Volume 1 Issue 2, August 2006

Four-dimensional imaging of convergent-extension movement of neutral plate and organogenesis derived from placodes in the zebrafish embryo. From the protocol by Hatta et al., which describes cell tracking using a photoconvertible fluorescent protein. Cover by Erin Boyle.

Protocol

Differentiation of mouse embryonic stem cells to insulin-producing cells

Insa S Schroeder
Alexandra Rolletschek
Anna M Wobus
Protocol 29 Jun 2006
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Rational design and in vitro and in vivo delivery of Dicer substrate siRNA

Mohammed Amarzguioui
Patric Lundberg
John J Rossi
Protocol 27 Jun 2006
Southern blotting

Ed Southern
Protocol 27 Jun 2006
Localized transfection with magnetic beads coated with PCR products and other nucleic acids

Maria Isabel Santori
Cayetano Gonzalez
Mark Isalan
Protocol 27 Jun 2006
Blind patch clamp recordings in embryonic and adult mammalian brain slices

David R Castañeda-Castellanos
Alexander C Flint
Arnold R Kriegstein
Protocol 27 Jun 2006
A lectin microarray approach for the rapid analysis of bacterial glycans

Ku-Lung Hsu
Lara K Mahal
Protocol 27 Jun 2006
A detergent-based assay for the detection of promiscuous inhibitors

Brian Y Feng
Brian K Shoichet
Protocol 27 Jun 2006
An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA

Yossi Weizmann
Zoya Cheglakov
Itamar Willner
Protocol 27 Jun 2006
Analysis of locomotor activity rhythms in Drosophila

Ezio Rosato
Charalambos P Kyriacou

Collection:

2017 Nobel Prize in Physiology or Medicine
Protocol 06 Jul 2006
A microarray-based protocol for monitoring the growth of yeast overexpression strains

Rebecca A Butcher
Stuart L Schreiber
Protocol 27 Jun 2006
Immunoprecipitation of mRNA-protein complexes

Tiina Peritz
Fanyi Zeng
James Eberwine
Protocol 27 Jun 2006
The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Piotr Chomczynski
Nicoletta Sacchi
Protocol 27 Jun 2006
Laser-capture microdissection

Virginia Espina
Julia D Wulfkuhle
Lance A Liotta
Protocol 27 Jun 2006
Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with ¹¹C

Qing Cheng
Sharon Stone-Elander
Elias S J Arnér
Protocol 06 Jul 2006
Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei

Chris J Janse
Blandine Franke-Fayard
Andrew P Waters
Protocol 06 Jul 2006
High-throughput plasmid cDNA library screening

Kenneth H Wan
Charles Yu
Susan E Celniker
Protocol 06 Jul 2006
Enhanced delivery of cell-penetrating peptide–peptide nucleic acid conjugates by endosomal disruption

Takehiko Shiraishi
Peter E Nielsen
Protocol 29 Jun 2006
Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer

Nikolai Windbichler
Renée Schroeder
Protocol 27 Jun 2006
Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method

Xiuren Zhang
Rossana Henriques
Nam-Hai Chua
Protocol 29 Jun 2006
Two-photon targeted patching (TPTP) in vivo

Shoji Komai
Winfried Denk
Troy W Margrie
Protocol 27 Jun 2006
De novo production of antigen-specific suppressor cells in vivo

Karsten Kretschmer
Tracy S P Heng
Harald von Boehmer
Protocol 06 Jul 2006
Reverse engineering cellular networks

Adam A Margolin
Kai Wang
Andrea Califano
Protocol 27 Jun 2006
The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis

David Usharauli
Ainhoa Perez-Diez
Polly Matzinger
Protocol 27 Jun 2006
NMR: prediction of protein flexibility

Mark Berjanskii
David S Wishart
Protocol 06 Jul 2006
Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system

Nicolas Piganeau
Renée Schroeder
Protocol 06 Jul 2006
High Ca²⁺-phosphate transfection efficiency in low-density neuronal cultures

Min Jiang
Gong Chen
Protocol 06 Jul 2006
Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles

James T Koerber
Narendra Maheshri
David V Schaffer
Protocol 06 Jul 2006
Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins

Ganesan Vaidyanathan
Michael R Zalutsky
Protocol 06 Jul 2006
Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro

Vadim V Demidov
Natalia E Broude
Protocol 06 Jul 2006
Multiplex amplification of ancient DNA

Holger Römpler
Paul H Dear
Michael Hofreiter
Protocol 13 Jul 2006
Chromatin immunoprecipitation and microarray-based analysis of protein location

Tong Ihn Lee
Sarah E Johnstone
Richard A Young
Protocol 13 Jul 2006
Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy

Vitali Tugarinov
Voula Kanelis
Lewis E Kay
Protocol 13 Jul 2006
Isolating and engineering human antibodies using yeast surface display

Ginger Chao
Wai L Lau
K Dane Wittrup
Protocol 13 Jul 2006
Sample extraction techniques for enhanced proteomic analysis of plant tissues

Tal Isaacson
Cynthia M B Damasceno
Jocelyn K C Rose
Protocol 13 Jul 2006
Subcellular imaging in the live mouse

Robert M Hoffman
Meng Yang
Protocol 27 Jul 2006
Cross-species chromosome painting

Willem Rens
Beiyuan Fu
Malcolm Ferguson-Smith
Protocol 13 Jul 2006
PCR-based accurate synthesis of long DNA sequences

Ai-Sheng Xiong
Quan-Hong Yao
Yi Li
Protocol 13 Jul 2006
Labeling of aliphatic carboxylic acids using [¹¹C]carbon monoxide

Oleksiy Itsenko
Tor Kihlberg
Bengt Långström
Protocol 20 Jul 2006
Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2

Arkadiusz Welman
Jane Barraclough
Caroline Dive
Protocol 20 Jul 2006
State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

Odile Carrette
Pierre R Burkhard
Denis F Hochstrasser
Protocol 20 Jul 2006
Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging

Evan W Miller
Li Zeng
Christopher J Chang
Protocol 27 Jul 2006
Alignment and calibration of a focal neurotransmitter uncaging system

Dmitry V Sarkisov
Samuel S-H Wang
Protocol 27 Jul 2006
A rat model for neural circuitry abnormalities in schizophrenia

Sabina Berretta
Francine M Benes
Protocol 27 Jul 2006
A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals

Herman Meurs
Ruud E Santing
Johan Zaagsma
Protocol 27 Jul 2006
Morris water maze: procedures for assessing spatial and related forms of learning and memory

Charles V Vorhees
Michael T Williams
Protocol 27 Jul 2006
Flow cytometry after bromodeoxyuridine labeling to measure S and G₂+M phase durations plus doubling times in vitro and in vivo

Nicholas HA Terry
R Allen White
Protocol 27 Jul 2006
Isolation of DNA from bacterial samples of the human gastrointestinal tract

Erwin G Zoetendal
Hans GHJ Heilig
Willem M de Vos
Protocol 27 Jul 2006
Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues

Isabelle Schwartz-Cornil
Mathieu Epardaud
Michel Bonneau
Protocol 03 Aug 2006
Automated serum peptide profiling

Josep Villanueva
Kevin Lawlor
Paul Tempst
Protocol 27 Jul 2006
Clinical and empirical applications of the Rey–Osterrieth Complex Figure Test

Min-Sup Shin
Sun-Young Park
Jun Soo Kwon
Protocol 27 Jul 2006
Construction of marker-free transplastomic tobacco using the Cre-loxP site-specific recombination system

Kerry Ann Lutz
Zora Svab
Pal Maliga
Protocol 03 Aug 2006
Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices

Ken-Ichi Okamoto
Yasunori Hayashi
Protocol 03 Aug 2006
A protocol for PAIR: PNA-assisted identification of RNA binding proteins in living cells

Fanyi Zeng
Tiina Peritz
James Eberwine
Protocol 03 Aug 2006
Color-coded fluorescence imaging of tumor-host interactions

Robert M Hoffman
Meng Yang
Protocol 03 Aug 2006
Housing, husbandry and handling of rodents for behavioral experiments

Robert M J Deacon
Protocol 03 Aug 2006
Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed

Maria E Bulina
Konstantin A Lukyanov
Dmitriy M Chudakov
Protocol 03 Aug 2006
Isolation of RNA from bacterial samples of the human gastrointestinal tract

Erwin G Zoetendal
Carien CGM Booijink
Willem M de Vos
Protocol 03 Aug 2006
Cell tracking using a photoconvertible fluorescent protein

Kohei Hatta
Hitomi Tsujii
Tomomi Omura
Protocol 03 Aug 2006
Assay of protein kinases using radiolabeled ATP: a protocol

C James Hastie
Hilary J McLauchlan
Philip Cohen
Protocol 03 Aug 2006
Conjugation of DOTA-like chelating agents to peptides and radiolabeling with trivalent metallic isotopes

Jane K Sosabowski
Stephen J Mather
Protocol 03 Aug 2006
Biolistic transfection of neuronal cultures using a hand-held gene gun

John A O'Brien
Sarah C R Lummis
Protocol 03 Aug 2006
Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle

Jayne C Hope
Chris J Howard
Bryan Charleston
Protocol 03 Aug 2006
An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy

Kevin J Darcy
Kevin Staras
Yukiko Goda
Protocol 10 Aug 2006
Dendrimer-like DNA-based fluorescence nanobarcodes

Soong Ho Um
Jong Bum Lee
Dan Luo
Protocol 10 Aug 2006
Studying the uptake of cell-penetrating peptides

Tina Holm
Henrik Johansson
Ülo Langel
Protocol 10 Aug 2006
Production of recombinant protein in Pichia pastoris by fermentation

Berend Tolner
Lisa Smith
Kerry A Chester
Protocol 08 Aug 2006
A double-reporter splicing assay for determining splicing efficiency in mammalian cells

Md. Talat Nasim
Ian C Eperon
Protocol 10 Aug 2006
Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes

Julie M Pratt
Deborah M Simpson
Robert J Beynon
Protocol 17 Aug 2006
Single- and dual-parameter FRET kinase probes based on pleckstrin

Justin Brumbaugh
Andreas Schleifenbaum
Carsten Schultz
Protocol 17 Aug 2006

Volume 1 Issue 2, August 2006

Protocol

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