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Protocol
Nature Protocols 2, 5–8 (1 January 2007) | doi:10.1038/nprot.2007.16
Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method
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Abstract
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2–3 h, once the yeast cells have been grown depending on the heat shock used.
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