Latest Protocols
Volume 2 No 10
- Archive
In order to view all protocols, including both Nature Protocols and Protocols Network protocols, please browse by category.
To facilitate archiving and citing, Nature Protocols are allocated to publication months and assigned unique page numbers.
About the cover
Nature protocols
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue - pp2307 - 2311
Benno Weigmann, Ingrid Tubbe, Daniel Seidel, Alex Nicolaev, Christoph Becker & Markus F Neurath
Published online: 20 September 2007 | doi:10.1038/nprot.2007.315
Abstract - | Full Text - Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue | PDF (334 KB) - Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue
Synthesis of lactones using substituted benzoic anhydride as a coupling reagent - pp2312 - 2317
Isamu Shiina, Hiroki Fukui & Akane Sasaki
Published online: 20 September 2007 | doi:10.1038/nprot.2007.316
Abstract - | Full Text - Synthesis of lactones using substituted benzoic anhydride as a coupling reagent | PDF (280 KB) - Synthesis of lactones using substituted benzoic anhydride as a coupling reagent
Identification of enzymes and activity from two-dimensional gel electrophoresis - pp2318 - 2324
Leila Afjehi-Sadat & Gert Lubec
Published online: 20 September 2007 | doi:10.1038/nprot.2007.317
Abstract - | Full Text - Identification of enzymes and activity from two-dimensional gel electrophoresis | PDF (263 KB) - Identification of enzymes and activity from two-dimensional gel electrophoresis | Supplementary information
Creating transgenic Drosophila by microinjecting the site-specific 
C31 integrase mRNA and a transgene-containing donor plasmid - pp2325 - 2331
Matthew P Fish, Amy C Groth, Michele P Calos & Roel Nusse
Published online: 20 September 2007 | doi:10.1038/nprot.2007.328
Abstract - | Full Text - Creating transgenic Drosophila by microinjecting the site-specific 
C31 integrase mRNA and a transgene-containing donor plasmid | PDF (240 KB) - Creating transgenic Drosophila by microinjecting the site-specific C31 integrase mRNA and a transgene-containing donor plasmid
Bicelle samples for solid-state NMR of membrane proteins - pp2332 - 2338
Anna A De Angelis & Stanley J Opella
Published online: 20 September 2007 | doi:10.1038/nprot.2007.329
Abstract - | Full Text - Bicelle samples for solid-state NMR of membrane proteins | PDF (2,697 KB) - Bicelle samples for solid-state NMR of membrane proteins
Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing - pp2339 - 2344
Min Hu, Aaron R Jex, Bronwyn E Campbell & Robin B Gasser
Published online: 20 September 2007 | doi:10.1038/nprot.2007.358
Abstract - | Full Text - Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing | PDF (397 KB) - Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing
Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia - pp2345 - 2347
Ricardo J Komotar, Grace H Kim, Michael E Sughrue, Marc L Otten, Michal A Rynkowski, Christopher P Kellner, David K Hahn, Maxwell B Merkow, Matthew C Garrett, Robert M Starke & E Sander Connolly
Published online: 20 September 2007 | doi:10.1038/nprot.2007.359
Abstract - | Full Text - Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia | PDF (153 KB) - Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia
Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells - pp2348 - 2353
Yansong Miao & Liwen Jiang
Published online: 20 September 2007 | doi:10.1038/nprot.2007.360
Abstract - | Full Text - Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells | PDF (299 KB) - Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells
Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe - pp2354 - 2365
Peter Z Qin, Ian S Haworth, Qi Cai, Ana K Kusnetzow, Gian Paola G Grant, Eric A Price, Glenna Z Sowa, Anna Popova, Bruno Herreros & Honghang He
Published online: 27 September 2007 | doi:10.1038/nprot.2007.308
Abstract - | Full Text - Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe | PDF (440 KB) - Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe
Integration of biological networks and gene expression data using Cytoscape - pp2366 - 2382
Melissa S Cline, Michael Smoot, Ethan Cerami, Allan Kuchinsky, Nerius Landys, Chris Workman, Rowan Christmas, Iliana Avila-Campilo, Michael Creech, Benjamin Gross, Kristina Hanspers, Ruth Isserlin, Ryan Kelley, Sarah Killcoyne, Samad Lotia, Steven Maere, John Morris, Keiichiro Ono, Vuk Pavlovic, Alexander R Pico, Aditya Vailaya, Peng-Liang Wang, Annette Adler, Bruce R Conklin, Leroy Hood, Martin Kuiper, Chris Sander, Ilya Schmulevich, Benno Schwikowski, Guy J Warner, Trey Ideker & Gary D Bader
Published online: 27 September 2007 | doi:10.1038/nprot.2007.324
Abstract - | Full Text - Integration of biological networks and gene expression data using Cytoscape | PDF (852 KB) - Integration of biological networks and gene expression data using Cytoscape | Supplementary information
Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging - pp2383 - 2390
Olivier Carion, Benoît Mahler, Thomas Pons & Benoit Dubertret
Published online: 27 September 2007 | doi:10.1038/nprot.2007.351
Abstract - | Full Text - Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging | PDF (513 KB) - Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging
Generation of magnetic nonviral gene transfer agents and magnetofection in vitro - pp2391 - 2411
Olga Mykhaylyk, Yolanda Sánchez Antequera, Dialekti Vlaskou & Christian Plank
Published online: 27 September 2007 | doi:10.1038/nprot.2007.352
Abstract - | Full Text - Generation of magnetic nonviral gene transfer agents and magnetofection in vitro | PDF (714 KB) - Generation of magnetic nonviral gene transfer agents and magnetofection in vitro
Preparation, application and testing of permanent antibacterial and antiviral coatings - pp2412 - 2417
Jayanta Haldar, Alisha K Weight & Alexander M Klibanov
Published online: 27 September 2007 | doi:10.1038/nprot.2007.353
Abstract - | Full Text - Preparation, application and testing of permanent antibacterial and antiviral coatings | PDF (334 KB) - Preparation, application and testing of permanent antibacterial and antiviral coatings
Patent searches as a complement to literature searches in the life sciences—a 'how-to' tutorial - pp2418 - 2428
Frank Seeber
Published online: 27 September 2007 | doi:10.1038/nprot.2007.355
Abstract - | Full Text - Patent searches as a complement to literature searches in the life sciences—a 'how-to' tutorial | PDF (1,230 KB) - Patent searches as a complement to literature searches in the life sciences—a 'how-to' tutorial
Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing - pp2429 - 2438
John E Rice, J Aquiles Sanchez, Kenneth E Pierce, Arthur H Reis, Jr, Adam Osborne & Lawrence J Wangh
Published online: 27 September 2007 | doi:10.1038/nprot.2007.362
Abstract - | Full Text - Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing | PDF (486 KB) - Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing
Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research - pp2439 - 2450
Lesley Graham & Jan Marc Orenstein
Published online: 04 October 2007 | doi:10.1038/nprot.2007.304
Abstract - | Full Text - Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research | PDF (1,187 KB) - Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research
Mosher ester analysis for the determination of absolute configuration of stereogenic (chiral) carbinol carbons - pp2451 - 2458
Thomas R Hoye, Christopher S Jeffrey & Feng Shao
Published online: 04 October 2007 | doi:10.1038/nprot.2007.354
Abstract - | Full Text - Mosher ester analysis for the determination of absolute configuration of stereogenic (chiral) carbinol carbons | PDF (246 KB) - Mosher ester analysis for the determination of absolute configuration of stereogenic (chiral) carbinol carbons
A membrane capture assay for lipid kinase activity - pp2459 - 2466
Zachary A Knight, Morri E Feldman, Andras Balla, Tamas Balla & Kevan M Shokat
Published online: 04 October 2007 | doi:10.1038/nprot.2007.361
Abstract - | Full Text - A membrane capture assay for lipid kinase activity | PDF (298 KB) - A membrane capture assay for lipid kinase activity
A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging - pp2467 - 2473
Mohit Prasad, Anna C-C Jang, Michelle Starz-Gaiano, Mariana Melani & Denise J Montell
Published online: 04 October 2007 | doi:10.1038/nprot.2007.363
Abstract - | Full Text - A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging | PDF (381 KB) - A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging | Supplementary information
Cu-catalyzed Goldberg and Ullmann reactions of aryl halides using chelating N- and O-based ligands - pp2474 - 2479
Ryan A Altman & Stephen L Buchwald
Published online: 04 October 2007 | doi:10.1038/nprot.2007.364
Abstract - | Full Text - Cu-catalyzed Goldberg and Ullmann reactions of aryl halides using chelating N- and O-based ligands | PDF (307 KB) - Cu-catalyzed Goldberg and Ullmann reactions of aryl halides using chelating N- and O-based ligands
Cryosectioning and immunolabeling - pp2480 - 2491
Jan W Slot & Hans J Geuze
Published online: 04 October 2007 | doi:10.1038/nprot.2007.365
Abstract - | Full Text - Cryosectioning and immunolabeling | PDF (407 KB) - Cryosectioning and immunolabeling
Designing candidate gene and genome-wide case–control association studies - pp2492 - 2501
Krina T Zondervan & Lon R Cardon
Published online: 04 October 2007 | doi:10.1038/nprot.2007.366
Abstract - | Full Text - Designing candidate gene and genome-wide case–control association studies | PDF (241 KB) - Designing candidate gene and genome-wide case–control association studies | Supplementary information
Qualitative PCR–ELISA protocol for the detection and typing of viral genomes - pp2502 - 2510
Monica Musiani, Simona Venturoli, Giorgio Gallinella & Marialuisa Zerbini
Published online: 11 October 2007 | doi:10.1038/nprot.2007.311
Abstract - | Full Text - Qualitative PCR–ELISA protocol for the detection and typing of viral genomes | PDF (280 KB) - Qualitative PCR–ELISA protocol for the detection and typing of viral genomes
Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes - pp2511 - 2519
Monica Musiani, Giorgio Gallinella, Simona Venturoli & Marialuisa Zerbini
Published online: 11 October 2007 | doi:10.1038/nprot.2007.312
Abstract - | Full Text - Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes | PDF (244 KB) - Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes
Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification - pp2520 - 2528
Asli N Silahtaroglu, Dorrit Nolting, Lars Dyrskjøt, Eugene Berezikov, Morten Møller, Niels Tommerup & Sakari Kauppinen
Published online: 11 October 2007 | doi:10.1038/nprot.2007.313
Abstract - | Full Text - Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification | PDF (844 KB) - Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification
Frontal affinity chromatography: sugar–protein interactions - pp2529 - 2537
Hiroaki Tateno, Sachiko Nakamura-Tsuruta & Jun Hirabayashi
Published online: 11 October 2007 | doi:10.1038/nprot.2007.357
Abstract - | Full Text - Frontal affinity chromatography: sugar–protein interactions | PDF (582 KB) - Frontal affinity chromatography: sugar–protein interactions | Supplementary information
Analyzing grooming microstructure in neurobehavioral experiments - pp2538 - 2544
Allan V Kalueff, J Wayne Aldridge, Justin L LaPorte, Dennis L Murphy & Pentti Tuohimaa
Published online: 11 October 2007 | doi:10.1038/nprot.2007.367
Abstract - | Full Text - Analyzing grooming microstructure in neurobehavioral experiments | PDF (246 KB) - Analyzing grooming microstructure in neurobehavioral experiments | Supplementary information
The mouse cornea micropocket angiogenesis assay - pp2545 - 2550
Michael S Rogers, Amy E Birsner & Robert J D'Amato
Published online: 11 October 2007 | doi:10.1038/nprot.2007.368
Abstract - | Full Text - The mouse cornea micropocket angiogenesis assay | PDF (220 KB) - The mouse cornea micropocket angiogenesis assay | Supplementary information
Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction - pp2551 - 2567
Linda W van Laake, Robert Passier, Jantine Monshouwer-Kloots, Marcel G Nederhoff, Dorien Ward-van Oostwaard, Loren J Field, Cees J van Echteld, Pieter A Doevendans & Christine L Mummery
Published online: 11 October 2007 | doi:10.1038/nprot.2007.371
Abstract - | Full Text - Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction | PDF (694 KB) - Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction
Asymmetric aldol reaction using boron enolates - pp2568 - 2573
Katie M Cergol & Mark J Coster
Published online: 11 October 2007 | doi:10.1038/nprot.2007.372
Abstract - | Full Text - Asymmetric aldol reaction using boron enolates | PDF (265 KB) - Asymmetric aldol reaction using boron enolates
Production of a site- and phosphorylation state-specific antibody - pp2574 - 2581
Hidemasa Goto & Masaki Inagaki
Published online: 18 October 2007 | doi:10.1038/nprot.2007.374
Abstract - | Full Text - Production of a site- and phosphorylation state-specific antibody | PDF (386 KB) - Production of a site- and phosphorylation state-specific antibody
Preparation of iron oxide-based calcium sensors for MRI - pp2582 - 2589
Tatjana Atanasijevic & Alan Jasanoff
Published online: 18 October 2007 | doi:10.1038/nprot.2007.377
Abstract - | Full Text - Preparation of iron oxide-based calcium sensors for MRI | PDF (250 KB) - Preparation of iron oxide-based calcium sensors for MRI
Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells - pp2590 - 2600
T Ashton Cropp, J Christopher Anderson & Jason W Chin
Published online: 18 October 2007 | doi:10.1038/nprot.2007.378
Abstract - | Full Text - Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells | PDF (310 KB) - Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells
Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization - pp2601 - 2607
Jared T Hammill, Shigeki Miyake-Stoner, Jennifer L Hazen, Jennifer C Jackson & Ryan A Mehl
Published online: 18 October 2007 | doi:10.1038/nprot.2007.379
Abstract - | Full Text - Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization | PDF (240 KB) - Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization
DMS footprinting of structured RNAs and RNA–protein complexes - pp2608 - 2623
Pilar Tijerina, Sabine Mohr & Rick Russell
Published online: 18 October 2007 | doi:10.1038/nprot.2007.380
Abstract - | Full Text - DMS footprinting of structured RNAs and RNA–protein complexes | PDF (610 KB) - DMS footprinting of structured RNAs and RNA–protein complexes
Assessment of colon sensitivity by luminal distension in mice - pp2624 - 2631
Julie A Christianson & Gerald F Gebhart
Published online: 18 October 2007 | doi:10.1038/nprot.2007.392
Abstract - | Full Text - Assessment of colon sensitivity by luminal distension in mice | PDF (420 KB) - Assessment of colon sensitivity by luminal distension in mice
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli - pp2632 - 2639
Ario de Marco
Published online: 18 October 2007 | doi:10.1038/nprot.2007.400
Abstract - | Full Text - Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli | PDF (251 KB) - Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli
Nanostructure analysis using spatially modulated illumination microscopy - pp2640 - 2646
David Baddeley, Claudia Batram, Yanina Weiland, Christoph Cremer & Udo J Birk
Published online: 18 October 2007 | doi:10.1038/nprot.2007.399
Abstract - | Full Text - Nanostructure analysis using spatially modulated illumination microscopy | PDF (254 KB) - Nanostructure analysis using spatially modulated illumination microscopy | Supplementary information
