Measuring calcium signaling using genetically targetable fluorescent indicators - pp1057 - 1065

Amy E Palmer & Roger Y Tsien

Published online: 17 August 2006 | doi:10.1038/nprot.2006.172

Abstract - | Full Text - Measuring calcium signaling using genetically targetable fluorescent indicators | PDF (298 KB) - Measuring calcium signaling using genetically targetable fluorescent indicators

Selection and characterization of large collections of peptide aptamers through optimized yeast two-hybrid procedures - pp1066 - 1091

Marc B T Bickle, Eric Dusserre, Olivier Moncorgé, Hélène Bottin & Pierre Colas

Published online: 17 August 2006 | doi:10.1038/nprot.2006.32

Abstract - | Full Text - Selection and characterization of large collections of peptide aptamers through optimized yeast two-hybrid procedures | PDF (659 KB) - Selection and characterization of large collections of peptide aptamers through optimized yeast two-hybrid procedures

Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation - pp1092 - 1104

Anna Tourovskaia, Xavier Figueroa-Masot & Albert Folch

Published online: 17 August 2006 | doi:10.1038/nprot.2006.123

Abstract - | Full Text - Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation | PDF (951 KB) - Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation

Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens - pp1105 - 1111

Kristin J Mayo, Barbara J Gonzales & Hugh S Mason

Published online: 17 August 2006 | doi:10.1038/nprot.2006.176

Abstract - | Full Text - Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens | PDF (181 KB) - Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens

Sulforhodamine B colorimetric assay for cytotoxicity screening - pp1112 - 1116

Vanicha Vichai & Kanyawim Kirtikara

Published online: 17 August 2006 | doi:10.1038/nprot.2006.179

Abstract - | Full Text - Sulforhodamine B colorimetric assay for cytotoxicity screening | PDF (141 KB) - Sulforhodamine B colorimetric assay for cytotoxicity screening

Assessing nest building in mice - pp1117 - 1119

Robert MJ Deacon

Published online: 24 August 2006 | doi:10.1038/nprot.2006.170

Abstract - | Full Text - Assessing nest building in mice | PDF (221 KB) - Assessing nest building in mice

Generation of peptide–MHC class I complexes through UV-mediated ligand exchange - pp1120 - 1132

Boris Rodenko, Mireille Toebes, Sine Reker Hadrup, Wim J E van Esch, Annemieke M Molenaar, Ton N M Schumacher & Huib Ovaa

Published online: 31 August 2006 | doi:10.1038/nprot.2006.121

Abstract - | Full Text - Generation of peptide–MHC class I complexes through UV-mediated ligand exchange | PDF (929 KB) - Generation of peptide–MHC class I complexes through UV-mediated ligand exchange | Supplementary information

Transgenesis in fish: efficient selection of transgenic fish by co-injection with a fluorescent reporter construct - pp1133 - 1139

Martina Rembold, Kajori Lahiri, Nicholas S. Foulkes & Joachim Wittbrodt

Published online: 07 September 2006 | doi:10.1038/nprot.2006.165

Abstract - | Full Text - Transgenesis in fish: efficient selection of transgenic fish by co-injection with a fluorescent reporter construct | PDF (520 KB) - Transgenesis in fish: efficient selection of transgenic fish by co-injection with a fluorescent reporter construct | Supplementary information

Stromal cell–dependent growth of B-1 B cell progenitors in the absence of direct contact - pp1140 - 1144

Encarnacion Montecino-Rodriguez & Kenneth Dorshkind

Published online: 07 September 2006 | doi:10.1038/nprot.2006.163

Abstract - | Full Text - Stromal cell–dependent growth of B-1 B cell progenitors in the absence of direct contact | PDF (181 KB) - Stromal cell–dependent growth of B-1 B cell progenitors in the absence of direct contact

Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos - pp1145 - 1153

Guy S Eakin & Anna-Katerina Hadjantonakis

Published online: 07 September 2006 | doi:10.1038/nprot.2006.173

Abstract - | Full Text - Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos | PDF (2,000 KB) - Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos | Supplementary information

220-plex microRNA expression profile of a single cell - pp1154 - 1159

Fuchou Tang, Petra Hajkova, Sheila C Barton, Dónal O'Carroll, Caroline Lee, Kaiqin Lao & M Azim Surani

Published online: 07 September 2006 | doi:10.1038/nprot.2006.161

Abstract - | Full Text - 220-plex microRNA expression profile of a single cell | PDF (202 KB) - 220-plex microRNA expression profile of a single cell

Creating self-illuminating quantum dot conjugates - pp1160 - 1164

Min-Kyung So, Andreas M Loening, Sanjiv S Gambhir & Jianghong Rao

Published online: 07 September 2006 | doi:10.1038/nprot.2006.162

Abstract - | Full Text - Creating self-illuminating quantum dot conjugates | PDF (248 KB) - Creating self-illuminating quantum dot conjugates

Preparation of organotypic hippocampal slice cultures for long-term live imaging - pp1165 - 1171

Nadine Gogolla, Ivan Galimberti, Vincenzo DePaola & Pico Caroni

Published online: 07 September 2006 | doi:10.1038/nprot.2006.168

Abstract - | Full Text - Preparation of organotypic hippocampal slice cultures for long-term live imaging | PDF (292 KB) - Preparation of organotypic hippocampal slice cultures for long-term live imaging

See also: Protocol by Davie et al. | Protocol by Gogolla et al. | Protocol by De Simoni & MY Yu | Protocol by O'Brien & Lummis

Multiplex-fluorescence in situ hybridization for chromosome karyotyping - pp1172 - 1184

Jochen B Geigl, Sabine Uhrig & Michael R Speicher

Published online: 07 September 2006 | doi:10.1038/nprot.2006.160

Abstract - | Full Text - Multiplex-fluorescence in situ hybridization for chromosome karyotyping | PDF (300 KB) - Multiplex-fluorescence in situ hybridization for chromosome karyotyping

Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis - pp1185 - 1193

Rinako Nakagawa, Susan M Mason & Alison M Michie

Published online: 07 September 2006 | doi:10.1038/nprot.2006.178

Abstract - | Full Text - Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis | PDF (493 KB) - Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis

Using drug-discrimination techniques to study the abuse-related effects of psychoactive drugs in rats - pp1194 - 1206

Marcello Solinas, Leigh V Panlilio, Zuzana Justinova, Sevil Yasar & Steven R Goldberg

Published online: 14 September 2006 | doi:10.1038/nprot.2006.167

Abstract - | Full Text - Using drug-discrimination techniques to study the abuse-related effects of psychoactive drugs in rats | PDF (288 KB) - Using drug-discrimination techniques to study the abuse-related effects of psychoactive drugs in rats

A radioactive uptake assay to measure ion transport across ion channel–containing liposomes - pp1207 - 1212

Crina M Nimigean

Published online: 14 September 2006 | doi:10.1038/nprot.2006.166

Abstract - | Full Text - A radioactive uptake assay to measure ion transport across ion channel–containing liposomes | PDF (631 KB) - A radioactive uptake assay to measure ion transport across ion channel–containing liposomes

Expanded-bed adsorption immobilized-metal affinity chromatography - pp1213 - 1222

Berend Tolner, Lisa Smith, Richard H J Begent & Kerry A Chester

Published online: 21 September 2006 | doi:10.1038/nprot.2006.127

Abstract - | Full Text - Expanded-bed adsorption immobilized-metal affinity chromatography | PDF (273 KB) - Expanded-bed adsorption immobilized-metal affinity chromatography | Supplementary information

Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures - pp1223 - 1226

Nadine Gogolla, Ivan Galimberti, Vincenzo DePaola & Pico Caroni

Published online: 21 September 2006 | doi:10.1038/nprot.2006.169

Abstract - | Full Text - Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures | PDF (187 KB) - Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures

See also: Protocol by Davie et al. | Protocol by Gogolla et al. | Protocol by De Simoni & MY Yu | Protocol by O'Brien & Lummis

Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome - pp1227 - 1234

Yoshihiro Sasaki, Kazuki Matsui, Yasuhiro Aoyama & Jun-ichi Kikuchi

Published online: 21 September 2006 | doi:10.1038/nprot.2006.182

Abstract - | Full Text - Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome | PDF (342 KB) - Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome

Dendritic patch-clamp recording - pp1235 - 1247

Jenny T Davie, Maarten H P Kole, Johannes J Letzkus, Ede A Rancz, Nelson Spruston, Greg J Stuart & Michael Häusser

Published online: 21 September 2006 | doi:10.1038/nprot.2006.164

Abstract - | Full Text - Dendritic patch-clamp recording | PDF (482 KB) - Dendritic patch-clamp recording | Supplementary information

See also: Protocol by Gogolla et al. | Protocol by De Simoni & MY Yu | Protocol by O'Brien & Lummis

Using phiC31 integrase to make transgenic Xenopus laevis embryos - pp1248 - 1257

Bryan G Allen & Daniel L Weeks

Published online: 21 September 2006 | doi:10.1038/nprot.2006.183

Abstract - | Full Text - Using phiC31 integrase to make transgenic Xenopus laevis embryos | PDF (325 KB) - Using phiC31 integrase to make transgenic Xenopus laevis embryos

Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins - pp1258 - 1266

Aaron R. Clapp, Ellen R. Goldman & Hedi Mattoussi

Published online: 28 September 2006 | doi:10.1038/nprot.2006.184

Abstract - | Full Text - Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins | PDF (274 KB) - Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins

In vivo single-cell electroporation for transfer of DNA and macromolecules - pp1267 - 1272

Jennifer E Bestman, Rebecca C Ewald, Shu-Ling Chiu & Hollis T Cline

Published online: 28 September 2006 | doi:10.1038/nprot.2006.186

Abstract - | Full Text - In vivo single-cell electroporation for transfer of DNA and macromolecules | PDF (385 KB) - In vivo single-cell electroporation for transfer of DNA and macromolecules

Assaying RNA chaperone activity in vivo in bacteria using a ribozyme folding trap - pp1273 - 1277

Silvia Prenninger, Renée Schroeder & Katharina Semrad

Published online: 05 October 2006 | doi:10.1038/nprot.2006.189

Abstract - | Full Text - Assaying RNA chaperone activity in vivo in bacteria using a ribozyme folding trap | PDF (246 KB) - Assaying RNA chaperone activity in vivo in bacteria using a ribozyme folding trap

Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells - pp1278 - 1286

Tom K Kerppola

Published online: 05 October 2006 | doi:10.1038/nprot.2006.201

Abstract - | Full Text - Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells | PDF (270 KB) - Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells

Immunocytochemistry and quantification of protein colocalization in cultured neurons - pp1287 - 1296

Marian W Glynn & A Kimberley McAllister

Published online: 05 October 2006 | doi:10.1038/nprot.2006.220

Abstract - | Full Text - Immunocytochemistry and quantification of protein colocalization in cultured neurons | PDF (560 KB) - Immunocytochemistry and quantification of protein colocalization in cultured neurons

Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish - pp1297 - 1305

Shannon Fisher, Elizabeth A Grice, Ryan M Vinton, Seneca L Bessling, Akihiro Urasaki, Koichi Kawakami & Andrew S McCallion

Published online: 12 October 2006 | doi:10.1038/nprot.2006.230

Abstract - | Full Text - Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish | PDF (349 KB) - Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish

Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory' - pp1306 - 1311

Rick A Bevins & Joyce Besheer

Published online: 12 October 2006 | doi:10.1038/nprot.2006.205

Abstract - | Full Text - Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory' | PDF (177 KB) - Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study 'recognition memory' | Supplementary information

High efficiency germ-line transformation of mosquitoes - pp1312 - 1317

Neil F Lobo, John R Clayton, Malcolm J Fraser, Fotis C Kafatos & Frank H Collins

Published online: 19 October 2006 | doi:10.1038/nprot.2006.221

Abstract - | Full Text - High efficiency germ-line transformation of mosquitoes | PDF (272 KB) - High efficiency germ-line transformation of mosquitoes

A general user interface for prediction servers of proteins' post-translational modification sites - pp1318 - 1321

Fengfeng Zhou, Yu Xue, Xuebiao Yao & Ying Xu

Published online: 26 October 2006 | doi:10.1038/nprot.2006.209

Abstract - | Full Text - A general user interface for prediction servers of proteins' post-translational modification sites | PDF (227 KB) - A general user interface for prediction servers of proteins' post-translational modification sites

Microcontact printing of axon guidance molecules for generation of graded patterns - pp1322 - 1328

Anne C von Philipsborn, Susanne Lang, André Bernard, Jürgen Loeschinger, Christian David, Dirk Lehnert, Martin Bastmeyer & Friedrich Bonhoeffer

Published online: 26 October 2006 | doi:10.1038/nprot.2006.251

Abstract - | Full Text - Microcontact printing of axon guidance molecules for generation of graded patterns | PDF (317 KB) - Microcontact printing of axon guidance molecules for generation of graded patterns

Design, construction and in vitro testing of zinc finger nucleases - pp1329 - 1341

Dana Carroll, J Jason Morton, Kelly J Beumer & David J Segal

Published online: 26 October 2006 | doi:10.1038/nprot.2006.231

Abstract - | Full Text - Design, construction and in vitro testing of zinc finger nucleases | PDF (508 KB) - Design, construction and in vitro testing of zinc finger nucleases | Supplementary information

Quantification of starch in plant tissues - pp1342 - 1345

Alison M Smith & Samuel C Zeeman

Published online: 26 October 2006 | doi:10.1038/nprot.2006.232

Abstract - | Full Text - Quantification of starch in plant tissues | PDF (123 KB) - Quantification of starch in plant tissues

Evolving proteins in mammalian cells using somatic hypermutation - pp1346 - 1350

Lei Wang & Roger Y Tsien

Published online: 26 October 2006 | doi:10.1038/nprot.2006.243

Abstract - | Full Text - Evolving proteins in mammalian cells using somatic hypermutation | PDF (164 KB) - Evolving proteins in mammalian cells using somatic hypermutation

Two-dimensional difference gel electrophoresis - pp1351 - 1358

Surya Viswanathan, Mustafa Ünlü & Jonathan S Minden

Published online: 26 October 2006 | doi:10.1038/nprot.2006.234

Abstract - | Full Text - Two-dimensional difference gel electrophoresis | PDF (293 KB) - Two-dimensional difference gel electrophoresis | Supplementary information

Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides - pp1359 - 1369

Maxim V Berezovski, Michael U Musheev, Andrei P Drabovich, Julia V Jitkova & Sergey N Krylov

Published online: 2 November 2006 | doi:10.1038/nprot.2006.200

Abstract - | Full Text - Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides | PDF (289 KB) - Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides

Developing multiplexed SNP assays with special reference to degraded DNA templates - pp1370 - 1378

Juan J Sanchez & Phillip Endicott

Published online: 02 November 2006 | doi:10.1038/nprot.2006.247

Abstract - | Full Text - Developing multiplexed SNP assays with special reference to degraded DNA templates | PDF (308 KB) - Developing multiplexed SNP assays with special reference to degraded DNA templates

Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium - pp1379 - 1391

Patrizia Camelliti, John O Gallagher, Peter Kohl & Andrew D McCulloch

Published online: 02 November 2006 | doi:10.1038/nprot.2006.203

Abstract - | Full Text - Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium | PDF (470 KB) - Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium

Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells - pp1392 - 1398

Musa M Mhlanga & Sanjay Tyagi

Published online: 02 November 2006 | doi:10.1038/nprot.2006.242

Abstract - | Full Text - Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells | PDF (182 KB) - Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells

BrdU assay for neurogenesis in rodents - pp1399 - 1405

J Martin Wojtowicz & Nohjin Kee

Published online: 02 November 2006 | doi:10.1038/nprot.2006.224

Abstract - | Full Text - BrdU assay for neurogenesis in rodents | PDF (269 KB) - BrdU assay for neurogenesis in rodents

Generation of transgenic birds with replication-deficient lentiviruses - pp1406 - 1411

Benjamin B Scott & Carlos Lois

Published online: 02 November 2006 | doi:10.1038/nprot.2006.187

Abstract - | Full Text - Generation of transgenic birds with replication-deficient lentiviruses | PDF (206 KB) - Generation of transgenic birds with replication-deficient lentiviruses

Production and characterization of adeno-associated viral vectors - pp1412 - 1428

Joshua C Grieger, Vivian W Choi & R Jude Samulski

Published online: 09 November 2006 | doi:10.1038/nprot.2006.207

Abstract - | Full Text - Production and characterization of adeno-associated viral vectors | PDF (548 KB) - Production and characterization of adeno-associated viral vectors

Whole-body imaging with fluorescent proteins - pp1429 - 1438

Robert M Hoffman & Meng Yang

Published online: 02 November 2006 | doi:10.1038/nprot.2006.223

Abstract - | Full Text - Whole-body imaging with fluorescent proteins | PDF (677 KB) - Whole-body imaging with fluorescent proteins

Preparation of organotypic hippocampal slice cultures: interface method - pp1439 - 1445

Anna De Simoni & Lily MY Yu

Published online: 02 November 2006 | doi:10.1038/nprot.2006.228

Abstract - | Full Text - Preparation of organotypic hippocampal slice cultures: interface method | PDF (280 KB) - Preparation of organotypic hippocampal slice cultures: interface method | Supplementary information

See also: Protocol by Davie et al. | Protocol by Gogolla et al. | Protocol by Gogolla et al. | Protocol by O'Brien & Lummis

Protein profiling by the combination of two independent mass spectrometry techniques - pp1446 - 1452

Wei-Qiang Chen, Sung-Ung Kang & Gert Lubec

Published online: 02 November 2006 | doi:10.1038/nprot.2006.246

Abstract - | Full Text - Protein profiling by the combination of two independent mass spectrometry techniques | PDF (464 KB) - Protein profiling by the combination of two independent mass spectrometry techniques | Supplementary information

Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes - pp1453 - 1457

Seiryo Sugiura, Satoshi Nishimura, Soichiro Yasuda, Yumiko Hosoya & Kaoru Katoh

Published online: 02 November 2006 | doi:10.1038/nprot.2006.241

Abstract - | Full Text - Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes | PDF (196 KB) - Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes | Supplementary information

Analysis of apoptosis by propidium iodide staining and flow cytometry - pp1458 - 1461

Carlo Riccardi & Ildo Nicoletti

Published online: 09 November 2006 | doi:10.1038/nprot.2006.238

Abstract - | Full Text - Analysis of apoptosis by propidium iodide staining and flow cytometry | PDF (165 KB) - Analysis of apoptosis by propidium iodide staining and flow cytometry

In situ hybridization for mRNA detection in Arabidopsis tissue sections - pp1462 - 1467

Philip B Brewer, Marcus G Heisler, Jan Hejátko, Jií Friml & Eva Benková

Published online: 09 November 2006 | doi:10.1038/nprot.2006.226

Abstract - | Full Text - In situ hybridization for mRNA detection in Arabidopsis tissue sections | PDF (285 KB) - In situ hybridization for mRNA detection in Arabidopsis tissue sections

The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree - pp1468 - 1476

John Isaac Murray, Zhirong Bao, Thomas J Boyle & Robert H Waterston

Published online: 09 November 2006 | doi:10.1038/nprot.2006.222

Abstract - | Full Text - The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree | PDF (811 KB) - The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree | Supplementary information

Methods for MAG3 conjugation and ^99mTc radiolabeling of biomolecules - pp1477 - 1480

Yi Wang, Guozheng Liu & Donald J Hnatowich

Published online: 09 November 2006 | doi:10.1038/nprot.2006.262

Abstract - | Full Text - Methods for MAG3 conjugation and 99mTc radiolabeling of biomolecules | PDF (149 KB) - Methods for MAG3 conjugation and 99mTc radiolabeling of biomolecules

Serial analysis of binding elements for human transcription factors - pp1481 - 1493

Jiguo Chen

Published online: 09 November 2006 | doi:10.1038/nprot.2006.237

Abstract - | Full Text - Serial analysis of binding elements for human transcription factors | PDF (259 KB) - Serial analysis of binding elements for human transcription factors

Tissue-specific and cell type–specific RNA interference in vivo - pp1494 - 1501

Manjeet K Rao & Miles F Wilkinson

Published online: 09 November 2006 | doi:10.1038/nprot.2006.260

Abstract - | Full Text - Tissue-specific and cell type–specific RNA interference in vivo | PDF (279 KB) - Tissue-specific and cell type–specific RNA interference in vivo

An ex vivo method for rapid generation of monoclonal antibodies (ADLib system) - pp1502 - 1506

Hidetaka Seo, Shu-ichi Hashimoto, Kyoko Tsuchiya, Waka Lin, Takehiko Shibata & Kunihiro Ohta

Published online: 09 November 2006 | doi:10.1038/nprot.2006.248

Abstract - | Full Text - An ex vivo method for rapid generation of monoclonal antibodies (ADLib system) | PDF (183 KB) - An ex vivo method for rapid generation of monoclonal antibodies (ADLib system)

Intracellular cytokine optimization and standard operating procedure - pp1507 - 1516

Laurie Lamoreaux, Mario Roederer & Richard Koup

Published online: 09 November 2006 | doi:10.1038/nprot.2006.268

Abstract - | Full Text - Intracellular cytokine optimization and standard operating procedure | PDF (417 KB) - Intracellular cytokine optimization and standard operating procedure

Diolistic labeling of neuronal cultures and intact tissue using a hand-held gene gun - pp1517 - 1521

John A O'Brien & Sarah C R Lummis

Published online: 09 November 2006 | doi:10.1038/nprot.2006.258

Abstract - | Full Text - Diolistic labeling of neuronal cultures and intact tissue using a hand-held gene gun | PDF (419 KB) - Diolistic labeling of neuronal cultures and intact tissue using a hand-held gene gun

See also: Protocol by Davie et al. | Protocol by Gogolla et al. | Protocol by Gogolla et al. | Protocol by De Simoni & MY Yu

Quality assurance for polychromatic flow cytometry - pp1522 - 1530

Stephen P Perfetto, David Ambrozak, Richard Nguyen, Pratip Chattopadhyay & Mario Roederer

Published online: 09 November 2006 | doi:10.1038/nprot.2006.250

Abstract - | Full Text - Quality assurance for polychromatic flow cytometry | PDF (286 KB) - Quality assurance for polychromatic flow cytometry

Analysis of translation initiation using a translation control reporter system - pp1531 - 1537

Volker Wiesenthal, Achim Leutz & Cornelis F Calkhoven

Published online: 09 November 2006 | doi:10.1038/nprot.2006.274

Abstract - | Full Text - Analysis of translation initiation using a translation control reporter system | PDF (335 KB) - Analysis of translation initiation using a translation control reporter system

Two-step metal affinity purification of double-tagged (NusA–His₆) fusion proteins - pp1538 - 1543

Ario de Marco

Published online: 09 November 2006 | doi:10.1038/nprot.2006.289

Abstract - | Full Text - Two-step metal affinity purification of double-tagged (NusA–His6) fusion proteins | PDF (245 KB) - Two-step metal affinity purification of double-tagged (NusA–His6) fusion proteins

Negative detection of biomolecules separated in polyacrylamide electrophoresis gels - pp1544 - 1551

Lila Castellanos-Serra & Eugenio Hardy

Published online: 09 November 2006 | doi:10.1038/nprot.2006.233

Abstract - | Full Text - Negative detection of biomolecules separated in polyacrylamide electrophoresis gels | PDF (219 KB) - Negative detection of biomolecules separated in polyacrylamide electrophoresis gels

In vivo electroporation in the embryonic mouse central nervous system - pp1552 - 1558

Tetsuichiro Saito

Published online: 09 November 2006 | doi:10.1038/nprot.2006.276

Abstract - | Full Text - In vivo electroporation in the embryonic mouse central nervous system | PDF (501 KB) - In vivo electroporation in the embryonic mouse central nervous system

Quantification of mRNA using real-time RT-PCR - pp1559 - 1582

Tania Nolan, Rebecca E Hands & Stephen A Bustin

Published online: 09 November 2006 | doi:10.1038/nprot.2006.236

Abstract - | Full Text - Quantification of mRNA using real-time RT-PCR | PDF (972 KB) - Quantification of mRNA using real-time RT-PCR

Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol - pp1583 - 1590

Brittney-Shea Herbert, Amelia E Hochreiter, Woodring E Wright & Jerry W Shay

Published online: 09 November 2006 | doi:10.1038/nprot.2006.239

Abstract - | Full Text - Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol | PDF (318 KB) - Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain - pp1591 - 1595

Donna M Wilcock, Marcia N Gordon & Dave Morgan

Published online: 09 November 2006 | doi:10.1038/nprot.2006.277

Abstract - | Full Text - Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain | PDF (255 KB) - Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain

A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction - pp1596 - 1609

Ngan F Huang, Richard E Sievers, Jennifer S Park, Qizhi Fang, Song Li & Randall J Lee

Published online: 09 November 2006 | doi:10.1038/nprot.2006.188

Abstract - | Full Text - A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction | PDF (715 KB) - A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction

Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution - pp1610 - 1616

Kevin A Wilkinson, Edward J Merino & Kevin M Weeks

Published online: 09 November 2006 | doi:10.1038/nprot.2006.249

Abstract - | Full Text - Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution | PDF (235 KB) - Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution

RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions - pp1617 - 1620

Ben Lehner, Julia Tischler & Andrew G Fraser

Published online: 09 November 2006 | doi:10.1038/nprot.2006.245

Abstract - | Full Text - RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions | PDF (163 KB) - RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions

Methylation-specific digital karyotyping - pp1621 - 1636

Min Hu, Jun Yao & Kornelia Polyak

Published online: 09 November 2006 | doi:10.1038/nprot.2006.278

Abstract - | Full Text - Methylation-specific digital karyotyping | PDF (477 KB) - Methylation-specific digital karyotyping

Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly - pp1637 - 1652

David A Wright, Stacey Thibodeau-Beganny, Jeffry D Sander, Ronnie J Winfrey, Andrew S Hirsh, Magdalena Eichtinger, Fengli Fu, Matthew H Porteus, Drena Dobbs, Daniel F Voytas & J Keith Joung

Published online: 16 November 2006 | doi:10.1038/nprot.2006.259

Abstract - | Full Text - Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly | PDF (528 KB) - Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly | Supplementary information