Protocol abstract
Nature Protocols 1, - 1429 - 1438 (2006)
Published online: 2 November 2006 | doi:10.1038/nprot.2006.223
Subject Categories: Cell and tissue culture | Genetic modification | Imaging | Model organisms
Whole-body imaging with fluorescent proteins
Robert M Hoffman1 & Meng Yang1
Abstract
The intrinsic brightness of fluorescent proteins has been taken advantage of to develop a technology of whole-body imaging of tumors and gene expression in mouse internal organs. Stable transformation with fluorescent protein genes can be effected using retroviral vectors containing a selectable marker such as neomycin resistance. The cells that stably express fluorescent proteins can then be transplanted into appropriate mouse models. For whole-body imaging, nude mice are very appropriate. If wild-type mice are used, then hair must be removed by shaving or depilation. The instruments used can range from a simple LED flashlight and appropriate excitation and emission filters to sophisticated equipment such as the Olympus OV100 with a wide range of magnification, enabling both macroimaging and microimaging. It is crucial that proper filters be used such that background autofluorescence is minimal. Fluorescent protein–based imaging technology can be used for whole-body imaging of fluorescent cells on essentially all organs. The timeline for these experiments varies from 2 days to 2 months.
- AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, USA.
Correspondence to: Robert M Hoffman1 e-mail: all@anticancer.com
nature-products
A-Z product listing
- 1 ml 27G2 latex-free syringe(Becton Dickinson)
- 25
l Hamilton syringe(Fisher Scientific) - acepromazine maleate(Butler Animal Health Supply)
- Blue LED flashlight(LDP LLC)
- Cell(Olympus Biosystems)
- Cell lines, such as those derived from human cancer, to be transfected with genes coding for fluorescent proteins(American Type Culture Collection)
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