Protocol abstract
Nature Protocols 1, - 1596 - 1609 (2006)
Published online: 9 November 2006 | doi:10.1038/nprot.2006.188
Subject Categories: Model organisms | Pharmacology and toxicology
A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction
Ngan F Huang1, Richard E Sievers2, Jennifer S Park1, Qizhi Fang2, Song Li1,3 & Randall J Lee1,2
Abstract
We have developed a robust rat model of myocardial infarction (MI). Here we describe the step-by-step protocol for creating an ischemia-reperfusion rat model of MI. We also describe how to deliver therapeutic injections of mesenchymal stem cells (MSCs) together with fibrin, to show an application of this model. In addition, to confirm the presence of fibrin and cells in the infarct, visualization of MSCs and fibrin by histological techniques are also described. The ischemia-reperfusion MI model can be modified and generalized for use with various injectable polymers, cell types, drugs, DNA and combinations thereof. The model can be created in 7 days or less, depending on the timing of therapeutic intervention.
- University of California San Francisco and University of California Berkeley Joint Bioengineering Graduate Group, Berkeley, California, 94720-1762 USA.
- Department of Medicine, University of California San Francisco, San Francisco, California, 94143-1354 USA.
- Department of Bioengineering, University of California Berkeley, Berkeley, California, 94720-1762 USA.
Correspondence to: Randall J Lee1,2 e-mail: lee@medicine.ucsf.edu
nature-products
A-Z product listing
- 1.5 ml microcentrifuge tube(Eppendorf)
- 1.5M NaCl(Sigma)
- 100
20 mm cell culture dishes(BD Falcon) - 3-0 Silk suture(Genzyme Biosurgery)
- 4% Acetic acid(Sigma)
- 4-0 Dexon suture(Syneture)
