Differentiation of mouse embryonic stem cells to insulin-producing cells - pp495 - 507

Insa S Schroeder, Alexandra Rolletschek, Przemyslaw Blyszczuk, Gabriela Kania & Anna M Wobus

Published online: 29 June 2006 | doi:10.1038/nprot.2006.71

Abstract - | Full Text - Differentiation of mouse embryonic stem cells to insulin-producing cells | PDF (608 KB) - Differentiation of mouse embryonic stem cells to insulin-producing cells

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA - pp508 - 517

Mohammed Amarzguioui, Patric Lundberg, Edouard Cantin, James Hagstrom, Mark A Behlke & John J Rossi

Published online: 27 June 2006 | doi:10.1038/nprot.2006.72

Abstract - | Full Text - Rational design and in vitro and in vivo delivery of Dicer substrate siRNA | PDF (398 KB) - Rational design and in vitro and in vivo delivery of Dicer substrate siRNA

Southern blotting - pp518 - 525

Ed Southern

Published online: 27 June 2006 | doi:10.1038/nprot.2006.73

Abstract - | Full Text - Southern blotting | PDF (360 KB) - Southern blotting

Localized transfection with magnetic beads coated with PCR products and other nucleic acids - pp526 - 531

Maria Isabel Santori, Cayetano Gonzalez, Luis Serrano & Mark Isalan

Published online: 27 June 2006 | doi:10.1038/nprot.2006.74

Abstract - | Full Text - Localized transfection with magnetic beads coated with PCR products and other nucleic acids | PDF (342 KB) - Localized transfection with magnetic beads coated with PCR products and other nucleic acids | Supplementary information

Blind patch clamp recordings in embryonic and adult mammalian brain slices - pp532 - 542

David R Castañeda-Castellanos, Alexander C Flint & Arnold R Kriegstein

Published online: 27 June 2006 | doi:10.1038/nprot.2006.75

Abstract - | Full Text - Blind patch clamp recordings in embryonic and adult mammalian brain slices | PDF (567 KB) - Blind patch clamp recordings in embryonic and adult mammalian brain slices

See also: Protocol by Komai et al.

A lectin microarray approach for the rapid analysis of bacterial glycans - pp543 - 549

Ku-Lung Hsu & Lara K Mahal

Published online: 27 June 2006 | doi:10.1038/nprot.2006.76

Abstract - | Full Text - A lectin microarray approach for the rapid analysis of bacterial glycans | PDF (179 KB) - A lectin microarray approach for the rapid analysis of bacterial glycans

A detergent-based assay for the detection of promiscuous inhibitors - pp550 - 553

Brian Y Feng & Brian K Shoichet

Published online: 27 June 2006 | doi:10.1038/nprot.2006.77

Abstract - | Full Text - A detergent-based assay for the detection of promiscuous inhibitors | PDF (146 KB) - A detergent-based assay for the detection of promiscuous inhibitors

An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA - pp554 - 558

Yossi Weizmann, Zoya Cheglakov, Valeri Pavlov & Itamar Willner

Published online: 27 June 2006 | doi:10.1038/nprot.2006.78

Abstract - | Full Text - An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA | PDF (291 KB) - An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA

Analysis of locomotor activity rhythms in Drosophila - pp559 - 568

Ezio Rosato & Charalambos P Kyriacou

Published online: 06 July 2006 | doi:10.1038/nprot.2006.79

Abstract - | Full Text - Analysis of locomotor activity rhythms in Drosophila | PDF (476 KB) - Analysis of locomotor activity rhythms in Drosophila

A microarray-based protocol for monitoring the growth of yeast overexpression strains - pp569 - 576

Rebecca A Butcher & Stuart L Schreiber

Published online: 27 June 2006 | doi:10.1038/nprot.2006.80

Abstract - | Full Text - A microarray-based protocol for monitoring the growth of yeast overexpression strains | PDF (364 KB) - A microarray-based protocol for monitoring the growth of yeast overexpression strains

Immunoprecipitation of mRNA-protein complexes - pp577 - 580

Tiina Peritz, Fanyi Zeng, Theresa J Kannanayakal, Kalle Kilk, Emelía Eiríksdóttir, Ulo Langel & James Eberwine

Published online: 27 June 2006 | doi:10.1038/nprot.2006.82

Abstract - | Full Text - Immunoprecipitation of mRNA-protein complexes | PDF (129 KB) - Immunoprecipitation of mRNA-protein complexes

See also: Protocol by Zeng et al. | Protocol by Windbichler & Schroeder

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on - pp581 - 585

Piotr Chomczynski & Nicoletta Sacchi

Published online: 27 June 2006 | doi:10.1038/nprot.2006.83

Abstract - | Full Text - The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on | PDF (123 KB) - The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Laser-capture microdissection - pp586 - 603

Virginia Espina, Julia D Wulfkuhle, Valerie S Calvert, Amy VanMeter, Weidong Zhou, George Coukos, David H Geho, Emanuel F Petricoin, III & Lance A Liotta

Published online: 27 June 2006 | doi:10.1038/nprot.2006.85

Abstract - | Full Text - Laser-capture microdissection | PDF (519 KB) - Laser-capture microdissection

Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with ¹¹C - pp604 - 613

Qing Cheng, Sharon Stone-Elander & Elias S J Arnér

Published online: 06 July 2006 | doi:10.1038/nprot.2006.87

Abstract - | Full Text - Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C | PDF (342 KB) - Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C

See also: Protocol by Itsenko et al.

Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei - pp614 - 623

Chris J Janse, Blandine Franke-Fayard & Andrew P Waters

Published online: 06 July 2006 | doi:10.1038/nprot.2006.88

Abstract - | Full Text - Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei | PDF (342 KB) - Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei

High-throughput plasmid cDNA library screening - pp624 - 632

Kenneth H Wan, Charles Yu, Reed A George, Joseph W Carlson, Roger A Hoskins, Robert Svirskas, Mark Stapleton & Susan E Celniker

Published online: 06 July 2006 | doi:10.1038/nprot.2006.90

Abstract - | Full Text - High-throughput plasmid cDNA library screening | PDF (289 KB) - High-throughput plasmid cDNA library screening

Enhanced delivery of cell-penetrating peptide–peptide nucleic acid conjugates by endosomal disruption - pp633 - 636

Takehiko Shiraishi & Peter E Nielsen

Published online: 29 June 2006 | doi:10.1038/nprot.2006.92

Abstract - | Full Text - Enhanced delivery of cell-penetrating peptide–peptide nucleic acid conjugates by endosomal disruption | PDF (141 KB) - Enhanced delivery of cell-penetrating peptide–peptide nucleic acid conjugates by endosomal disruption

Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer - pp637 - 640

Nikolai Windbichler & Renée Schroeder

Published online: 27 June 2006 | doi:10.1038/nprot.2006.95

Abstract - | Full Text - Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer | PDF (184 KB) - Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer

See also: Protocol by Zeng et al. | Protocol by Peritz et al.

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method - pp641 - 646

Xiuren Zhang, Rossana Henriques, Shih-Shun Lin, Qi-Wen Niu & Nam-Hai Chua

Published online: 29 June 2006 | doi:10.1038/nprot.2006.97

Abstract - | Full Text - Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method | PDF (237 KB) - Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method

Two-photon targeted patching (TPTP) in vivo - pp647 - 652

Shoji Komai, Winfried Denk, Pavel Osten, Michael Brecht & Troy W Margrie

Published online: 27 June 2006 | doi:10.1038/nprot.2006.100

Abstract - | Full Text - Two-photon targeted patching (TPTP) in vivo | PDF (341 KB) - Two-photon targeted patching (TPTP) in vivo

See also: Protocol by Castañeda-Castellanos et al.

De novo production of antigen-specific suppressor cells in vivo - pp653 - 661

Karsten Kretschmer, Tracy S P Heng & Harald von Boehmer

Published online: 06 July 2006 | doi:10.1038/nprot.2006.105

Abstract - | Full Text - De novo production of antigen-specific suppressor cells in vivo | PDF (429 KB) - De novo production of antigen-specific suppressor cells in vivo | Supplementary information

Reverse engineering cellular networks - pp662 - 671

Adam A Margolin, Kai Wang, Wei Keat Lim, Manjunath Kustagi, Ilya Nemenman & Andrea Califano

Published online: 27 June 2006 | doi:10.1038/nprot.2006.106

Abstract - | Full Text - Reverse engineering cellular networks | PDF (562 KB) - Reverse engineering cellular networks | Supplementary information

The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis - pp672 - 682

David Usharauli, Ainhoa Perez-Diez & Polly Matzinger

Published online: 27 June 2006 | doi:10.1038/nprot.2006.107

Abstract - | Full Text - The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis | PDF (378 KB) - The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis

NMR: prediction of protein flexibility - pp683 - 688

Mark Berjanskii & David S Wishart

Published online: 06 July 2006 | doi:10.1038/nprot.2006.108

Abstract - | Full Text - NMR: prediction of protein flexibility | PDF (207 KB) - NMR: prediction of protein flexibility | Supplementary information

See also: Protocol by Tugarinov et al.

Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system - pp689 - 694

Nicolas Piganeau & Renée Schroeder

Published online: 06 July 2006 | doi:10.1038/nprot.2006.111

Abstract - | Full Text - Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system | PDF (229 KB) - Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system | Supplementary information

High Ca²⁺-phosphate transfection efficiency in low-density neuronal cultures - pp695 - 700

Min Jiang & Gong Chen

Published online: 06 July 2006 | doi:10.1038/nprot.2006.86

Abstract - | Full Text - High Ca2+-phosphate transfection efficiency in low-density neuronal cultures | PDF (457 KB) - High Ca2+-phosphate transfection efficiency in low-density neuronal cultures

Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles - pp701 - 706

James T Koerber, Narendra Maheshri, Brian K Kaspar & David V Schaffer

Published online: 06 July 2006 | doi:10.1038/nprot.2006.93

Abstract - | Full Text - Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles | PDF (183 KB) - Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles

Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins - pp707 - 713

Ganesan Vaidyanathan & Michael R Zalutsky

Published online: 06 July 2006 | doi:10.1038/nprot.2006.99

Abstract - | Full Text - Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins | PDF (219 KB) - Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins

Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro - pp714 - 719

Vadim V Demidov & Natalia E Broude

Published online: 06 July 2006 | doi:10.1038/nprot.2006.114

Abstract - | Full Text - Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro | PDF (204 KB) - Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro

Multiplex amplification of ancient DNA - pp720 - 728

Holger Römpler, Paul H Dear, Johannes Krause, Matthias Meyer, Nadin Rohland, Torsten Schöneberg, Helen Spriggs, Mathias Stiller & Michael Hofreiter

Published online: 13 July 2006 | doi:10.1038/nprot.2006.84

Abstract - | Full Text - Multiplex amplification of ancient DNA | PDF (353 KB) - Multiplex amplification of ancient DNA

Chromatin immunoprecipitation and microarray-based analysis of protein location - pp729 - 748

Tong Ihn Lee, Sarah E Johnstone & Richard A Young

Published online: 13 July 2006 | doi:10.1038/nprot.2006.98

Abstract - | Full Text - Chromatin immunoprecipitation and microarray-based analysis of protein location | PDF (636 KB) - Chromatin immunoprecipitation and microarray-based analysis of protein location

Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy - pp749 - 754

Vitali Tugarinov, Voula Kanelis & Lewis E Kay

Published online: 13 July 2006 | doi:10.1038/nprot.2006.101

Abstract - | Full Text - Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy | PDF (481 KB) - Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy

Isolating and engineering human antibodies using yeast surface display - pp755 - 768

Ginger Chao, Wai L Lau, Benjamin J Hackel, Stephen L Sazinsky, Shaun M Lippow & K Dane Wittrup

Published online: 13 July 2006 | doi:10.1038/nprot.2006.94

Abstract - | Full Text - Isolating and engineering human antibodies using yeast surface display | PDF (342 KB) - Isolating and engineering human antibodies using yeast surface display | Supplementary information

Sample extraction techniques for enhanced proteomic analysis of plant tissues - pp769 - 774

Tal Isaacson, Cynthia M B Damasceno, Ramu S Saravanan, Yonghua He, Carmen Catalá, Montserrat Saladié & Jocelyn K C Rose

Published online: 13 July 2006 | doi:10.1038/nprot.2006.102

Abstract - | Full Text - Sample extraction techniques for enhanced proteomic analysis of plant tissues | PDF (171 KB) - Sample extraction techniques for enhanced proteomic analysis of plant tissues

Subcellular imaging in the live mouse - pp775 - 782

Robert M Hoffman & Meng Yang

Published online: 27 July 2006 | doi:10.1038/nprot.2006.109

Abstract - | Full Text - Subcellular imaging in the live mouse | PDF (592 KB) - Subcellular imaging in the live mouse

Cross-species chromosome painting - pp783 - 790

Willem Rens, Beiyuan Fu, Patricia C M O'Brien & Malcolm Ferguson-Smith

Published online: 13 July 2006 | doi:10.1038/nprot.2006.91

Abstract - | Full Text - Cross-species chromosome painting | PDF (179 KB) - Cross-species chromosome painting

PCR-based accurate synthesis of long DNA sequences - pp791 - 797

Ai-Sheng Xiong, Quan-Hong Yao, Ri-He Peng, Hui Duan, Xian Li, Hui-Qin Fan, Zong-Ming Cheng & Yi Li

Published online: 13 July 2006 | doi:10.1038/nprot.2006.103

Abstract - | Full Text - PCR-based accurate synthesis of long DNA sequences | PDF (260 KB) - PCR-based accurate synthesis of long DNA sequences

Labeling of aliphatic carboxylic acids using [¹¹C]carbon monoxide - pp798 - 802

Oleksiy Itsenko, Tor Kihlberg & Bengt Långström

Published online: 20 July 2006 | doi:10.1038/nprot.2006.112

Abstract - | Full Text - Labeling of aliphatic carboxylic acids using [11C]carbon monoxide | PDF (168 KB) - Labeling of aliphatic carboxylic acids using [11C]carbon monoxide

See also: Protocol by Cheng et al.

Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2 - pp803 - 811

Arkadiusz Welman, Jane Barraclough & Caroline Dive

Published online: 20 July 2006 | doi:10.1038/nprot.2006.117

Abstract - | Full Text - Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2 | PDF (265 KB) - Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2

State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research - pp812 - 823

Odile Carrette, Pierre R Burkhard, Jean-Charles Sanchez & Denis F Hochstrasser

Published online: 20 July 2006 | doi:10.1038/nprot.2006.104

Abstract - | Full Text - State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research | PDF (1,733 KB) - State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging - pp824 - 827

Evan W Miller, Li Zeng, Dylan W Domaille & Christopher J Chang

Published online: 27 July 2006 | doi:10.1038/nprot.2006.140

Abstract - | Full Text - Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging | PDF (212 KB) - Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging

Alignment and calibration of a focal neurotransmitter uncaging system - pp828 - 832

Dmitry V Sarkisov & Samuel S-H Wang

Published online: 27 July 2006 | doi:10.1038/nprot.2006.124

Abstract - | Full Text - Alignment and calibration of a focal neurotransmitter uncaging system | PDF (231 KB) - Alignment and calibration of a focal neurotransmitter uncaging system

A rat model for neural circuitry abnormalities in schizophrenia - pp833 - 839

Sabina Berretta & Francine M Benes

Published online: 27 July 2006 | doi:10.1038/nprot.2006.110

Abstract - | Full Text - A rat model for neural circuitry abnormalities in schizophrenia | PDF (319 KB) - A rat model for neural circuitry abnormalities in schizophrenia

A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals - pp840 - 847

Herman Meurs, Ruud E Santing, René Remie, Thomas W van der Mark, Fiona J Westerhof, Annet B Zuidhof, I Sophie T Bos & Johan Zaagsma

Published online: 27 July 2006 | doi:10.1038/nprot.2006.144

Abstract - | Full Text - A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals | PDF (322 KB) - A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals

Morris water maze: procedures for assessing spatial and related forms of learning and memory - pp848 - 858

Charles V Vorhees & Michael T Williams

Published online: 27 July 2006 | doi:10.1038/nprot.2006.116

Abstract - | Full Text - Morris water maze: procedures for assessing spatial and related forms of learning and memory | PDF (222 KB) - Morris water maze: procedures for assessing spatial and related forms of learning and memory

See also: Protocol by Deacon

Flow cytometry after bromodeoxyuridine labeling to measure S and G₂+M phase durations plus doubling times in vitro and in vivo - pp859 - 869

Nicholas HA Terry & R Allen White

Published online: 27 July 2006 | doi:10.1038/nprot.2006.113

Abstract - | Full Text - Flow cytometry after bromodeoxyuridine labeling to measure S and G2+M phase durations plus doubling times in vitro and in vivo | PDF (362 KB) - Flow cytometry after bromodeoxyuridine labeling to measure S and G2+M phase durations plus doubling times in vitro and in vivo

Isolation of DNA from bacterial samples of the human gastrointestinal tract - pp870 - 873

Erwin G Zoetendal, Hans GHJ Heilig, Eline S Klaassens, Carien CGM Booijink, Michiel Kleerebezem, Hauke Smidt & Willem M de Vos

Published online: 27 July 2006 | doi:10.1038/nprot.2006.142

Abstract - | Full Text - Isolation of DNA from bacterial samples of the human gastrointestinal tract | PDF (135 KB) - Isolation of DNA from bacterial samples of the human gastrointestinal tract

Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues - pp874 - 879

Isabelle Schwartz-Cornil, Mathieu Epardaud & Michel Bonneau

Published online: 03 August 2006 | doi:10.1038/nprot.2006.147

Abstract - | Full Text - Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues | PDF (215 KB) - Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues

Automated serum peptide profiling - pp880 - 891

Josep Villanueva, Kevin Lawlor, Ricardo Toledo-Crow & Paul Tempst

Published online: 27 July 2006 | doi:10.1038/nprot.2006.128

Abstract - | Full Text - Automated serum peptide profiling | PDF (861 KB) - Automated serum peptide profiling | Supplementary information

Clinical and empirical applications of the Rey–Osterrieth Complex Figure Test - pp892 - 899

Min-Sup Shin, Sun-Young Park, Se-Ran Park, Soon-Ho Seol & Jun Soo Kwon

Published online: 27 July 2006 | doi:10.1038/nprot.2006.115

Abstract - | Full Text - Clinical and empirical applications of the Rey–Osterrieth Complex Figure Test | PDF (168 KB) - Clinical and empirical applications of the Rey–Osterrieth Complex Figure Test

Construction of marker-free transplastomic tobacco using the Cre-loxP site-specific recombination system - pp900 - 910

Kerry Ann Lutz, Zora Svab & Pal Maliga

Published online: 03 August 2006 | doi:10.1038/nprot.2006.118

Abstract - | Full Text - Construction of marker-free transplastomic tobacco using the Cre-loxP site-specific recombination system | PDF (326 KB) - Construction of marker-free transplastomic tobacco using the Cre-loxP site-specific recombination system

Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices - pp911 - 919

Ken-Ichi Okamoto & Yasunori Hayashi

Published online: 03 August 2006 | doi:10.1038/nprot.2006.122

Abstract - | Full Text - Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices | PDF (338 KB) - Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices

A protocol for PAIR: PNA-assisted identification of RNA binding proteins in living cells - pp920 - 927

Fanyi Zeng, Tiina Peritz, Theresa J Kannanayakal, Kalle Kilk, Emelía Eiríksdóttir, Ulo Langel & James Eberwine

Published online: 03 August 2006 | doi:10.1038/nprot.2006.81

Abstract - | Full Text - A protocol for PAIR: PNA-assisted identification of RNA binding proteins in living cells | PDF (235 KB) - A protocol for PAIR: PNA-assisted identification of RNA binding proteins in living cells | Supplementary information

See also: Protocol by Peritz et al. | Protocol by Windbichler & Schroeder

Color-coded fluorescence imaging of tumor-host interactions - pp928 - 935

Robert M Hoffman & Meng Yang

Published online: 03 August 2006 | doi:10.1038/nprot.2006.119

Abstract - | Full Text - Color-coded fluorescence imaging of tumor-host interactions | PDF (484 KB) - Color-coded fluorescence imaging of tumor-host interactions | Supplementary information

Housing, husbandry and handling of rodents for behavioral experiments - pp936 - 946

Robert M J Deacon

Published online: 03 August 2006 | doi:10.1038/nprot.2006.120

Abstract - | Full Text - Housing, husbandry and handling of rodents for behavioral experiments | PDF (402 KB) - Housing, husbandry and handling of rodents for behavioral experiments

See also: Protocol by Vorhees & Williams

Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed - pp947 - 953

Maria E Bulina, Konstantin A Lukyanov, Olga V Britanova, Daria Onichtchouk, Sergey Lukyanov & Dmitriy M Chudakov

Published online: 03 August 2006 | doi:10.1038/nprot.2006.89

Abstract - | Full Text - Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed | PDF (274 KB) - Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed

Isolation of RNA from bacterial samples of the human gastrointestinal tract - pp954 - 959

Erwin G Zoetendal, Carien CGM Booijink, Eline S Klaassens, Hans GHJ Heilig, Michiel Kleerebezem, Hauke Smidt & Willem M de Vos

Published online: 03 August 2006 | doi:10.1038/nprot.2006.143

Abstract - | Full Text - Isolation of RNA from bacterial samples of the human gastrointestinal tract | PDF (165 KB) - Isolation of RNA from bacterial samples of the human gastrointestinal tract

Cell tracking using a photoconvertible fluorescent protein - pp960 - 967

Kohei Hatta, Hitomi Tsujii & Tomomi Omura

Published online: 03 August 2006 | doi:10.1038/nprot.2006.96

Abstract - | Full Text - Cell tracking using a photoconvertible fluorescent protein | PDF (452 KB) - Cell tracking using a photoconvertible fluorescent protein | Supplementary information

Assay of protein kinases using radiolabeled ATP: a protocol - pp968 - 971

C James Hastie, Hilary J McLauchlan & Philip Cohen

Published online: 03 August 2006 | doi:10.1038/nprot.2006.149

Abstract - | Full Text - Assay of protein kinases using radiolabeled ATP: a protocol | PDF (118 KB) - Assay of protein kinases using radiolabeled ATP: a protocol

See also: Protocol by Brumbaugh et al.

Conjugation of DOTA-like chelating agents to peptides and radiolabeling with trivalent metallic isotopes - pp972 - 976

Jane K Sosabowski & Stephen J Mather

Published online: 03 August 2006 | doi:10.1038/nprot.2006.175

Abstract - | Full Text - Conjugation of DOTA-like chelating agents to peptides and radiolabeling with trivalent metallic isotopes | PDF (145 KB) - Conjugation of DOTA-like chelating agents to peptides and radiolabeling with trivalent metallic isotopes

Biolistic transfection of neuronal cultures using a hand-held gene gun - pp977 - 981

John A O'Brien & Sarah C R Lummis

Published online: 03 August 2006 | doi:10.1038/nprot.2006.145

Abstract - | Full Text - Biolistic transfection of neuronal cultures using a hand-held gene gun | PDF (356 KB) - Biolistic transfection of neuronal cultures using a hand-held gene gun

Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle - pp982 - 987

Jayne C Hope, Chris J Howard, Helen Prentice & Bryan Charleston

Published online: 03 August 2006 | doi:10.1038/nprot.2006.125

Abstract - | Full Text - Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle | PDF (292 KB) - Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle

An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy - pp988 - 994

Kevin J Darcy, Kevin Staras, Lucy M Collinson & Yukiko Goda

Published online: 10 August 2006 | doi:10.1038/nprot.2006.146

Abstract - | Full Text - An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy | PDF (452 KB) - An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy

Dendrimer-like DNA-based fluorescence nanobarcodes - pp995 - 1000

Soong Ho Um, Jong Bum Lee, Sang Yeon Kwon, Yougen Li & Dan Luo

Published online: 10 August 2006 | doi:10.1038/nprot.2006.141

Abstract - | Full Text - Dendrimer-like DNA-based fluorescence nanobarcodes | PDF (277 KB) - Dendrimer-like DNA-based fluorescence nanobarcodes

Studying the uptake of cell-penetrating peptides - pp1001 - 1005

Tina Holm, Henrik Johansson, Pontus Lundberg, Margus Pooga, Maria Lindgren & Ülo Langel

Published online: 10 August 2006 | doi:10.1038/nprot.2006.174

Abstract - | Full Text - Studying the uptake of cell-penetrating peptides | PDF (202 KB) - Studying the uptake of cell-penetrating peptides

Production of recombinant protein in Pichia pastoris by fermentation - pp1006 - 1021

Berend Tolner, Lisa Smith, Richard H J Begent & Kerry A Chester

Published online: 08 August 2006 | doi:10.1038/nprot.2006.126

Abstract - | Full Text - Production of recombinant protein in Pichia pastoris by fermentation | PDF (333 KB) - Production of recombinant protein in Pichia pastoris by fermentation | Supplementary information

A double-reporter splicing assay for determining splicing efficiency in mammalian cells - pp1022 - 1028

Md. Talat Nasim & Ian C Eperon

Published online: 10 August 2006 | doi:10.1038/nprot.2006.148

Abstract - | Full Text - A double-reporter splicing assay for determining splicing efficiency in mammalian cells | PDF (188 KB) - A double-reporter splicing assay for determining splicing efficiency in mammalian cells

Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes - pp1029 - 1043

Julie M Pratt, Deborah M Simpson, Mary K Doherty, Jenny Rivers, Simon J Gaskell & Robert J Beynon

Published online: 17 August 2006 | doi:10.1038/nprot.2006.129

Abstract - | Full Text - Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes | PDF (684 KB) - Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes

Single- and dual-parameter FRET kinase probes based on pleckstrin - pp1044 - 1055

Justin Brumbaugh, Andreas Schleifenbaum, Gunter Stier, Michael Sattler & Carsten Schultz

Published online: 17 August 2006 | doi:10.1038/nprot.2006.177

Abstract - | Full Text - Single- and dual-parameter FRET kinase probes based on pleckstrin | PDF (274 KB) - Single- and dual-parameter FRET kinase probes based on pleckstrin

See also: Protocol by Hastie et al.