Volume 14 Issue 12, December 2019

hPSCs are differentiated into anterior foregut endoderm and then undergo lineage specification into NKX2-1⁺ lung progenitor cells. Next, the progenitors undergo distal/alveolar differentiation to produce self-renewing alveolar epithelial type II cells.

The authors present a new protocol to quantitatively assess protein turnover in mice in vivo. The procedure includes instructions on the mouse diet for stable isotope labeling of amino acids in mammals (SILAM), sample preparation, mass spectrometry analysis and data processing.

This protocol provides instructions for the fabrication, assembly and operation of a microfluidic device used for chromatin immunoprecipitation followed by sequencing to profile histone modifications in low-input samples.

Surgical techniques are presented that facilitate decellularization of various mouse tissues. Immunostaining and microscopy of the resulting tissues is also presented using an extracellular matrix–specific antibody catalog.

PheCAP takes structured data and narrative notes from electronic medical records and enables patients with a particular clinical phenotype to be identified.

This protocol describes how to achieve high spatial resolution imaging of biological tissues using nanospray desorption electrospray ionization mass spectrometry

This protocol describes how to redesign, characterize, validate and apply genetically encoded dopamine sensors, such as those of the dLight1 family, to measure dopamine transients in cultured cells, neurons, acute brain slices and freely behaving, awake mice.

Transparent organs are obtained, with retained fluorescent protein signals, upon clearing by immersion in the appropriate CUBIC reagent. The positions of all the cells can be determined using the described software.

This protocol covers the preparation and use of artificial microRNA clusters for gene therapy. The in silico design of chimeric microRNA transgenes in which up to six natural microRNA hairpins are replaced by synthetic versions obviates the need for complex molecular cloning.