An isothermal primer extension method for whole genome amplification of fresh and degraded DNA: applications in comparative genomic hybridization, genotyping and mutation screening - pp2185 - 2194

Cheryl I P Lee, Siew Hong Leong, Adrian E H Png, Keng Wah Choo, Christopher Syn, Dennis T H Lim, Hai Yang Law & Oi Lian Kon

Published online: 14 December 2006 | doi:10.1038/nprot.2006.398

Abstract - | Full Text - An isothermal primer extension method for whole genome amplification of fresh and degraded DNA: applications in comparative genomic hybridization, genotyping and mutation screening | PDF (1,042 KB) - An isothermal primer extension method for whole genome amplification of fresh and degraded DNA: applications in comparative genomic hybridization, genotyping and mutation screening

See also: Protocol by Till et al. | Protocol by Tabone et al. | Protocol by Singh & Thomas

Xenopus laevis transgenesis by sperm nuclear injection - pp2195 - 2203

Stuart J Smith, Lynne Fairclough, Branko V Latinkic, Duncan B Sparrow & Timothy J Mohun

Published online: 14 December 2006 | doi:10.1038/nprot.2006.325

Abstract - | Full Text - Xenopus laevis transgenesis by sperm nuclear injection | PDF (412 KB) - Xenopus laevis transgenesis by sperm nuclear injection | Supplementary information

Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels - pp2204 - 2212

Christina Lorenz, Frederike von Pelchrzim & Renée Schroeder

Published online: 14 December 2006 | doi:10.1038/nprot.2006.372

Abstract - | Full Text - Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels | PDF (281 KB) - Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels

Verification of automated peptide identifications from proteomic tandem mass spectra - pp2213 - 2222

David L Tabb, David B Friedman & Amy-Joan L Ham

Published online: 14 December 2006 | doi:10.1038/nprot.2006.330

Abstract - | Full Text - Verification of automated peptide identifications from proteomic tandem mass spectra | PDF (496 KB) - Verification of automated peptide identifications from proteomic tandem mass spectra

Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene - pp2223 - 2226

Andreas K Nussler, Matthias Glanemann, Anja Schirmeier, Liegang Liu & Natascha C Nüssler

Published online: 14 December 2006 | doi:10.1038/nprot.2006.341

Abstract - | Full Text - Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene | PDF (138 KB) - Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene

A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii - pp2227 - 2230

Catherine B Mason, Terry M Bricker & James V Moroney

Published online: 14 December 2006 | doi:10.1038/nprot.2006.348

Abstract - | Full Text - A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii | PDF (273 KB) - A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii

Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized libraries - pp2231 - 2240

Giovanni M Lesa

Published online: 14 December 2006 | doi:10.1038/nprot.2006.345

Abstract - | Full Text - Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized libraries | PDF (2,035 KB) - Isolation of Caenorhabditis elegans gene knockouts by PCR screening of chemically mutagenized libraries | Supplementary information

PEGylation of native disulfide bonds in proteins - pp2241 - 2252

Steve Brocchini, Sibu Balan, Antony Godwin, Ji-Won Choi, Mire Zloh & Sunil Shaunak

Published online: 14 December 2006 | doi:10.1038/nprot.2006.346

Abstract - | Full Text - PEGylation of native disulfide bonds in proteins | PDF (395 KB) - PEGylation of native disulfide bonds in proteins

Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red - pp2253 - 2256

Arthur F J Ram & Frans M Klis

Published online: 14 December 2006 | doi:10.1038/nprot.2006.397

Abstract - | Full Text - Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red | PDF (191 KB) - Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red

Recombinant protein purification by self-cleaving aggregation tag - pp2257 - 2262

Wan-Yi Wu, Courtney Mee, Filomena Califano, Reza Banki & David W Wood

Published online: 14 December 2006 | doi:10.1038/nprot.2006.314

Abstract - | Full Text - Recombinant protein purification by self-cleaving aggregation tag | PDF (218 KB) - Recombinant protein purification by self-cleaving aggregation tag

See also: Protocol by Arnau et al.

Collection of lymph-borne dendritic cells in the rat - pp2263 - 2270

Simon WF Milling, Christopher Jenkins & Gordon MacPherson

Published online: 21 December 2006 | doi:10.1038/nprot.2006.315

Abstract - | Full Text - Collection of lymph-borne dendritic cells in the rat | PDF (512 KB) - Collection of lymph-borne dendritic cells in the rat

Estimation of baculovirus titer based on viable cell size - pp2271 - 2276

Vasantharajan Janakiraman, William F Forrest & Somasekar Seshagiri

Published online: 21 December 2006 | doi:10.1038/nprot.2006.387

Abstract - | Full Text - Estimation of baculovirus titer based on viable cell size | PDF (190 KB) - Estimation of baculovirus titer based on viable cell size

Administration and interpretation of the Trail Making Test - pp2277 - 2281

Christopher R Bowie & Philip D Harvey

Published online: 21 December 2006 | doi:10.1038/nprot.2006.390

Abstract - | Full Text - Administration and interpretation of the Trail Making Test | PDF (134 KB) - Administration and interpretation of the Trail Making Test

Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassays - pp2282 - 2287

Yael Weiss, Baruch Rubin, Avidor Shulman, Irina Ben Shir, Ehud Keinan & Shmuel Wolf

Published online: 21 December 2006 | doi:10.1038/nprot.2006.380

Abstract - | Full Text - Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassays | PDF (335 KB) - Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassays

Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic–endothelial sinusoid-like structures - pp2288 - 2296

Yaakov Nahmias & David J Odde

Published online: 21 December 2006 | doi:10.1038/nprot.2006.386

Abstract - | Full Text - Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic–endothelial sinusoid-like structures | PDF (371 KB) - Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic–endothelial sinusoid-like structures

Chemical cleavage of mismatch (CCM) to locate base mismatches in heteroduplex DNA - pp2297 - 2304

Tania Tabone, Georgina Sallmann, Maria Chiotis, Matthew Law & Richard Cotton

Published online: 21 December 2006 | doi:10.1038/nprot.2006.352

Abstract - | Full Text - Chemical cleavage of mismatch (CCM) to locate base mismatches in heteroduplex DNA | PDF (363 KB) - Chemical cleavage of mismatch (CCM) to locate base mismatches in heteroduplex DNA

See also: Protocol by Till et al. | Protocol by Singh & Thomas | Protocol by Lee et al.

Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts - pp2305 - 2314

Eva Hannak & Rebecca Heald

Published online: 21 December 2006 | doi:10.1038/nprot.2006.396

Abstract - | Full Text - Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts | PDF (291 KB) - Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts

Clonogenic assay of cells in vitro - pp2315 - 2319

Nicolaas A P Franken, Hans M Rodermond, Jan Stap, Jaap Haveman & Chris van Bree

Published online: 21 December 2006 | doi:10.1038/nprot.2006.339

Abstract - | Full Text - Clonogenic assay of cells in vitro | PDF (195 KB) - Clonogenic assay of cells in vitro

A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide - pp2320 - 2325

G C Allen, M A Flores-Vergara, S Krasynanski, S Kumar & W F Thompson

Published online: 21 December 2006 | doi:10.1038/nprot.2006.384

Abstract - | Full Text - A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide | PDF (195 KB) - A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags - pp2326 - 2333

José Arnau, Conni Lauritzen & John Pedersen

Published online: 21 December 2006 | doi:10.1038/nprot.2006.388

Abstract - | Full Text - Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags | PDF (243 KB) - Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags

See also: Protocol by Wu et al.

Cell culture and infection system for hepatitis C virus - pp2334 - 2339

Takanobu Kato, Tomoko Date, Asako Murayama, Kenichi Morikawa, Daisuke Akazawa & Takaji Wakita

Published online: 21 December 2006 | doi:10.1038/nprot.2006.395

Abstract - | Full Text - Cell culture and infection system for hepatitis C virus | PDF (202 KB) - Cell culture and infection system for hepatitis C virus

High-throughput screening of activity and enantioselectivity of esterases - pp2340 - 2343

Dominique Böttcher & Uwe T Bornscheuer

Published online: 21 December 2006 | doi:10.1038/nprot.2006.391

Abstract - | Full Text - High-throughput screening of activity and enantioselectivity of esterases | PDF (204 KB) - High-throughput screening of activity and enantioselectivity of esterases

A method for the covalent capture and screening of diverse small molecules in a microarray format - pp2344 - 2352

James E Bradner, Olivia M McPherson & Angela N Koehler

Published online: 21 December 2006 | doi:10.1038/nprot.2006.282

Abstract - | Full Text - A method for the covalent capture and screening of diverse small molecules in a microarray format | PDF (315 KB) - A method for the covalent capture and screening of diverse small molecules in a microarray format | Supplementary information

DNA methylation: Bisulphite modification and analysis - pp2353 - 2364

Susan J Clark, Aaron Statham, Clare Stirzaker, Peter L Molloy & Marianne Frommer

Published online: 21 December 2006 | doi:10.1038/nprot.2006.324

Abstract - | Full Text - DNA methylation: Bisulphite modification and analysis | PDF (557 KB) - DNA methylation: Bisulphite modification and analysis

Flow cytometry and FISH to measure the average length of telomeres (flow FISH) - pp2365 - 2376

Gabriela M Baerlocher, Irma Vulto, Gary de Jong & Peter M Lansdorp

Published online: 21 December 2006 | doi:10.1038/nprot.2006.263

Abstract - | Full Text - Flow cytometry and FISH to measure the average length of telomeres (flow FISH) | PDF (368 KB) - Flow cytometry and FISH to measure the average length of telomeres (flow FISH) | Supplementary information

Synthesis of non-natural ManNAc analogs for the expression of thiols on cell-surface sialic acids - pp2377 - 2385

Srinivasa-Gopalan Sampathkumar, Adrienne V Li & Kevin J Yarema

Published online: 21 December 2006 | doi:10.1038/nprot.2006.319

Abstract - | Full Text - Synthesis of non-natural ManNAc analogs for the expression of thiols on cell-surface sialic acids | PDF (287 KB) - Synthesis of non-natural ManNAc analogs for the expression of thiols on cell-surface sialic acids

Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays - pp2386 - 2398

Souvik Chattopadhaya, Lay Pheng Tan & Shao Q Yao

Published online: 21 December 2006 | doi:10.1038/nprot.2006.338

Abstract - | Full Text - Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays | PDF (481 KB) - Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays

Time-lapse imaging of dendritic spines in vitro - pp2399 - 2405

J Martin Verkuyl & Andrew Matus

Published online: 21 December 2006 | doi:10.1038/nprot.2006.357

Abstract - | Full Text - Time-lapse imaging of dendritic spines in vitro | PDF (306 KB) - Time-lapse imaging of dendritic spines in vitro | Supplementary information

Culturing hippocampal neurons - pp2406 - 2415

Stefanie Kaech & Gary Banker

Published online: 11 January 2007 | doi:10.1038/nprot.2006.356

Abstract - | Full Text - Culturing hippocampal neurons | PDF (1,727 KB) - Culturing hippocampal neurons

See also: Protocol by Gogolla et al.

Extraction and analysis of soluble inositol polyphosphates from yeast - pp2416 - 2422

Cristina Azevedo & Adolfo Saiardi

Published online: 21 December 2006 | doi:10.1038/nprot.2006.337

Abstract - | Full Text - Extraction and analysis of soluble inositol polyphosphates from yeast | PDF (365 KB) - Extraction and analysis of soluble inositol polyphosphates from yeast

See also: Protocol by Gregan et al.

A fluorescence-based protocol for quantifying angiotensin-converting enzyme activity - pp2423 - 2427

Miguel Ángel Sentandreu & Fidel Toldrá

Published online: 29 December 2006 | doi:10.1038/nprot.2006.349

Abstract - | Full Text - A fluorescence-based protocol for quantifying angiotensin-converting enzyme activity | PDF (151 KB) - A fluorescence-based protocol for quantifying angiotensin-converting enzyme activity

Multiplex-terminal restriction fragment length polymorphism - pp2428 - 2433

Brajesh K Singh & Nadine Thomas

Published online: 29 December 2006 | doi:10.1038/nprot.2006.392

Abstract - | Full Text - Multiplex-terminal restriction fragment length polymorphism | PDF (232 KB) - Multiplex-terminal restriction fragment length polymorphism | Supplementary information

See also: Protocol by Till et al. | Protocol by Tabone et al. | Protocol by Lee et al.

Triflimide (HNTf₂)–catalyzed aldehyde cross-aldol reaction using "super silyl" enol ethers - pp2434 - 2438

Matthew B Boxer & Hisashi Yamamoto

Published online: 29 December 2006 | doi:10.1038/nprot.2006.389

Abstract - | Full Text - Triflimide (HNTf2)–catalyzed aldehyde cross-aldol reaction using "super silyl" enol ethers | PDF (210 KB) - Triflimide (HNTf2)–catalyzed aldehyde cross-aldol reaction using "super silyl" enol ethers

Identification of eukaryotic secreted and cell surface proteins using the yeast secretion trap screen - pp2439 - 2447

Sang-Jik Lee, Byung-Dong Kim & Jocelyn K C Rose

Published online: 29 December 2006 | doi:10.1038/nprot.2006.373

Abstract - | Full Text - Identification of eukaryotic secreted and cell surface proteins using the yeast secretion trap screen | PDF (290 KB) - Identification of eukaryotic secreted and cell surface proteins using the yeast secretion trap screen

Measuring the optokinetic response of zebrafish larvae - pp2448 - 2451

Susan E Brockerhoff

Published online: 29 December 2006 | doi:10.1038/nprot.2006.255

Abstract - | Full Text - Measuring the optokinetic response of zebrafish larvae | PDF (176 KB) - Measuring the optokinetic response of zebrafish larvae | Supplementary information

Staining protocol for organotypic hippocampal slice cultures - pp2452 - 2456

Nadine Gogolla, Ivan Galimberti, Vincenzo DePaola & Pico Caroni

Published online: 29 December 2006 | doi:10.1038/nprot.2006.180

Abstract - | Full Text - Staining protocol for organotypic hippocampal slice cultures | PDF (279 KB) - Staining protocol for organotypic hippocampal slice cultures

See also: Protocol by Kaech & Banker

High-throughput knockout screen in fission yeast - pp2457 - 2464

Juraj Gregan, Peter K Rabitsch, Cornelia Rumpf, Maria Novatchkova, Alexander Schleiffer & Kim Nasmyth

Published online: 29 December 2006 | doi:10.1038/nprot.2006.385

Abstract - | Full Text - High-throughput knockout screen in fission yeast | PDF (737 KB) - High-throughput knockout screen in fission yeast

See also: Protocol by Azevedo & Saiardi

A protocol for TILLING and Ecotilling in plants and animals - pp2465 - 2477

Bradley J Till, Troy Zerr, Luca Comai & Steven Henikoff

Published online: 29 December 2006 | doi:10.1038/nprot.2006.329

Abstract - | Full Text - A protocol for TILLING and Ecotilling in plants and animals | PDF (484 KB) - A protocol for TILLING and Ecotilling in plants and animals

See also: Protocol by Singh & Thomas | Protocol by Tabone et al. | Protocol by Lee et al.

IRAP and REMAP for retrotransposon-based genotyping and fingerprinting - pp2478 - 2484

Ruslan Kalendar & Alan H Schulman

Published online: 11 January 2007 | doi:10.1038/nprot.2006.377

Abstract - | Full Text - IRAP and REMAP for retrotransposon-based genotyping and fingerprinting | PDF (305 KB) - IRAP and REMAP for retrotransposon-based genotyping and fingerprinting

One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities - pp2485 - 2492

Hai Yu, Harshal A. Chokhawala, Shengshu Huang & Xi Chen

Published online: 11 January 2007 | doi:10.1038/nprot.2006.401

Abstract - | Full Text - One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities | PDF (1,483 KB) - One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities

Error-prone rolling circle amplification: the simplest random mutagenesis protocol - pp2493 - 2497

Ryota Fujii, Motomitsu Kitaoka & Kiyoshi Hayashi

Published online: 29 December 2006 | doi:10.1038/nprot.2006.403

Abstract - | Full Text - Error-prone rolling circle amplification: the simplest random mutagenesis protocol | PDF (277 KB) - Error-prone rolling circle amplification: the simplest random mutagenesis protocol

A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes - pp2498 - 2505

Andrew Holt & Monica M Palcic

Published online: 29 December 2006 | doi:10.1038/nprot.2006.402

Abstract - | Full Text - A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes | PDF (245 KB) - A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes

Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants - pp2506 - 2511

Christopher R Thornton & Nicholas J Talbot

Published online: 29 December 2006 | doi:10.1038/nprot.2006.347

Abstract - | Full Text - Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants | PDF (302 KB) - Immunofluorescence microscopy and immunogold EM for investigating fungal infection of plants

Collagen antibody-induced arthritis - pp2512 - 2516

Levon M Khachigian

Published online: 29 December 2006 | doi:10.1038/nprot.2006.393

Abstract - | Full Text - Collagen antibody-induced arthritis | PDF (285 KB) - Collagen antibody-induced arthritis

Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique - pp2517 - 2526

Aysha H Osmani, Berl R Oakley & Stephen A Osmani

Published online: 29 December 2006 | doi:10.1038/nprot.2006.406

Abstract - | Full Text - Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique | PDF (387 KB) - Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique