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Technique for infiltrating Agrobacterium into tobacco epidermal cells for transient expression of fluorescent fusion proteins. The top of the leaf is supported with a finger while a syringe containing Agrobacterium in infiltration medium is placed over a small needle mark on the lower epidermis. With gentle pressure on the plunger, liquid fills the mesophylar air spaces of the cell. From the protocol by Sparkes et al. Cover by Jessica Iannuzzi.