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A fluorescent image of a 3D human cortical spheroid (hCS) derived from induced pluripotent stem cells that was plated on a glass coverslip and immunostained with antibodies against MAP2 (neurons; green), GFAP (glial lineage cells; purple) and DAPI (nucleiI, blue).
This protocol describes the EasyPhos platform, a high-throughput and user-friendly workflow for reliable identification of phosphopeptides from small amounts (<200 μg) of sample.
This protocol describes TRACER, a 3D cell culture system that enables the assembly of heterogeneous model tumors or tissues that easily disassemble for rapid analysis of different cell populations from particular microenvironments.
Phage immunoprecipitation sequencing (PhIP-Seq) is a method for analyzing antibody-repertoire binding specificities. Phage-displayed oligonucleotide libraries encoding peptidomes are immunoprecipitated and analyzed by high-throughput DNA-Seq.
This protocol describes fit-free analysis of fluorescence lifetime imaging microscopy (FLIM) data using the phasor approach. Pixel-by-pixel decays are transformed to the phasor space, and then the clusters can be connected to the image by the reciprocity rules of the phasor plots.
This protocol describes focused ion beam–milling approaches for fabricating oriented single-nanocrystal atomic force microscopy probes, as well as how to measure direction-specific interaction forces between nanocrystals in solution and in vacuum.
This protocol describes how to configure targeted spinal cord stimulation using electromyographic recordings and real-time processing of gait kinematics to enable voluntary control of specific leg movements in rats and nonhuman primates.
Neural differentiation and self-organization of hPSCs are induced by culture in suspension. Neural spheroids are then differentiated into dorsal or ventral forebrain spheroids that can be combined to obtain functionally integrated human assembloids.
Improved and robust procedures for a cyclodextrin-mediated preparation of asymmetric large unilamellar vesicles of diverse lipid compositions and the characterization of their degree of asymmetry and individual leaflet compositions with NMR and GC.
This protocol describes how to use a fully defined, synthetic hydrogel to support the in vitro generation and culture of human organoids derived from pluripotent stem cells and the in vivo delivery of hydrogel-encapsulated organoids into mouse colon.