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Pseudocolored mask image obtained after segmentation of DAPI-stained cell nuclei. Individual nuclear stencils are used to measure the integrated DAPI intensity, which is then used to determine the cell cycle stage of each individual cell in the population. Taken from the protocol by Roukos et al. doi: 10.1038/nprot.2015.016. Cover design by Jamel Wooten.
Chemical compositional information can be extracted from Raman and infrared microscopic images by MCR-ALS. The algorithm finds the spectral profiles of compounds contributing to each image pixel and their relative concentrations.
Zuo and Morris describe an approach to catalytic hydrogenation of prochiral ketones and imines to produce enantioenriched alcohols and amines that uses a more environmentally friendly iron-based catalyst instead of conventional Ru-based catalysts.
This protocol describes how to perform liver confocal intravital microscopy in mice, revealing the morphology and function of hepatocytes, endothelial cells and leukocytes in their native environment under physiological or pathological conditions.
Protein SUMOylation, which has a crucial role in the regulation of important cellular processes, is difficult to replicate using living systems in the lab. Boll et al. describe here the chemical synthesis of functional SUMO-peptide conjugates.
This article provides guidelines on how to perform a voxel-based gray matter asymmetry analysis, taking structural brain images from the initial data pre-processing through statistical analyses and the final interpretation of the significance maps.
This protocol uses retrograde pronase-collagenase perfusion of the liver and subsequent density-gradient centrifugation and optional flow-cytometric sorting to isolate hepatic stellate cells.
This protocol describes how to use mass-tag cell barcoding to label individual cell samples with unique combinatorial barcodes. The samples can then be pooled for processing and measurement on a mass cytometer.
This Protocol from the Misteli lab describes how to determine the cell cycle stage of each individual cell in a population by using fluorescence microscopy and automated image analysis software.
UbiCRest is a simple PAGE-based method that leverages the varying specificities of deubiquitinating enzymes (DUBs) to generate information about the composition and architecture of ubiquitin chains.