Protocol abstract
Nature Protocols 2, - 5 - 8 (2007)
Published online: 31 January 2007 | doi:10.1038/nprot.2007.16
Subject Categories: Cell and tissue culture | Model organisms | Genetic modification | Microbiology and virology
Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method
R Daniel Gietz1 & Robert H Schiestl2
Abstract
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2–3 h, once the yeast cells have been grown depending on the heat shock used.
- Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
- Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, California 90095, USA.
Correspondence to: Robert H Schiestl2 e-mail: rschiestl@mednet.ucla.edu
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A-Z product listing
- 96-well replicator(Boekel Scientific Feasterville)
- 96-well round bottom microwell plate(Fisher Scientific Ltd.)
- Adenine hemisulfate(Sigma Chemical Co. Ltd.)
- Bacto Agar(Fisher Scientific Ltd.)
- Bacto peptone(Fisher Scientific Ltd.)
- Bacto yeast extract(Fisher Scientific Ltd.)