Protocol abstract
Nature Protocols 1, - 2320 - 2325 (2006)
Published online: 21 December 2006 | Corrected online: 1 July 0703 | doi:10.1038/nprot.2006.384
Subject Categories: Isolation, purification and separation | Model organisms
A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide
G C Allen1,5, M A Flores-Vergara2,5, S Krasynanski3,5, S Kumar2,5 & W F Thompson4,5
Abstract
We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5–30 
g of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5–6 h.
Note: In the version of this article initially published online, the name of the coauthor S Krasnyanski was misspelled as S Krasynanski. This error has been corrected in all versions of the article.
- Departments of Horticultural Science and Crop Science, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, NC 27606-7550, USA.
- Department of Plant Biology, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, NC 27606-7550, USA.
- Department of Crop Science, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, NC 27606-7550, USA.
- Departments of Plant Biology, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, NC 27606-7550, USA.
- All authors contributed equally to this work.
Correspondence to: G C Allen1,5 e-mail: george_allen@ncsu.edu
