Volume 11 Issue 6, June 2016

Synthetic ion channels are used to study biological processes and develop sensing, drug delivery and antimicrobial systems. This protocol uses click chemistry to prepare lipophilic diguanosine derivatives that assemble into metal cation channels.

Gallium-68 is a generator-produced positron-emitting isotope. ⁶⁸Ga-labeled peptides are used for PET imaging. To prepare them, this protocol describes an efficient method for preconcentration and purification of ⁶⁸Ga.

This protocol from Bonora et al. describes three imaging techniques for examining mitochondrial permeability transition (mPT) in living cells.

This protocol for methylase-asssisted bisulfite sequencing (MAB-seq) and caMAB-seq allows the analysis of active DNA demethylation, directly mapping 5fC and 5caC at single-base resolution.

In-cell NMR provides detailed structural information about proteins in their native environment in living cells. This in-cell NMR protocol allows the study of protein folding and maturation in the presence of cofactors in cultured mammalian cells.

This protocol provides an easy-to-use, click-by-click guide for the analysis of longitudinal data from animals in SPSS. The authors use the MIXED procedure to deal with instances in which data are missing, by fitting linear models with general error covariance structures.

In this protocol from Bode and co-workers, peptide fragments containing N-terminal 5-oxaproline and a C-terminal α-ketoacids are prepared by solid-phase peptide synthesis. KAHA ligation results in the formation of medium-sized proteins.