Video 1: Spot-counting analysis of hippocampal neurons in Thy1-YFP-H Tg mouse brain shown in Fig. 4b-f.

FromAdvanced CUBIC protocols for whole-brain and whole-body clearing and imaging

Journal name:
Nature Protocols
Volume:
10,
Pages:
1709–1727
Year published:
DOI:
doi:10.1038/nprot.2015.085
Published online
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Almost all cells are roughly detected as single spots in such a relatively dense region. The analysis was performed with Imaris software. Some of parameters are manually adjusted.

Additional data