These authors contributed equally to this work.
- Ken Y Chan,
- Nicholas C Flytzanis &
- Bin Yang
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
- Jennifer B Treweek,
- Ken Y Chan,
- Nicholas C Flytzanis,
- Bin Yang,
- Benjamin E Deverman,
- Alon Greenbaum,
- Cheng Xiao,
- Mark S Ladinsky,
- Pamela J Bjorkman &
- Viviana Gradinaru
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
- Antti Lignell &
- Long Cai
Department of Computer Science, University of California, Irvine, California, USA.
- Charless C Fowlkes
J.B.T. and V.G. wrote the manuscript with input from all coauthors. J.B.T., K.Y.C., N.C.F., B.Y., B.E.D. and V.G. designed and performed experiments, analyzed the data and prepared figures. C.C.F. wrote the data analysis section, including associated figures and data analysis; C.X. assisted with tissue clearing and imaging for data sets in this section. A.G., A.L., L.C. and V.G. planned for and built the light sheet and collected and analyzed the associated data. M.S.L., P.J.B. and V.G. planned and performed TEM tissue processing and imaging and prepared the EM figure. V.G. supervised all aspects of the project. All authors edited and approved the manuscript.
Competing financial interests
The authors declare no competing financial interests.
Jennifer B Treweek
Ken Y Chan
Nicholas C Flytzanis
Benjamin E Deverman
Mark S Ladinsky
Pamela J Bjorkman
Charless C Fowlkes
Supplementary Figure 1: Effects of bis-acrylamide crosslinker on clearing time and swelling of PACT-cleared sections.Hover over figure to zoom
Supplementary Figure 2: Protein loss over the course of PACT clearing.Hover over figure to zoom
Supplementary Figure 3: PACT compatibility with histological staining.Hover over figure to zoom
Supplementary Figure 4: ePACT: a protocol for tissue clearing through expansion.Hover over figure to zoom
Supplementary Figure 5: Whole body PARS clearing with borate-buffered detergent.Hover over figure to zoom
Supplementary Figure 6: Small format antibodies for thick-tissue labeling.Hover over figure to zoom
Supplementary Figure 7: User interface elements for image analysis.Hover over figure to zoom