Latest Protocols
Volume 1 No 6
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Nature protocols
Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions - pp2527 - 2535
Norma J Greenfield
Published online: 29 December 2006 | doi:10.1038/nprot.2006.204
Abstract - | Full Text - Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions | PDF (397 KB) - Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions | Supplementary information
See also: Protocol by Greenfield | Protocol by Greenfield | Protocol by Greenfield
A simple trogocytosis-based method to detect, quantify, characterize and purify antigen-specific live lymphocytes by flow cytometry, via their capture of membrane fragments from antigen-presenting cells - pp2536 - 2542
Sandrine Daubeuf, Anne-Laure Puaux, Etienne Joly & Denis Hudrisier
Published online: 29 December 2006 | doi:10.1038/nprot.2006.400
Abstract - | Full Text - A simple trogocytosis-based method to detect, quantify, characterize and purify antigen-specific live lymphocytes by flow cytometry, via their capture of membrane fragments from antigen-presenting cells | PDF (285 KB) - A simple trogocytosis-based method to detect, quantify, characterize and purify antigen-specific live lymphocytes by flow cytometry, via their capture of membrane fragments from antigen-presenting cells
Preparation and maintenance of single-cell micro-island cultures of basal forebrain neurons - pp2543 - 2550
Timothy G J Allen
Published online: 04 January 2007 | doi:10.1038/nprot.2006.394
Abstract - | Full Text - Preparation and maintenance of single-cell micro-island cultures of basal forebrain neurons | PDF (272 KB) - Preparation and maintenance of single-cell micro-island cultures of basal forebrain neurons
The mode-of-action by network identification (MNI) algorithm: a network biology approach for molecular target identification - pp2551 - 2554
Heming Xing & Timothy S Gardner
Published online: 29 December 2006 | doi:10.1038/nprot.2006.300
Abstract - | Full Text - The mode-of-action by network identification (MNI) algorithm: a network biology approach for molecular target identification | PDF (182 KB) - The mode-of-action by network identification (MNI) algorithm: a network biology approach for molecular target identification
5' end cDNA amplification using classic RACE - pp2555 - 2562
Elizabeth Scotto–Lavino, Guangwei Du & Michael A Frohman
Published online: 29 December 2006 | doi:10.1038/nprot.2006.480
Abstract - | Full Text - 5' end cDNA amplification using classic RACE | PDF (304 KB) - 5' end cDNA amplification using classic RACE
See also: Protocol by Scotto-Lavino et al. | Protocol by Scotto–Lavino et al.
Analysis of mitochondrial function using phosphorescent oxygen-sensitive probes - pp2563 - 2572
Yvonne Will, James Hynes, Vladimir I Ogurtsov & Dmitri B Papkovsky
Published online: 04 January 2007 | doi:10.1038/nprot.2006.351
Abstract - | Full Text - Analysis of mitochondrial function using phosphorescent oxygen-sensitive probes | PDF (303 KB) - Analysis of mitochondrial function using phosphorescent oxygen-sensitive probes
Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using Pyrosequencing™ - pp2573 - 2582
Catharina Lavebratt & Selim Sengul
Published online: 11 January 2007 | doi:10.1038/nprot.2006.442
Abstract - | Full Text - Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using Pyrosequencing™ | PDF (427 KB) - Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using Pyrosequencing™
See also: Protocol by Gasser et al. | Protocol by Chiou et al. | Protocol by Zhang et al.
A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila - pp2583 - 2589
Joy S Wu & Liqun Luo
Published online: 11 January 2007 | doi:10.1038/nprot.2006.320
Abstract - | Full Text - A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila | PDF (324 KB) - A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila
Preparation of substituted anilines from nitro compounds by using supported gold catalysts - pp2590 - 2595
Avelino Corma & Pedro Serna
Published online: 11 January 2007 | doi:10.1038/nprot.2006.441
Abstract - | Full Text - Preparation of substituted anilines from nitro compounds by using supported gold catalysts | PDF (217 KB) - Preparation of substituted anilines from nitro compounds by using supported gold catalysts
Total synthesis of multi-kilobase DNA sequences from oligonucleotides - pp2596 - 2603
Sarah J Reisinger, Kedar G Patel & Daniel V Santi
Published online: 11 January 2007 | doi:10.1038/nprot.2006.426
Abstract - | Full Text - Total synthesis of multi-kilobase DNA sequences from oligonucleotides | PDF (401 KB) - Total synthesis of multi-kilobase DNA sequences from oligonucleotides
Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations - pp2604 - 2612
Chiuan-Chian Chiou, Ji-Dung Luo & Tai-Long Chen
Published online: 11 January 2007 | doi:10.1038/nprot.2006.428
Abstract - | Full Text - Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations | PDF (323 KB) - Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations
See also: Protocol by Gasser et al. | Protocol by Zhang et al. | Protocol by Lavebratt & Sengul
Highly purified selective isolation of eosinophils from human peripheral blood by negative immunomagnetic selection - pp2613 - 2620
Nilda M Munoz & Alan R Leff
Published online: 11 January 2007 | doi:10.1038/nprot.2006.340
Abstract - | Full Text - Highly purified selective isolation of eosinophils from human peripheral blood by negative immunomagnetic selection | PDF (662 KB) - Highly purified selective isolation of eosinophils from human peripheral blood by negative immunomagnetic selection
Cell cycle synchronization of tobacco BY-2 cells - pp2621 - 2627
Fumi Kumagai-Sano, Tomomi Hayashi, Toshio Sano & Seiichiro Hasezawa
Published online: 11 January 2007 | doi:10.1038/nprot.2006.381
Abstract - | Full Text - Cell cycle synchronization of tobacco BY-2 cells | PDF (279 KB) - Cell cycle synchronization of tobacco BY-2 cells
Imaging the lateral diffusion of membrane molecules with quantum dots - pp2628 - 2634
Hiroko Bannai, Sabine Lévi, Claude Schweizer, Maxime Dahan & Antoine Triller
Published online: 11 January 2007 | doi:10.1038/nprot.2006.429
Abstract - | Full Text - Imaging the lateral diffusion of membrane molecules with quantum dots | PDF (304 KB) - Imaging the lateral diffusion of membrane molecules with quantum dots | Supplementary information
Immunofluorescent staining and imaging of the pupal and adult Drosophila visual system - pp2635 - 2642
Rhian F Walther & Franck Pichaud
Published online: 18 January 2007 | doi:10.1038/nprot.2006.379
Abstract - | Full Text - Immunofluorescent staining and imaging of the pupal and adult Drosophila visual system | PDF (477 KB) - Immunofluorescent staining and imaging of the pupal and adult Drosophila visual system
Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients - pp2643 - 2649
Trevor G Shepherd, Brigitte L Thériault, Elizabeth J Campbell & Mark W Nachtigal
Published online: 11 January 2007 | doi:10.1038/nprot.2006.328
Abstract - | Full Text - Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients | PDF (299 KB) - Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) - pp2650 - 2660
Shao-En Ong & Matthias Mann
Published online: 11 January 2007 | doi:10.1038/nprot.2006.427
Abstract - | Full Text - A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) | PDF (383 KB) - A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)
See also: Protocol by Shevchenko et al.
Advances in water treatment by adsorption technology - pp2661 - 2667
Imran Ali & V K Gupta
Published online: 11 January 2007 | doi:10.1038/nprot.2006.370
Abstract - | Full Text - Advances in water treatment by adsorption technology | PDF (243 KB) - Advances in water treatment by adsorption technology | Supplementary information
Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic tool - pp2668 - 2680
Fanrong Kong & Gwendolyn L Gilbert
Published online: 11 January 2007 | doi:10.1038/nprot.2006.404
Abstract - | Full Text - Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic tool | PDF (379 KB) - Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic tool | Supplementary information
Preparation of primary cultured mesenteric artery smooth muscle cells for fluorescent imaging and physiological studies - pp2681 - 2687
Vera A Golovina & Mordecai P Blaustein
Published online: 11 January 2007 | doi:10.1038/nprot.2006.425
Abstract - | Full Text - Preparation of primary cultured mesenteric artery smooth muscle cells for fluorescent imaging and physiological studies | PDF (268 KB) - Preparation of primary cultured mesenteric artery smooth muscle cells for fluorescent imaging and physiological studies
Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes - pp2688 - 2700
Anthony Simmons & Kristina Jantz
Published online: 11 January 2007 | doi:10.1038/nprot.2006.409
Abstract - | Full Text - Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes | PDF (625 KB) - Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes
Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy - pp2701 - 2709
Zach Serber, Philipp Selenko, Robert Hänsel, Sina Reckel, Frank Löhr, James E Ferrell, Jr, Gerhard Wagner & Volker Dötsch
Published online: 11 January 2007 | doi:10.1038/nprot.2006.181
Abstract - | Full Text - Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy | PDF (338 KB) - Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy
Preparation and square wave electroporation of retinal explant cultures - pp2710 - 2718
Stacy L Donovan & Michael A Dyer
Published online: 08 February 2007 | doi:10.1038/nprot.2006.454
Abstract - | Full Text - Preparation and square wave electroporation of retinal explant cultures | PDF (372 KB) - Preparation and square wave electroporation of retinal explant cultures | Supplementary information
Immunohistochemical staining of post-mortem adult human brain sections - pp2719 - 2732
Henry J Waldvogel, Maurice A Curtis, Kristin Baer, Mark I Rees & Richard L M Faull
Published online: 11 January 2006 | doi:10.1038/nprot.2006.354
Abstract - | Full Text - Immunohistochemical staining of post-mortem adult human brain sections | PDF (863 KB) - Immunohistochemical staining of post-mortem adult human brain sections
Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism - pp2733 - 2741
Norma J Greenfield
Published online: 11 January 2007 | doi:10.1038/nprot.2006.229
Abstract - | Full Text - Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism | PDF (272 KB) - Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism | Supplementary information
See also: Protocol by Greenfield | Protocol by Greenfield | Protocol by Greenfield
3' End cDNA amplification using classic RACE - pp2742 - 2745
Elizabeth Scotto–Lavino, Guangwei Du & Michael A Frohman
Published online: 11 January 2007 | doi:10.1038/nprot.2006.481
Abstract - | Full Text - 3' End cDNA amplification using classic RACE | PDF (158 KB) - 3' End cDNA amplification using classic RACE
See also: Protocol by Scotto-Lavino et al. | Protocol by Scotto–Lavino et al.
Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions - pp2746 - 2752
Naeem H Syed & Andrew J Flavell
Published online: 18 January 2007 | doi:10.1038/nprot.2006.407
Abstract - | Full Text - Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions | PDF (284 KB) - Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions
Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications - pp2753 - 2758
Navneeta Rajan, Jason Habermehl, Marie-France Coté, Charles J Doillon & Diego Mantovani
Published online: 18 January 2007 | doi:10.1038/nprot.2006.430
Abstract - | Full Text - Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications | PDF (213 KB) - Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications
Use of a commercially available reagent for the selective detection of homocysteine in plasma - pp2759 - 2762
Jorge O Escobedo, Weihua Wang & Robert M Strongin
Published online: 18 January 2007 | doi:10.1038/nprot.2006.399
Abstract - | Full Text - Use of a commercially available reagent for the selective detection of homocysteine in plasma | PDF (147 KB) - Use of a commercially available reagent for the selective detection of homocysteine in plasma
Long-term primary culture of secretory cells of Bothrops jararaca venom gland for venom production in vitro - pp2763 - 2766
Norma Yamanouye, Celine Marie Kerchove, Ana Maria Moura-da-Silva, Sylvia M Carneiro & Regina P Markus
Published online: 25 January 2007 | doi:10.1038/nprot.2006.423
Abstract - | Full Text - Long-term primary culture of secretory cells of Bothrops jararaca venom gland for venom production in vitro | PDF (1,482 KB) - Long-term primary culture of secretory cells of Bothrops jararaca venom gland for venom production in vitro
Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia - pp2767 - 2773
Martin Schaller, Katherina Zakikhany, Julian R Naglik, Günther Weindl & Bernhard Hube
Published online: 18 January 2007 | doi:10.1038/nprot.2006.474
Abstract - | Full Text - Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia | PDF (603 KB) - Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia
Restriction landmark genomic scanning - pp2774 - 2783
Yoshinari Ando & Yoshihide Hayashizaki
Published online: 25 January 2007 | doi:10.1038/nprot.2006.350
Abstract - | Full Text - Restriction landmark genomic scanning | PDF (3,576 KB) - Restriction landmark genomic scanning
Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS - pp2784 - 2790
Josip Blonder, King C Chan, Haleem J Issaq & Timothy D Veenstra
Published online: 18 January 2007 | doi:10.1038/nprot.2006.359
Abstract - | Full Text - Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS | PDF (283 KB) - Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS
Biochemical assays for multiple activation states of protein kinase C - pp2791 - 2795
Toshiyoshi Yamamoto, Hidenori Matsuzaki, Shinji Kamada, Yoshitaka Ono & Ushio Kikkawa
Published online: 25 January 2007 | doi:10.1038/nprot.2006.420
Abstract - | Full Text - Biochemical assays for multiple activation states of protein kinase C | PDF (1,981 KB) - Biochemical assays for multiple activation states of protein kinase C
A protocol for Agrobacterium-mediated transformation in rice - pp2796 - 2802
Asuka Nishimura, Ikuko Aichi & Makoto Matsuoka
Published online: 18 January 2007 | doi:10.1038/nprot.2006.469
Abstract - | Full Text - A protocol for Agrobacterium-mediated transformation in rice | PDF (296 KB) - A protocol for Agrobacterium-mediated transformation in rice
Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny - pp2803 - 2812
Jeffrey A Biernaskie, Ian A McKenzie, Jean G Toma & Freda D Miller
Published online: 25 January 2007 | doi:10.1038/nprot.2006.422
Abstract - | Full Text - Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny | PDF (2,470 KB) - Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny
Electron cryotomography sample preparation using the Vitrobot - pp2813 - 2819
Cristina V Iancu, William F Tivol, Jordan B Schooler, D Prabha Dias, Gregory P Henderson, Gavin E Murphy, Elizabeth R Wright, Zhuo Li, Zhiheng Yu, Ariane Briegel, Lu Gan, Yongning He & Grant J Jensen
Published online: 18 January 2006 | doi:10.1038/nprot.2006.432
Abstract - | Full Text - Electron cryotomography sample preparation using the Vitrobot | PDF (217 KB) - Electron cryotomography sample preparation using the Vitrobot
An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach - pp2820 - 2827
Arthur Tsai & Russ P Carstens
Published online: 18 January 2007 | doi:10.1038/nprot.2006.371
Abstract - | Full Text - An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach | PDF (252 KB) - An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach
Assessing hoarding in mice - pp2828 - 2830
Robert M J Deacon
Published online: 18 January 2007 | doi:10.1038/nprot.2006.171
Abstract - | Full Text - Assessing hoarding in mice | PDF (224 KB) - Assessing hoarding in mice
Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling - pp2831 - 2838
Jean-Philippe Pichon, Bertrand Bonnaud & François Mallet
Published online: 25 January 2007 | doi:10.1038/nprot.2006.475
Abstract - | Full Text - Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling | PDF (223 KB) - Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling
ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos - pp2839 - 2855
Thomas Sandmann, Janus S Jakobsen & Eileen E M Furlong
Published online: 25 January 2007 | doi:10.1038/nprot.2006.383
Abstract - | Full Text - ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos | PDF (595 KB) - ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos
In-gel digestion for mass spectrometric characterization of proteins and proteomes - pp2856 - 2860
Andrej Shevchenko,
Henrik Tomas,
Jan Havli
,
Jesper V Olsen
&
Matthias Mann
Published online: 25 January 2007 | doi:10.1038/nprot.2006.468
Abstract - | Full Text - In-gel digestion for mass spectrometric characterization of proteins and proteomes | PDF (291 KB) - In-gel digestion for mass spectrometric characterization of proteins and proteomes
See also: Protocol by Ong & Mann
Synthesis of a biocleavable polyrotaxane-plasmid DNA (pDNA) polyplex and its use for the rapid nonviral delivery of pDNA to cell nuclei - pp2861 - 2869
Atsushi Yamashita, Nobuhiko Yui, Tooru Ooya, Arihiro Kano, Atsushi Maruyama, Hidetaka Akita, Kentaro Kogure & Hideyoshi Harashima
Published online: 25 January 2007 | doi:10.1038/nprot.2006.438
Abstract - | Full Text - Synthesis of a biocleavable polyrotaxane-plasmid DNA (pDNA) polyplex and its use for the rapid nonviral delivery of pDNA to cell nuclei | PDF (437 KB) - Synthesis of a biocleavable polyrotaxane-plasmid DNA (pDNA) polyplex and its use for the rapid nonviral delivery of pDNA to cell nuclei
Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung - pp2870 - 2875
Kerstin A Sauer, Petra Scholtes, Roman Karwot & Susetta Finotto
Published online: 25 January 2007 | doi:10.1038/nprot.2006.435
Abstract - | Full Text - Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung | PDF (409 KB) - Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung
Using circular dichroism spectra to estimate protein secondary structure - pp2876 - 2890
Norma J Greenfield
Published online: 25 January 2007 | doi:10.1038/nprot.2006.202
Abstract - | Full Text - Using circular dichroism spectra to estimate protein secondary structure | PDF (398 KB) - Using circular dichroism spectra to estimate protein secondary structure | Supplementary information
Analysis of the kinetics of folding of proteins and peptides using circular dichroism - pp2891 - 2899
Norma J Greenfield
Published online: 25 January 2007 | doi:10.1038/nprot.2006.244
Abstract - | Full Text - Analysis of the kinetics of folding of proteins and peptides using circular dichroism | PDF (317 KB) - Analysis of the kinetics of folding of proteins and peptides using circular dichroism | Supplementary information
High resolution colonoscopy in live mice - pp2900 - 2904
C Becker, M C Fantini & M F Neurath
Published online: 25 January 2007 | doi:10.1038/nprot.2006.446
Abstract - | Full Text - High resolution colonoscopy in live mice | PDF (288 KB) - High resolution colonoscopy in live mice
Assays for in vitro monitoring of proliferation of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cells - pp2905 - 2908
Elena A Goncharova, Poay Lim, Dmitry A Goncharov, Andrew Eszterhas, Reynold A Panettieri, Jr & Vera P Krymskaya
Published online: 25 January 2007 | doi:10.1038/nprot.2006.433
Abstract - | Full Text - Assays for in vitro monitoring of proliferation of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cells | PDF (172 KB) - Assays for in vitro monitoring of proliferation of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cells
Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease - pp2909 - 2915
Linda I Nagore, Sucharita Mitra, Daifeng Jiang, Shoulei Jiang, YanWen Zhou, Magda Loranc & Harry W Jarrett
Published online: 25 January 2007 | doi:10.1038/nprot.2006.439
Abstract - | Full Text - Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease | PDF (256 KB) - Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease
Imaging synaptic vesicle exocytosis and endocytosis with FM dyes - pp2916 - 2921
Michael A Gaffield & William J Betz
Published online: 31 January 2007 | doi:10.1038/nprot.2006.476
Abstract - | Full Text - Imaging synaptic vesicle exocytosis and endocytosis with FM dyes | PDF (285 KB) - Imaging synaptic vesicle exocytosis and endocytosis with FM dyes | Supplementary information
A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 - pp2922 - 2932
Qian Li & Young-Tae Chang
Published online: 25 January 2007 | doi:10.1038/nprot.2006.484
Abstract - | Full Text - A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 | PDF (374 KB) - A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22
Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration - pp2933 - 2939
Elena A Goncharova, Dmitry A Goncharov & Vera P Krymskaya
Published online: 25 January 2007 | doi:10.1038/nprot.2006.434
Abstract - | Full Text - Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration | PDF (201 KB) - Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration
Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics - pp2940 - 2956
Tadashi Kondo & Setsuo Hirohashi
Published online: 25 January 2007 | doi:10.1038/nprot.2006.421
Abstract - | Full Text - Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics | PDF (2,786 KB) - Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics
Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code - pp2957 - 2962
Nobumasa Hino, Akiko Hayashi, Kensaku Sakamoto & Shigeyuki Yokoyama
Published online: 25 January 2007 | doi:10.1038/nprot.2006.424
Abstract - | Full Text - Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code | PDF (1,485 KB) - Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code
Fabrication of cells containing gel modules to assemble modular tissue-engineered constructs - pp2963 - 2969
Alison P McGuigan, Brendan Leung & Michael V Sefton
Published online: 25 January 2007 | doi:10.1038/nprot.2006.443
Abstract - | Full Text - Fabrication of cells containing gel modules to assemble modular tissue-engineered constructs | PDF (2,086 KB) - Fabrication of cells containing gel modules to assemble modular tissue-engineered constructs | Supplementary information
Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin - pp2970 - 2978
Juan Burrone, Zhiying Li & Venkatesh N Murthy
Published online: 25 January 2007 | doi:10.1038/nprot.2006.449
Abstract - | Full Text - Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin | PDF (434 KB) - Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin | Supplementary information
Adult mouse hematopoietic stem cells: purification and single-cell assays - pp2979 - 2987
Hideo Ema, Yohei Morita, Satoshi Yamazaki, Azusa Matsubara, Jun Seita, Yuko Tadokoro, Hiroyoshi Kondo, Hina Takano & Hiromitsu Nakauchi
Published online: 25 January 2007 | doi:10.1038/nprot.2006.447
Abstract - | Full Text - Adult mouse hematopoietic stem cells: purification and single-cell assays | PDF (415 KB) - Adult mouse hematopoietic stem cells: purification and single-cell assays
Whole-body imaging of bacterial infection and antibiotic response - pp2988 - 2994
Robert M Hoffman & Ming Zhao
Published online: 25 January 2007 | doi:10.1038/nprot.2006.376
Abstract - | Full Text - Whole-body imaging of bacterial infection and antibiotic response | PDF (10,500 KB) - Whole-body imaging of bacterial infection and antibiotic response
A simple method for quantitative determination of polysaccharides in fungal cell walls - pp2995 - 3000
Jean Marie François
Published online: 25 January 2007 | doi:10.1038/nprot.2006.457
Abstract - | Full Text - A simple method for quantitative determination of polysaccharides in fungal cell walls | PDF (203 KB) - A simple method for quantitative determination of polysaccharides in fungal cell walls
Dehydroalanine-containing peptides: preparation from phenylselenocysteine and utility in convergent ligation strategies - pp3001 - 3010
Matthew R Levengood & Wilfred A van der Donk
Published online: 25 January 2007 | doi:10.1038/nprot.2006.470
Abstract - | Full Text - Dehydroalanine-containing peptides: preparation from phenylselenocysteine and utility in convergent ligation strategies | PDF (343 KB) - Dehydroalanine-containing peptides: preparation from phenylselenocysteine and utility in convergent ligation strategies
Two-dimensional strandness-dependent electrophoresis - pp3011 - 3018
Gudmundur H Gunnarsson, Bjarki Gudmundsson, Hans G Thormar, Arni Alfredsson & Jon J Jonsson
Published online: 25 January 2007 | doi:10.1038/nprot.2006.477
Abstract - | Full Text - Two-dimensional strandness-dependent electrophoresis | PDF (292 KB) - Two-dimensional strandness-dependent electrophoresis
Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site–specific stable isotope tagging - pp3019 - 3027
Hiroyuki Kaji, Yoshio Yamauchi, Nobuhiro Takahashi & Toshiaki Isobe
Published online: 31 January 2007 | doi:10.1038/nprot.2006.444
Abstract - | Full Text - Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site–specific stable isotope tagging | PDF (1,435 KB) - Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site–specific stable isotope tagging
Contour-clamped homogeneous electric field electrophoresis of Staphylococcus aureus - pp3028 - 3033
Frances G O'Brien, Edet E Udo & Warren B Grubb
Published online: 25 January 2007 | doi:10.1038/nprot.2006.382
Abstract - | Full Text - Contour-clamped homogeneous electric field electrophoresis of Staphylococcus aureus | PDF (1,455 KB) - Contour-clamped homogeneous electric field electrophoresis of Staphylococcus aureus
In vitro assay for the quantitation of apoptosis-induced alterations of nuclear envelope permeability - pp3034 - 3040
Patricia Grote & Elisa Ferrando-May
Published online: 25 January 2007 | doi:10.1038/nprot.2006.460
Abstract - | Full Text - In vitro assay for the quantitation of apoptosis-induced alterations of nuclear envelope permeability | PDF (427 KB) - In vitro assay for the quantitation of apoptosis-induced alterations of nuclear envelope permeability
Synthesis of light-activated antisense oligodeoxynucleotide - pp3041 - 3048
XinJing Tang & Ivan J Dmochowski
Published online: 25 January 2007 | doi:10.1038/nprot.2006.462
Abstract - | Full Text - Synthesis of light-activated antisense oligodeoxynucleotide | PDF (279 KB) - Synthesis of light-activated antisense oligodeoxynucleotide
Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo - pp3049 - 3055
Ayumu Tashiro, Chunmei Zhao & Fred H Gage
Published online: 25 January 2007 | doi:10.1038/nprot.2006.473
Abstract - | Full Text - Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo | PDF (221 KB) - Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo
Amplification of 5' end cDNA with 'new RACE' - pp3056 - 3061
Elizabeth Scotto-Lavino, Guangwei Du & Michael A Frohman
Published online: 25 January 2007 | doi:10.1038/nprot.2006.479
Abstract - | Full Text - Amplification of 5' end cDNA with 'new RACE' | PDF (188 KB) - Amplification of 5' end cDNA with 'new RACE'
See also: Protocol by Scotto–Lavino et al. | Protocol by Scotto–Lavino et al.
Radiolabeling of TETA- and CB-TE2A-conjugated peptides with copper-64 - pp3062 - 3068
Thaddeus J Wadas & Carolyn J Anderson
Published online: 25 January 2007 | doi:10.1038/nprot.2006.431
Abstract - | Full Text - Radiolabeling of TETA- and CB-TE2A-conjugated peptides with copper-64 | PDF (198 KB) - Radiolabeling of TETA- and CB-TE2A-conjugated peptides with copper-64
PET imaging of herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk reporter gene expression in mice and humans using [18F]FHBG - pp3069 - 3074
Shahriar S Yaghoubi & Sanjiv S Gambhir
Published online: 15 February 2007 | doi:10.1038/nprot.2006.459
Abstract - | Full Text - PET imaging of herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk reporter gene expression in mice and humans using [18F]FHBG | PDF (352 KB) - PET imaging of herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk reporter gene expression in mice and humans using [18F]FHBG | Supplementary information
Thermal and high pressure intramolecular Diels–Alder reaction of vinylsulfonamides - pp3076 - 3087
Victor O Rogachev & Peter Metz
Published online: 25 January 2007 | doi:10.1038/nprot.2006.463
Abstract - | Full Text - Thermal and high pressure intramolecular Diels–Alder reaction of vinylsulfonamides | PDF (442 KB) - Thermal and high pressure intramolecular Diels–Alder reaction of vinylsulfonamides
A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis - pp3088 - 3093
Kaichun Zhu, Huali Jin, Zhonghuai He, Qinghong Zhu & Bin Wang
Published online: 31 January 2007 | doi:10.1038/nprot.2006.452
Abstract - | Full Text - A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis | PDF (207 KB) - A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis
Isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays - pp3094 - 3100
Kevin M Folta & Lon S Kaufman
Published online: 25 January 2007 | doi:10.1038/nprot.2006.471
Abstract - | Full Text - Isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays | PDF (223 KB) - Isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays
Mutation screening using fluorescence multiplex denaturing gradient gel electrophoresis (FMD): detecting mutations in the BRCA1 gene - pp3101 - 3110
Shiyu Zhang, Graciela Kuperstein & Steven A Narod
Published online: 31 January 2007 | doi:10.1038/nprot.2006.445
Abstract - | Full Text - Mutation screening using fluorescence multiplex denaturing gradient gel electrophoresis (FMD): detecting mutations in the BRCA1 gene | PDF (339 KB) - Mutation screening using fluorescence multiplex denaturing gradient gel electrophoresis (FMD): detecting mutations in the BRCA1 gene
See also: Protocol by Gasser et al. | Protocol by Chiou et al. | Protocol by Lavebratt & Sengul
Fusion PCR and gene targeting in Aspergillus nidulans - pp3111 - 3120
Edyta Szewczyk, Tania Nayak, C Elizabeth Oakley, Heather Edgerton, Yi Xiong, Naimeh Taheri-Talesh, Stephen A Osmani & Berl R Oakley
Published online: 31 January 2007 | doi:10.1038/nprot.2006.405
Abstract - | Full Text - Fusion PCR and gene targeting in Aspergillus nidulans | PDF (1,715 KB) - Fusion PCR and gene targeting in Aspergillus nidulans
Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation - pp3121 - 3128
Robin B Gasser, Min Hu, Neil B Chilton, Bronwyn E Campbell, Aaron J Jex, Domenico Otranto, Claudia Cafarchia, Ian Beveridge & Xingquan Zhu
Published online: 31 January 2007 | doi:10.1038/nprot.2006.485
Abstract - | Full Text - Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation | PDF (239 KB) - Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation
See also: Protocol by Zhang et al. | Protocol by Chiou et al. | Protocol by Lavebratt & Sengul
Spectral karyotyping analysis of human and mouse chromosomes - pp3129 - 3142
Hesed M Padilla-Nash, Linda Barenboim-Stapleton, Michael J Difilippantonio & Thomas Ried
Published online: 31 January 2007 | doi:10.1038/nprot.2006.358
Abstract - | Full Text - Spectral karyotyping analysis of human and mouse chromosomes | PDF (2,228 KB) - Spectral karyotyping analysis of human and mouse chromosomes
Programmable reactivity-based one-pot oligosaccharide synthesis - pp3143 - 3152
Jinq-Chyi Lee, William A Greenberg & Chi-Huey Wong
Published online: 31 January 2007 | doi:10.1038/nprot.2006.489
Abstract - | Full Text - Programmable reactivity-based one-pot oligosaccharide synthesis | PDF (267 KB) - Programmable reactivity-based one-pot oligosaccharide synthesis
Off-wafer fabrication and surface modification of asymmetric 3D SU-8 microparticles - pp3153 - 3158
Sarah L Tao, Ketul Popat & Tejal A Desai
Published online: 31 January 2007 | doi:10.1038/nprot.2006.451
Abstract - | Full Text - Off-wafer fabrication and surface modification of asymmetric 3D SU-8 microparticles | PDF (1,587 KB) - Off-wafer fabrication and surface modification of asymmetric 3D SU-8 microparticles
Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method - pp3159 - 3165
Irfan Rahman, Aruna Kode & Saibal K Biswas
Published online: 31 January 2007 | doi:10.1038/nprot.2006.378
Abstract - | Full Text - Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method | PDF (1,347 KB) - Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method
Stereotaxic gene delivery in the rodent brain - pp3166 - 3173
Ali Cetin, Shoji Komai, Marina Eliava, Peter H Seeburg & Pavel Osten
Published online: 31 January 2007 | doi:10.1038/nprot.2006.450
Abstract - | Full Text - Stereotaxic gene delivery in the rodent brain | PDF (1,569 KB) - Stereotaxic gene delivery in the rodent brain