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Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images

Alfredo Rodriguez, Douglas B Ehlenberger, Patrick R Hof & Susan L Wearne

Nature Protocols 1, 2152 - 2161 (2006) Published online: 7 December 2006

doi:10.1038/nprot.2006.313

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Figure 1 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

Figure 1

Recursive subdivision of a regular octahedron to obtain a geodesic unit sphere from which the Rayburst sampling core, with an associated triangular surface mesh, can be derived.

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Figure 2

Iterative computation of face of exit of a ray.

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Figure 3

Dendritic branch diameter estimation by 2D Rayburst, irrespective of orientation of branch within image stack.

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Figure 4

Estimation of volume and surface area of a dendritic spine using Rayburst.

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Figure 5

Application of Rayburst to reconstruction of a spiny dendritic branch from a layer III pyramidal neuron from prefrontal area 46 in a rhesus monkey (a,b), and a thioflavine S-labeled amyloid plaque from a Tg2576 mouse (c,d).

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Table 1

Troubleshooting table.

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