007.416
Abstract - A manual method for the purification of fluorescently labeled neurons from the mammalian brain | Full Text - A manual method for the purification of fluorescently labeled neurons from the mammalian brain | PDF (298 KB) - A manual method for the purification of fluorescently labeled neurons from the mammalian brain
Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation - pp2930 - 2944
Scott T Laughlin & Carolyn R Bertozzi
Published online: 15 November 2007 | doi:10.1038/nprot.2007.422
Abstract - Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation | Full Text - Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation | PDF (275 KB) - Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems - pp2945 - 2957
Daniel Schwarz, Friederike Junge, Florian Durst, Nadine Frölich, Birgit Schneider, Sina Reckel, Solmaz Sobhanifar, Volker Dötsch & Frank Bernhard
Published online: 15 November 2007 | doi:10.1038/nprot.2007.426
Abstract - Preparative scale expression of membrane proteins in : Escherichia coli: -based continuous exchange cell-free systems | Full Text - Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems | PDF (441 KB) - Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures - pp2958 - 2974
Sarah E Pierce, Ron W Davis, Corey Nislow & Guri Giaever
Published online: 15 November 2007 | doi:10.1038/nprot.2007.427
Abstract - Genome-wide analysis of barcoded : Saccharomyces cerevisiae: gene-deletion mutants in pooled cultures | Full Text - Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures | PDF (2,877 KB) - Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures | Supplementary information
Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR - pp2975 - 2986
Donald A Yergeau, Emin Kuliyev & Paul E Mead
Published online: 15 November 2007 | doi:10.1038/nprot.2007.428
Abstract - Injection-mediated transposon transgenesis in : Xenopus tropicalis: and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR | Full Text - Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR | PDF (361 KB) - Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR
Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation - pp2987 - 2995
William A Carlezon, Jr & Elena H Chartoff
Published online: 15 November 2007 | doi:10.1038/nprot.2007.441
Abstract - Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation | Full Text - Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation | PDF (247 KB) - Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation | Supplementary information
Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe - pp2996 - 3000
Juraj Gregan, Chao Zhang, Cornelia Rumpf, Lubos Cipak, Zhang Li, Pelin Uluocak, Kim Nasmyth & Kevan M Shokat
Published online: 15 November 2007 | doi:10.1038/nprot.2007.447
Abstract - Construction of conditional analog-sensitive kinase alleles in the fission yeast : Schizosaccharomyces: pombe | Full Text - Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe | PDF (199 KB) - Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe | Supplementary information
Selection of human antibody fragments by phage display - pp3001 - 3008
Carol M Y Lee, Niccolo Iorno, Frederic Sierro & Daniel Christ
Published online: 15 November 2007 | doi:10.1038/nprot.2007.448
Abstract - Selection of human antibody fragments by phage display | Full Text - Selection of human antibody fragments by phage display | PDF (318 KB) - Selection of human antibody fragments by phage display
Preparation of intact yeast artificial chromosome DNA for transgenesis of mice - pp3009 - 3015
Kenneth R Peterson
Published online: 15 November 2007 | doi:10.1038/nprot.2007.449
Abstract - Preparation of intact yeast artificial chromosome DNA for transgenesis of mice | Full Text - Preparation of intact yeast artificial chromosome DNA for transgenesis of mice | PDF (229 KB) - Preparation of intact yeast artificial chromosome DNA for transgenesis of mice
A model of MPTP-induced Parkinson's disease in the goldfish - pp3016 - 3021
Orly Weinreb & Moussa B H Youdim
Published online: 15 November 2007 | doi:10.1038/nprot.2007.393
Abstract - A model of MPTP-induced Parkinson's disease in the goldfish | Full Text - A model of MPTP-induced Parkinson's disease in the goldfish | PDF (329 KB) - A model of MPTP-induced Parkinson's disease in the goldfish
Designing and optimizing comparative anchor primers for comparative gene mapping and phylogenetic inference - pp3022 - 3030
William J Murphy & Stephen J O'Brien
Published online: 15 November 2007 | doi:10.1038/nprot.2007.429
Abstract - Designing and optimizing comparative anchor primers for comparative gene mapping and phylogenetic inference | Full Text - Designing and optimizing comparative anchor primers for comparative gene mapping and phylogenetic inference | PDF (342 KB) - Designing and optimizing comparative anchor primers for comparative gene mapping and phylogenetic inference | Supplementary information
