Abstract
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2–3 h, once the yeast cells have been grown depending on the heat shock used.
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Change history
04 December 2008
In the version of this article initially published, in the Reagent Setup section on p. 6, the recipe for lithium acetate (1.0 M) called for 102 g of lithium acetate dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.
References
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Gietz, R., Schiestl, R. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 5–8 (2007). https://doi.org/10.1038/nprot.2007.16
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DOI: https://doi.org/10.1038/nprot.2007.16
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