Protocol abstract
Nature Protocols 2, - 35 - 37 (2007)
Published online: 31 January 2007 | doi:10.1038/nprot.2007.14
Subject Categories: Cell and tissue culture | Model organisms | Genetic modification | Microbiology and virology
Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method
R Daniel Gietz1 & Robert H Schiestl2
Abstract
Here, we describe a quick and easy version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method can be performed when only a few transformants are needed. The procedure can take less than an hour, depending on the duration of the heat shock. It can be used to transform yeast cells from various stages of growth and storage. Cells can be transformed from freshly grown cells as well as cells stored on a plate at room temperature or in a refrigerator.
- Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
- Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, California 90095, USA.
Correspondence to: Robert H Schiestl2 e-mail: rschiestl@mednet.ucla.edu
nature-products
A-Z product listing
- 1.5 ml polypropylene microcentrifuge tubes(Fisher Scientific Ltd)
- 100
15 mm sterile disposable Petri plates(Fisher Scientific Ltd) - Adenine hemisulfate(Sigma Chemical Co. Ltd)
- Bacto Agar(Fisher Scientific Ltd)
- Bacto peptone(Fisher Scientific Ltd)
- Bacto yeast extract(Fisher Scientific Ltd)
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