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Nature protocols

Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue pp2307 - 2311

Benno Weigmann, Ingrid Tubbe, Daniel Seidel, Alex Nicolaev, Christoph Becker & Markus F Neurath

Published online: 20 September 2007 | doi:10.1038/nprot.2007.315

See also: Protocol by Christianson & Gebhart


Synthesis of lactones using substituted benzoic anhydride as a coupling reagent pp2312 - 2317

Isamu Shiina, Hiroki Fukui & Akane Sasaki

Published online: 20 September 2007 | doi:10.1038/nprot.2007.316


Identification of enzymes and activity from two-dimensional gel electrophoresis pp2318 - 2324

Leila Afjehi-Sadat & Gert Lubec

Published online: 20 September 2007 | doi:10.1038/nprot.2007.317



Bicelle samples for solid-state NMR of membrane proteins pp2332 - 2338

Anna A De Angelis & Stanley J Opella

Published online: 20 September 2007 | doi:10.1038/nprot.2007.329


Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing pp2339 - 2344

Min Hu, Aaron R Jex, Bronwyn E Campbell & Robin B Gasser

Published online: 20 September 2007 | doi:10.1038/nprot.2007.358


Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia pp2345 - 2347

Ricardo J Komotar, Grace H Kim, Michael E Sughrue, Marc L Otten, Michal A Rynkowski, Christopher P Kellner, David K Hahn, Maxwell B Merkow, Matthew C Garrett, Robert M Starke & E Sander Connolly

Published online: 20 September 2007 | doi:10.1038/nprot.2007.359

See also: Protocol by Kalueff et al.



Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe pp2354 - 2365

Peter Z Qin, Ian S Haworth, Qi Cai, Ana K Kusnetzow, Gian Paola G Grant, Eric A Price, Glenna Z Sowa, Anna Popova, Bruno Herreros & Honghang He

Published online: 27 September 2007 | doi:10.1038/nprot.2007.308


Integration of biological networks and gene expression data using Cytoscape pp2366 - 2382

Melissa S Cline, Michael Smoot, Ethan Cerami, Allan Kuchinsky, Nerius Landys, Chris Workman, Rowan Christmas, Iliana Avila-Campilo, Michael Creech, Benjamin Gross, Kristina Hanspers, Ruth Isserlin, Ryan Kelley, Sarah Killcoyne, Samad Lotia, Steven Maere, John Morris, Keiichiro Ono, Vuk Pavlovic, Alexander R Pico, Aditya Vailaya, Peng-Liang Wang, Annette Adler, Bruce R Conklin, Leroy Hood, Martin Kuiper, Chris Sander, Ilya Schmulevich, Benno Schwikowski, Guy J Warner, Trey Ideker & Gary D Bader

Published online: 27 September 2007 | doi:10.1038/nprot.2007.324



Generation of magnetic nonviral gene transfer agents and magnetofection in vitro pp2391 - 2411

Olga Mykhaylyk, Yolanda Sánchez Antequera, Dialekti Vlaskou & Christian Plank

Published online: 27 September 2007 | doi:10.1038/nprot.2007.352


Preparation, application and testing of permanent antibacterial and antiviral coatings pp2412 - 2417

Jayanta Haldar, Alisha K Weight & Alexander M Klibanov

Published online: 27 September 2007 | doi:10.1038/nprot.2007.353



Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing pp2429 - 2438

John E Rice, J Aquiles Sanchez, Kenneth E Pierce, Arthur H Reis, Jr, Adam Osborne & Lawrence J Wangh

Published online: 27 September 2007 | doi:10.1038/nprot.2007.362




A membrane capture assay for lipid kinase activity pp2459 - 2466

Zachary A Knight, Morri E Feldman, Andras Balla, Tamas Balla & Kevan M Shokat

Published online: 04 October 2007 | doi:10.1038/nprot.2007.361


A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging pp2467 - 2473

Mohit Prasad, Anna C-C Jang, Michelle Starz-Gaiano, Mariana Melani & Denise J Montell

Published online: 04 October 2007 | doi:10.1038/nprot.2007.363



Cryosectioning and immunolabeling pp2480 - 2491

Jan W Slot & Hans J Geuze

Published online: 04 October 2007 | doi:10.1038/nprot.2007.365

See also: Protocol by Graham & Orenstein


Designing candidate gene and genome-wide case–control association studies pp2492 - 2501

Krina T Zondervan & Lon R Cardon

Published online: 04 October 2007 | doi:10.1038/nprot.2007.366


Qualitative PCR–ELISA protocol for the detection and typing of viral genomes pp2502 - 2510

Monica Musiani, Simona Venturoli, Giorgio Gallinella & Marialuisa Zerbini

Published online: 11 October 2007 | doi:10.1038/nprot.2007.311

See also: Protocol by Musiani et al.


Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes pp2511 - 2519

Monica Musiani, Giorgio Gallinella, Simona Venturoli & Marialuisa Zerbini

Published online: 11 October 2007 | doi:10.1038/nprot.2007.312

See also: Protocol by Musiani et al.


Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification pp2520 - 2528

Asli N Silahtaroglu, Dorrit Nolting, Lars Dyrskjøt, Eugene Berezikov, Morten Møller, Niels Tommerup & Sakari Kauppinen

Published online: 11 October 2007 | doi:10.1038/nprot.2007.313


Frontal affinity chromatography: sugar–protein interactions pp2529 - 2537

Hiroaki Tateno, Sachiko Nakamura-Tsuruta & Jun Hirabayashi

Published online: 11 October 2007 | doi:10.1038/nprot.2007.357


Analyzing grooming microstructure in neurobehavioral experiments pp2538 - 2544

Allan V Kalueff, J Wayne Aldridge, Justin L LaPorte, Dennis L Murphy & Pentti Tuohimaa

Published online: 11 October 2007 | doi:10.1038/nprot.2007.367

See also: Protocol by Komotar et al.


The mouse cornea micropocket angiogenesis assay pp2545 - 2550

Michael S Rogers, Amy E Birsner & Robert J D'Amato

Published online: 11 October 2007 | doi:10.1038/nprot.2007.368


Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction pp2551 - 2567

Linda W van Laake, Robert Passier, Jantine Monshouwer-Kloots, Marcel G Nederhoff, Dorien Ward-van Oostwaard, Loren J Field, Cees J van Echteld, Pieter A Doevendans & Christine L Mummery

Published online: 11 October 2007 | doi:10.1038/nprot.2007.371


Asymmetric aldol reaction using boron enolates pp2568 - 2573

Katie M Cergol & Mark J Coster

Published online: 11 October 2007 | doi:10.1038/nprot.2007.372


Production of a site- and phosphorylation state-specific antibody pp2574 - 2581

Hidemasa Goto & Masaki Inagaki

Published online: 18 October 2007 | doi:10.1038/nprot.2007.374


Preparation of iron oxide-based calcium sensors for MRI pp2582 - 2589

Tatjana Atanasijevic & Alan Jasanoff

Published online: 18 October 2007 | doi:10.1038/nprot.2007.377



Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization pp2601 - 2607

Jared T Hammill, Shigeki Miyake-Stoner, Jennifer L Hazen, Jennifer C Jackson & Ryan A Mehl

Published online: 18 October 2007 | doi:10.1038/nprot.2007.379


DMS footprinting of structured RNAs and RNA–protein complexes pp2608 - 2623

Pilar Tijerina, Sabine Mohr & Rick Russell

Published online: 18 October 2007 | doi:10.1038/nprot.2007.380


Assessment of colon sensitivity by luminal distension in mice pp2624 - 2631

Julie A Christianson & Gerald F Gebhart

Published online: 18 October 2007 | doi:10.1038/nprot.2007.392

See also: Protocol by Weigmann et al.



Nanostructure analysis using spatially modulated illumination microscopy pp2640 - 2646

David Baddeley, Claudia Batram, Yanina Weiland, Christoph Cremer & Udo J Birk

Published online: 18 October 2007 | doi:10.1038/nprot.2007.399


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