Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method - pp1 - 4

R Daniel Gietz & Robert H Schiestl

Published online: 31 January 2007 | doi:10.1038/nprot.2007.17

Abstract - Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method | Full Text - Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method | PDF (124 KB) - Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method

See also: Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl

Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method - pp5 - 8

R Daniel Gietz & Robert H Schiestl

Published online: 31 January 2007 | doi:10.1038/nprot.2007.16

Abstract - Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method | Full Text - Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method | PDF (115 KB) - Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method

See also: Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl

Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held - pp9 - 22

Brian K Hoffpauir, Brian A Pope & George A Spirou

Published online: 31 January 2007 | doi:10.1038/nprot.2007.9

Abstract - Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held | Full Text - Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held | PDF (655 KB) - Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held | Supplementary information

A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds - pp23 - 30

Peng Yu, Bo Liu & Thomas Kodadek

Published online: 31 January 2007 | doi:10.1038/nprot.2007.1

Abstract - A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds | Full Text - A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds | PDF (267 KB) - A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method - pp31 - 34

R Daniel Gietz & Robert H Schiestl

Published online: 31 January 2007 | doi:10.1038/nprot.2007.13

Abstract - High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method | Full Text - High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method | PDF (123 KB) - High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

See also: Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl

Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method - pp35 - 37

R Daniel Gietz & Robert H Schiestl

Published online: 31 January 2007 | doi:10.1038/nprot.2007.14

Abstract - Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method | Full Text - Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method | PDF (110 KB) - Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method

See also: Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl

Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method - pp38 - 41

R Daniel Gietz & Robert H Schiestl

Published online: 31 January 2007 | doi:10.1038/nprot.2007.15

Abstract - Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method | Full Text - Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method | PDF (123 KB) - Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

See also: Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl | Protocol by Gietz & Schiestl

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes - pp42 - 49

Christine B Karim, Zhiwen Zhang & David D Thomas

Published online: 31 January 2007 | doi:10.1038/nprot.2007.2

Abstract - Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes | Full Text - Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes | PDF (248 KB) - Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes

Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping - pp50 - 58

Jin Li & G Mike Makrigiorgos

Published online: 22 February 2007 | doi:10.1038/nprot.2007.11

Abstract - Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping | Full Text - Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping | PDF (215 KB) - Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping

Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis - pp59 - 66

Jesse Montgomery, Carl T Wittwer, Robert Palais & Luming Zhou

Published online: 22 February 2007 | doi:10.1038/nprot.2007.10

Abstract - Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis | Full Text - Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis | PDF (376 KB) - Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis

In vivo models of proliferative vitreoretinopathy - pp67 - 77

Rajat N Agrawal, Shikun He, Christine Spee, Jing Z Cui, Stephen J Ryan & David R Hinton

Published online: 22 February 2007 | doi:10.1038/nprot.2007.4

Abstract - In vivo: models of proliferative vitreoretinopathy | Full Text - In vivo models of proliferative vitreoretinopathy | PDF (384 KB) - In vivo models of proliferative vitreoretinopathy

Photochemical determination of different DNA structures - pp78 - 87

Yan Xu, Ryu Tashiro & Hiroshi Sugiyama

Published online: 08 February 2007 | doi:10.1038/nprot.2006.467

Abstract - Photochemical determination of different DNA structures | Full Text - Photochemical determination of different DNA structures | PDF (678 KB) - Photochemical determination of different DNA structures

BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment - pp88 - 98

Glyn Jenkins & Robert Hasterok

Published online: 08 February 2007 | doi:10.1038/nprot.2006.490

Abstract - BAC 'landing' on chromosomes of : Brachypodium distachyon: for comparative genome alignment | Full Text - BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment | PDF (382 KB) - BAC 'landing' on chromosomes of Brachypodium distachyon for comparative genome alignment | Supplementary information

Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol - pp99 - 104

Kirsten Haastert, Christina Mauritz, Sukhada Chaturvedi & Claudia Grothe

Published online: 08 February 2007 | doi:10.1038/nprot.2006.486

Abstract - Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol | Full Text - Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol | PDF (337 KB) - Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol

A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators - pp105 - 112

Joachim H Maxeiner, Roman Karwot, Michael Hausding, Kerstin A Sauer, Petra Scholtes & Susetta Finotto

Published online: 22 February 2007 | doi:10.1038/nprot.2007.8

Abstract - A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators | Full Text - A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators | PDF (394 KB) - A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators

L-Proline-catalyzed enantioselective one-pot cross-Mannich reaction of aldehydes - pp113 - 118

Yujiro Hayashi, Tatsuya Urushima, Wataru Tsuboi & Mitsuru Shoji

Published online: 15 February 2007 | doi:10.1038/nprot.2006.472

Abstract - L: -Proline-catalyzed enantioselective one-pot cross-Mannich reaction of aldehydes | Full Text - L-Proline-catalyzed enantioselective one-pot cross-Mannich reaction of aldehydes | PDF (167 KB) - L-Proline-catalyzed enantioselective one-pot cross-Mannich reaction of aldehydes | Supplementary information

Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors - pp119 - 130

Oscar Yanes, Josep Villanueva, Enrique Querol & Francesc X Aviles

Published online: 15 February 2007 | doi:10.1038/nprot.2006.487

Abstract - Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors | Full Text - Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors | PDF (549 KB) - Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors

A primary culture technique of adult retina for regeneration studies on adult CNS neurons - pp131 - 140

Alexandra Kretz, Julia K Marticke, Caroline J Happold, Christian Schmeer & Stefan Isenmann

Published online: 22 February 2007 | doi:10.1038/nprot.2007.12

Abstract - A primary culture technique of adult retina for regeneration studies on adult CNS neurons | Full Text - A primary culture technique of adult retina for regeneration studies on adult CNS neurons | PDF (371 KB) - A primary culture technique of adult retina for regeneration studies on adult CNS neurons

See also: Protocol by Aemissegger & Hilvert | Protocol by Malin et al.

Protocol for the MPTP mouse model of Parkinson's disease - pp141 - 151

Vernice Jackson-Lewis & Serge Przedborski

Published online: 15 February 2007 | doi:10.1038/nprot.2006.342

Abstract - Protocol for the MPTP mouse model of Parkinson's disease | Full Text - Protocol for the MPTP mouse model of Parkinson's disease | PDF (295 KB) - Protocol for the MPTP mouse model of Parkinson's disease | Supplementary information

Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity - pp152 - 160

Sacha A Malin, Brian M Davis & Derek C Molliver

Published online: 15 February 2007 | doi:10.1038/nprot.2006.461

Abstract - Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity | Full Text - Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity | PDF (308 KB) - Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity

See also: Protocol by Kretz et al. | Protocol by Aemissegger & Hilvert

Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides - pp161 - 167

Andreas Aemissegger & Donald Hilvert

Published online: 08 February 2007 | doi:10.1038/nprot.2006.488

Abstract - Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides | Full Text - Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides | PDF (182 KB) - Synthesis and application of an azobenzene amino acid as a light-switchable turn element in polypeptides

See also: Protocol by Kretz et al. | Protocol by Malin et al.

The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms - pp168 - 177

Runchun Jing, Viacheslav Bolshakov & Andrew J Flavell

Published online: 08 February 2007 | doi:10.1038/nprot.2006.408

Abstract - The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms | Full Text - The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms | PDF (377 KB) - The tagged microarray marker (TAM) method for high-throughput detection of single nucleotide and indel polymorphisms

Establishment of 3D organotypic cultures using human neonatal epidermal cells - pp178 - 186

Pradnya Gangatirkar, Sophie Paquet-Fifield, Amy Li, Ralph Rossi & Pritinder Kaur

Published online: 08 February 2007 | doi:10.1038/nprot.2006.448

Abstract - Establishment of 3D organotypic cultures using human neonatal epidermal cells | Full Text - Establishment of 3D organotypic cultures using human neonatal epidermal cells | PDF (311 KB) - Establishment of 3D organotypic cultures using human neonatal epidermal cells

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D - pp187 - 190

Ingrid Schmid, Christel Uittenbogaart & Beth D Jamieson

Published online: 15 February 2007 | doi:10.1038/nprot.2006.458

Abstract - Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D | Full Text - Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D | PDF (181 KB) - Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D

Purification of naturally occurring peptides by reversed-phase HPLC - pp191 - 197

J Michael Conlon

Published online: 08 February 2007 | doi:10.1038/nprot.2006.437

Abstract - Purification of naturally occurring peptides by reversed-phase HPLC | Full Text - Purification of naturally occurring peptides by reversed-phase HPLC | PDF (237 KB) - Purification of naturally occurring peptides by reversed-phase HPLC

Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase - pp198 - 202

Nikolay B Pestov & Jan Rydström

Published online: 08 February 2007 | doi:10.1038/nprot.2006.456

Abstract - Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase | Full Text - Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase | PDF (186 KB) - Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase

Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription - pp203 - 212

Hiroyuki Asanuma, Xingguo Liang, Hidenori Nishioka, Daijiro Matsunaga, Mingzhe Liu & Makoto Komiyama

Published online: 08 February 2007 | doi:10.1038/nprot.2006.465

Abstract - Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription | Full Text - Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription | PDF (430 KB) - Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription

Metal-chelate affinity precipitation of proteins using responsive polymers - pp213 - 220

Bo Mattiasson, Ashok Kumar, Alexander E Ivanov & Igor Y Galaev

Published online: 08 February 2007 | doi:10.1038/nprot.2006.440

Abstract - Metal-chelate affinity precipitation of proteins using responsive polymers | Full Text - Metal-chelate affinity precipitation of proteins using responsive polymers | PDF (1,342 KB) - Metal-chelate affinity precipitation of proteins using responsive polymers

Quantification of F₂-isoprostanes as a biomarker of oxidative stress - pp221 - 226

Ginger L Milne, Stephanie C Sanchez, Erik S Musiek & Jason D Morrow

Published online: 08 February 2007 | doi:10.1038/nprot.2006.375

Abstract - Quantification of F: 2: -isoprostanes as a biomarker of oxidative stress | Full Text - Quantification of F2-isoprostanes as a biomarker of oxidative stress | PDF (1,376 KB) - Quantification of F2-isoprostanes as a biomarker of oxidative stress

Synthesis and use of an in-solution ratiometric fluorescent viscosity sensor - pp227 - 236

Derek Fischer, Emmanuel A Theodorakis & Mark A Haidekker

Published online: 08 February 2007 | doi:10.1038/nprot.2006.455

Abstract - Synthesis and use of an in-solution ratiometric fluorescent viscosity sensor | Full Text - Synthesis and use of an in-solution ratiometric fluorescent viscosity sensor | PDF (238 KB) - Synthesis and use of an in-solution ratiometric fluorescent viscosity sensor

Heteroduplex analysis by capillary array electrophoresis for rapid mutation detection in large multiexon genes - pp237 - 246

Eladio Velasco, Mar Infante, Mercedes Durán, Lucía Pérez-Cabornero, David J Sanz, Eva Esteban-Cardeñosa & Cristina Miner

Published online: 15 February 2007 | doi:10.1038/nprot.2006.482

Abstract - Heteroduplex analysis by capillary array electrophoresis for rapid mutation detection in large multiexon genes | Full Text - Heteroduplex analysis by capillary array electrophoresis for rapid mutation detection in large multiexon genes | PDF (443 KB) - Heteroduplex analysis by capillary array electrophoresis for rapid mutation detection in large multiexon genes