Live-cell assay to detect antigen-specific CD4⁺ T-cell responses by CD154 expression - pp1 - 6

Pratip K Chattopadhyay, Joanne Yu & Mario Roederer

Published online: 12 May 2006 | doi:10.1038/nprot.2006.1

Abstract - | Full Text - Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression | PDF (785 KB) - Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression

T-maze alternation in the rodent - pp7 - 12

Robert M J Deacon & J Nicholas P Rawlins

Published online: 27 June 2006 | doi:10.1038/nprot.2006.2

Abstract - | Full Text - T-maze alternation in the rodent | PDF (146 KB) - T-maze alternation in the rodent

See also: Protocol by Deacon | Protocol by Deacon | Protocol by Deacon

Appetitive position discrimination in the T-maze - pp13 - 15

Robert M J Deacon

Published online: 27 June 2006 | doi:10.1038/nprot.2006.3

Abstract - | Full Text - Appetitive position discrimination in the T-maze | PDF (87 KB) - Appetitive position discrimination in the T-maze

See also: Protocol by Deacon & Rawlins | Protocol by Deacon | Protocol by Deacon

Tricine–SDS-PAGE - pp16 - 22

Hermann Schägger

Published online: 12 May 2006 | doi:10.1038/nprot.2006.4

Abstract - | Full Text - Tricine–SDS-PAGE | PDF (209 KB) - Tricine–SDS-PAGE

See also: Protocol by Wittig et al.

The comet assay: a method to measure DNA damage in individual cells - pp23 - 29

Peggy L Olive & Judit P Banáth

Published online: 27 June 2006 | doi:10.1038/nprot.2006.5

Abstract - | Full Text - The comet assay: a method to measure DNA damage in individual cells | PDF (307 KB) - The comet assay: a method to measure DNA damage in individual cells

See also: Protocol by van Genderen et al.

Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system - pp30 - 45

Xiangdong Meng & Scot A Wolfe

Published online: 27 June 2006 | doi:10.1038/nprot.2006.6

Abstract - | Full Text - Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system | PDF (727 KB) - Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system | Supplementary information

See also: Protocol by Bush et al. | Protocol by Palin et al. | Protocol by Nelson et al.

In-gel stable isotope labeling for relative quantification using mass spectrometry - pp46 - 51

John M Asara, Xiang Zhang, Bin Zheng, Lisa A Maroney, Heather R Christofk, Ning Wu & Lewis C Cantley

Published online: 27 June 2006 | doi:10.1038/nprot.2006.7

Abstract - | Full Text - In-gel stable isotope labeling for relative quantification using mass spectrometry | PDF (412 KB) - In-gel stable isotope labeling for relative quantification using mass spectrometry

See also: Protocol by Tanner et al. | Protocol by Shiio & Aebersold | Protocol by Burlina et al. | Protocol by Samyn et al.

Quantification of DNA methylation in electrofluidics chips (Bio-COBRA) - pp52 - 58

Romulo M Brena, Herbert Auer, Karl Kornacker & Christoph Plass

Published online: 27 June 2006 | doi:10.1038/nprot.2006.8

Abstract - | Full Text - Quantification of DNA methylation in electrofluidics chips (Bio-COBRA) | PDF (200 KB) - Quantification of DNA methylation in electrofluidics chips (Bio-COBRA)

Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform - pp59 - 66

Bruce S Edwards, Susan M Young, Tudor I Oprea, Cristian G Bologa, Eric R Prossnitz & Larry A Sklar

Published online: 27 June 2006 | doi:10.1038/nprot.2006.9

Abstract - | Full Text - Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform | PDF (349 KB) - Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform

Unrestrictive identification of post-translational modifications through peptide mass spectrometry - pp67 - 72

Stephen Tanner, Pavel A Pevzner & Vineet Bafna

Published online: 27 June 2006 | doi:10.1038/nprot.2006.10

Abstract - | Full Text - Unrestrictive identification of post-translational modifications through peptide mass spectrometry | PDF (201 KB) - Unrestrictive identification of post-translational modifications through peptide mass spectrometry

Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles - pp73 - 79

Mikael J Pittet, Filip K Swirski, Frederick Reynolds, Lee Josephson & Ralph Weissleder

Published online: 27 June 2006 | doi:10.1038/nprot.2006.11

Abstract - | Full Text - Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles | PDF (268 KB) - Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles

Cysteine engineering of polyproteins for single-molecule force spectroscopy - pp80 - 84

Hendrik Dietz, Morten Bertz, Michael Schlierf, Felix Berkemeier, Thomas Bornschlögl, Jan Philipp Junker & Matthias Rief

Published online: 27 June 2006 | doi:10.1038/nprot.2006.12

Abstract - | Full Text - Cysteine engineering of polyproteins for single-molecule force spectroscopy | PDF (199 KB) - Cysteine engineering of polyproteins for single-molecule force spectroscopy

The gelatin sponge–chorioallantoic membrane assay - pp85 - 91

Domenico Ribatti, Beatrice Nico, Angelo Vacca & Marco Presta

Published online: 27 June 2006 | doi:10.1038/nprot.2006.13

Abstract - | Full Text - The gelatin sponge–chorioallantoic membrane assay | PDF (606 KB) - The gelatin sponge–chorioallantoic membrane assay

Reverse MAPPIT detects disruptors of protein-protein interactions in human cells - pp92 - 97

Irma Lemmens, Sam Lievens, Sven Eyckerman & Jan Tavernier

Published online: 27 June 2006 | doi:10.1038/nprot.2006.14

Abstract - | Full Text - Reverse MAPPIT detects disruptors of protein-protein interactions in human cells | PDF (351 KB) - Reverse MAPPIT detects disruptors of protein-protein interactions in human cells

Immunocytochemical techniques for whole-mount in situ protein localization in plants - pp98 - 103

Michael Sauer, Tomasz Paciorek, Eva Benková & Jií Friml

Published online: 27 June 2006 | doi:10.1038/nprot.2006.15

Abstract - | Full Text - Immunocytochemical techniques for whole-mount in situ protein localization in plants | PDF (310 KB) - Immunocytochemical techniques for whole-mount in situ protein localization in plants

See also: Protocol by Paciorek et al.

Immunocytochemical technique for protein localization in sections of plant tissues - pp104 - 107

Tomasz Paciorek, Michael Sauer, Jozef Balla, Justyna Winiewska & Jií Friml

Published online: 27 June 2006 | doi:10.1038/nprot.2006.16

Abstract - | Full Text - Immunocytochemical technique for protein localization in sections of plant tissues | PDF (275 KB) - Immunocytochemical technique for protein localization in sections of plant tissues

See also: Protocol by Sauer et al.

Radiolabeling of HYNIC–annexin V with technetium-99m for in vivo imaging of apoptosis - pp108 - 110

Francis G Blankenberg, Jean-Luc Vanderheyden, H William Strauss & Jonathan F Tait

Published online: 27 June 2006 | doi:10.1038/nprot.2006.17

Abstract - | Full Text - Radiolabeling of HYNIC–annexin V with technetium-99m for in vivo imaging of apoptosis | PDF (116 KB) - Radiolabeling of HYNIC–annexin V with technetium-99m for in vivo imaging of apoptosis

See also: Protocol by Cooper et al.

Azido ruthenium: a new photoreactive probe for calcium-binding proteins - pp111 - 117

Adrian Israelson, Noam Zilberberg & Varda Shoshan-Barmatz

Published online: 27 June 2006 | doi:10.1038/nprot.2006.18

Abstract - | Full Text - Azido ruthenium: a new photoreactive probe for calcium-binding proteins | PDF (221 KB) - Azido ruthenium: a new photoreactive probe for calcium-binding proteins

See also: Protocol by Tovey et al.

Burrowing in rodents: a sensitive method for detecting behavioral dysfunction - pp118 - 121

Robert M J Deacon

Published online: 27 June 2006 | doi:10.1038/nprot.2006.19

Abstract - | Full Text - Burrowing in rodents: a sensitive method for detecting behavioral dysfunction | PDF (205 KB) - Burrowing in rodents: a sensitive method for detecting behavioral dysfunction | Supplementary information

See also: Protocol by Deacon & Rawlins | Protocol by Deacon | Protocol by Deacon

Digging and marble burying in mice: simple methods for in vivo identification of biological impacts - pp122 - 124

Robert M J Deacon

Published online: 27 June 2006 | doi:10.1038/nprot.2006.20

Abstract - | Full Text - Digging and marble burying in mice: simple methods for in vivo identification of biological impacts | PDF (84 KB) - Digging and marble burying in mice: simple methods for in vivo identification of biological impacts

See also: Protocol by Deacon & Rawlins | Protocol by Deacon | Protocol by Deacon

Production of cloned mice by somatic cellnuclear transfer - pp125 - 138

Satoshi Kishigami, Sayaka Wakayama, Nguyen Van Thuan, Hiroshi Ohta, Eiji Mizutani, Takafusa Hikichi, Hong-Thuy Bui, Sebastian Balbach, Atsuo Ogura, Michele Boiani & Teruhiko Wakayama

Published online: 27 June 2006 | doi:10.1038/nprot.2006.21

Abstract - | Full Text - Production of cloned mice by somatic cellnuclear transfer | PDF (834 KB) - Production of cloned mice by somatic cellnuclear transfer | Supplementary information

See also: Protocol by Singer et al.

Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry - pp139 - 145

Yuzuru Shiio & Ruedi Aebersold

Published online: 27 June 2006 | doi:10.1038/nprot.2006.22

Abstract - | Full Text - Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry | PDF (184 KB) - Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry

See also: Protocol by Asara et al. | Protocol by Tanner et al. | Protocol by Burlina et al. | Protocol by Samyn et al.

In-cell NMR for protein-protein interactions (STINT-NMR) - pp146 - 152

David S Burz, Kaushik Dutta, David Cowburn & Alexander Shekhtman

Published online: 27 June 2006 | doi:10.1038/nprot.2006.23

Abstract - | Full Text - In-cell NMR for protein-protein interactions (STINT-NMR) | PDF (307 KB) - In-cell NMR for protein-protein interactions (STINT-NMR)

mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa - pp153 - 161

Kyoung-Hee Choi & Herbert P Schweizer

Published online: 27 June 2006 | doi:10.1038/nprot.2006.24

Abstract - | Full Text - mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa | PDF (235 KB) - mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa

See also: Protocol by Choi et al. | Protocol by Choi & Schweizer

mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 - pp162 - 169

Kyoung-Hee Choi, David DeShazer & Herbert P Schweizer

Published online: 27 June 2006 | doi:10.1038/nprot.2006.25

Abstract - | Full Text - mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 | PDF (215 KB) - mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344

See also: Protocol by Choi & Schweizer | Protocol by Choi & Schweizer

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320 - pp170 - 178

Kyoung-Hee Choi & Herbert P Schweizer

Published online: 27 June 2006 | doi:10.1038/nprot.2006.26

Abstract - | Full Text - mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320 | PDF (209 KB) - mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320

See also: Protocol by Choi & Schweizer | Protocol by Choi et al.

Protocol for the fast chromatin immunoprecipitation (ChIP) method - pp179 - 185

Joel D Nelson, Oleg Denisenko & Karol Bomsztyk

Published online: 27 June 2006 | doi:10.1038/nprot.2006.27

Abstract - | Full Text - Protocol for the fast chromatin immunoprecipitation (ChIP) method | PDF (208 KB) - Protocol for the fast chromatin immunoprecipitation (ChIP) method

See also: Protocol by Meng & Wolfe | Protocol by Hallikas & Taipale | Protocol by Palin et al.

Isothermal titration calorimetry to determine association constants for high-affinity ligands - pp186 - 191

Adrian Velazquez-Campoy & Ernesto Freire

Published online: 27 June 2006 | doi:10.1038/nprot.2006.28

Abstract - | Full Text - Isothermal titration calorimetry to determine association constants for high-affinity ligands | PDF (272 KB) - Isothermal titration calorimetry to determine association constants for high-affinity ligands

In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature - pp192 - 199

Christoph Roesli, Dario Neri & Jascha-N Rybak

Published online: 27 June 2006 | doi:10.1038/nprot.2006.29

Abstract - | Full Text - In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature | PDF (209 KB) - In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature

A direct approach to quantification of the cellular uptake of cell-penetrating peptides using MALDI-TOF mass spectrometry - pp200 - 205

Fabienne Burlina, Sandrine Sagan, Gérard Bolbach & Gérard Chassaing

Published online: 27 June 2006 | doi:10.1038/nprot.2006.30

Abstract - | Full Text - A direct approach to quantification of the cellular uptake of cell-penetrating peptides using MALDI-TOF mass spectrometry | PDF (215 KB) - A direct approach to quantification of the cellular uptake of cell-penetrating peptides using MALDI-TOF mass spectrometry

See also: Protocol by Asara et al. | Protocol by Shiio & Aebersold | Protocol by Samyn et al.

Reconstruction of human mammary tissues in a mouse model - pp206 - 214

David A Proia & Charlotte Kuperwasser

Published online: 27 June 2006 | doi:10.1038/nprot.2006.31

Abstract - | Full Text - Reconstruction of human mammary tissues in a mouse model | PDF (548 KB) - Reconstruction of human mammary tissues in a mouse model

High-throughput assay for determining specificity and affinity of protein-DNA binding interactions - pp215 - 222

Outi Hallikas & Jussi Taipale

Published online: 27 June 2006 | doi:10.1038/nprot.2006.33

Abstract - | Full Text - High-throughput assay for determining specificity and affinity of protein-DNA binding interactions | PDF (290 KB) - High-throughput assay for determining specificity and affinity of protein-DNA binding interactions

See also: Protocol by Palin et al. | Protocol by Meng & Wolfe | Protocol by Nelson et al.

One-step analysis of protein complexes in microliters of cell lysate using indirect immunolabeling & fluorescence cross-correlation spectroscopy - pp223 - 229

Oda Stoevesandt & Roland Brock

Published online: 27 June 2006 | doi:10.1038/nprot.2006.34

Abstract - | Full Text - One-step analysis of protein complexes in microliters of cell lysate using indirect immunolabeling & fluorescence cross-correlation spectroscopy | PDF (227 KB) - One-step analysis of protein complexes in microliters of cell lysate using indirect immunolabeling & fluorescence cross-correlation spectroscopy

The counting Stroop: a cognitive interference task - pp230 - 233

George Bush, Paul J Whalen, Lisa M Shin & Scott L Rauch

Published online: 27 June 2006 | doi:10.1038/nprot.2006.35

Abstract - | Full Text - The counting Stroop: a cognitive interference task | PDF (123 KB) - The counting Stroop: a cognitive interference task

See also: Protocol by Whalen et al. | Protocol by Zelazo | Protocol by Bush & Shin

Design and cloning of lentiviral vectors expressing small interfering RNAs - pp234 - 240

Gustavo Tiscornia, Oded Singer & Inder M Verma

Published online: 27 June 2006 | doi:10.1038/nprot.2006.36

Abstract - | Full Text - Design and cloning of lentiviral vectors expressing small interfering RNAs | PDF (365 KB) - Design and cloning of lentiviral vectors expressing small interfering RNAs

See also: Protocol by Tiscornia et al. | Protocol by Singer et al.

Production and purification of lentiviral vectors - pp241 - 245

Gustavo Tiscornia, Oded Singer & Inder M Verma

Published online: 27 June 2006 | doi:10.1038/nprot.2006.37

Abstract - | Full Text - Production and purification of lentiviral vectors | PDF (136 KB) - Production and purification of lentiviral vectors

See also: Protocol by Tiscornia et al. | Protocol by Singer et al.

Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes - pp246 - 252

Juewen Liu & Yi Lu

Published online: 27 June 2006 | doi:10.1038/nprot.2006.38

Abstract - | Full Text - Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes | PDF (342 KB) - Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes

Colony filtration blotting for screening soluble expression in Escherichia coli - pp253 - 258

Sue-Li Dahlroth, Pär Nordlund & Tobias Cornvik

Published online: 27 June 2006 | doi:10.1038/nprot.2006.39

Abstract - | Full Text - Colony filtration blotting for screening soluble expression in Escherichia coli | PDF (334 KB) - Colony filtration blotting for screening soluble expression in Escherichia coli

Rapid functional assays of intracellular Ca²⁺ channels - pp259 - 263

Stephen C Tovey, Yi Sun & Colin W Taylor

Published online: 27 June 2006 | doi:10.1038/nprot.2006.40

Abstract - | Full Text - Rapid functional assays of intracellular Ca2+ channels | PDF (283 KB) - Rapid functional assays of intracellular Ca2+ channels

See also: Protocol by Israelson et al.

COBRA: combined binary ratio labeling of nucleic-acid probes for multi-color fluorescence in situ hybridization karyotyping - pp264 - 275

Karoly Szuhai & Hans J Tanke

Published online: 27 June 2006 | doi:10.1038/nprot.2006.41

Abstract - | Full Text - COBRA: combined binary ratio labeling of nucleic-acid probes for multi-color fluorescence in situ hybridization karyotyping | PDF (414 KB) - COBRA: combined binary ratio labeling of nucleic-acid probes for multi-color fluorescence in situ hybridization karyotyping

The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology - pp276 - 279

Holger Lorenz, Dale W Hailey, Christian Wunder & Jennifer Lippincott-Schwartz

Published online: 27 June 2006 | doi:10.1038/nprot.2006.42

Abstract - | Full Text - The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology | PDF (158 KB) - The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology | Supplementary information

Site-specific protein labeling by Sfp phosphopantetheinyl transferase - pp280 - 285

Jun Yin, Alison J Lin, David E Golan & Christopher T Walsh

Published online: 27 June 2006 | doi:10.1038/nprot.2006.43

Abstract - | Full Text - Site-specific protein labeling by Sfp phosphopantetheinyl transferase | PDF (465 KB) - Site-specific protein labeling by Sfp phosphopantetheinyl transferase

Rapid generation of knockdown transgenic mice by silencing lentiviral vectors - pp286 - 292

Oded Singer, Gustavo Tiscornia, Masahito Ikawa & Inder M Verma

Published online: 27 June 2006 | doi:10.1038/nprot.2006.44

Abstract - | Full Text - Rapid generation of knockdown transgenic mice by silencing lentiviral vectors | PDF (222 KB) - Rapid generation of knockdown transgenic mice by silencing lentiviral vectors

See also: Protocol by Tiscornia et al. | Protocol by Tiscornia et al.

The emotional counting Stroop: a task for assessing emotional interference during brain imaging - pp293 - 296

Paul J Whalen, George Bush, Lisa M Shin & Scott L Rauch

Published online: 27 June 2006 | doi:10.1038/nprot.2006.45

Abstract - | Full Text - The emotional counting Stroop: a task for assessing emotional interference during brain imaging | PDF (126 KB) - The emotional counting Stroop: a task for assessing emotional interference during brain imaging

See also: Protocol by Bush et al. | Protocol by Zelazo | Protocol by Bush & Shin

The Dimensional Change Card Sort (DCCS): a method of assessing executive function in children - pp297 - 301

Philip David Zelazo

Published online: 27 June 2006 | doi:10.1038/nprot.2006.46

Abstract - | Full Text - The Dimensional Change Card Sort (DCCS): a method of assessing executive function in children | PDF (304 KB) - The Dimensional Change Card Sort (DCCS): a method of assessing executive function in children

See also: Protocol by Bush et al. | Protocol by Whalen et al. | Protocol by Bush & Shin

RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts - pp302 - 307

Jack D Keene, Jordan M Komisarow & Matthew B Friedersdorf

Published online: 27 June 2006 | doi:10.1038/nprot.2006.47

Abstract - | Full Text - RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts | PDF (129 KB) - RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts

The Multi-Source Interference Task: an fMRI task that reliably activates the cingulo-frontal-parietal cognitive/attention network - pp308 - 313

George Bush & Lisa M Shin

Published online: 27 June 2006 | doi:10.1038/nprot.2006.48

Abstract - | Full Text - The Multi-Source Interference Task: an fMRI task that reliably activates the cingulo-frontal-parietal cognitive/attention network | PDF (235 KB) - The Multi-Source Interference Task: an fMRI task that reliably activates the cingulo-frontal-parietal cognitive/attention network

See also: Protocol by Bush et al. | Protocol by Whalen et al. | Protocol by Zelazo

Conjugation of chelating agents to proteins and radiolabeling with trivalent metallic isotopes - pp314 - 317

Maggie S Cooper, Emmanuelle Sabbah & Stephen J Mather

Published online: 27 June 2006 | doi:10.1038/nprot.2006.49

Abstract - | Full Text - Conjugation of chelating agents to proteins and radiolabeling with trivalent metallic isotopes | PDF (95 KB) - Conjugation of chelating agents to proteins and radiolabeling with trivalent metallic isotopes

See also: Protocol by Blankenberg et al.

A method for C-terminal sequence analysis in the proteomic era (proteins cleaved with cyanogen bromide) - pp318 - 323

Bart Samyn, Kjell Sergeant & Jozef Van Beeumen

Published online: 27 June 2006 | doi:10.1038/nprot.2006.50

Abstract - | Full Text - A method for C-terminal sequence analysis in the proteomic era (proteins cleaved with cyanogen bromide) | PDF (229 KB) - A method for C-terminal sequence analysis in the proteomic era (proteins cleaved with cyanogen bromide) | Supplementary information

See also: Protocol by Asara et al. | Protocol by Tanner et al. | Protocol by Shiio & Aebersold

The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange - pp324 - 336

Haley D Hill & Chad A Mirkin

Published online: 29 June 2006 | doi:10.1038/nprot.2006.51

Abstract - | Full Text - The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange | PDF (465 KB) - The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange

See also: Protocol by Liu & Lu

Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions - pp337 - 345

Kevin D G Pfleger, Ruth M Seeber & Karin A Eidne

Published online: 27 June 2006 | doi:10.1038/nprot.2006.52

Abstract - | Full Text - Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions | PDF (248 KB) - Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions

High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei - pp346 - 356

Chris J Janse, Jai Ramesar & Andrew P Waters

Published online: 29 June 2006 | doi:10.1038/nprot.2006.53

Abstract - | Full Text - High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei | PDF (560 KB) - High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei | Supplementary information

Physiological 3D tissue model of the airway wall and mucosa - pp357 - 362

Melanie M Choe, Alice A Tomei & Melody A Swartz

Published online: 27 June 2006 | doi:10.1038/nprot.2006.54

Abstract - | Full Text - Physiological 3D tissue model of the airway wall and mucosa | PDF (307 KB) - Physiological 3D tissue model of the airway wall and mucosa | Supplementary information

In vitro measurement of cell death with the annexin A5 affinity assay - pp363 - 367

Hugo van Genderen, Heidi Kenis, Petra Lux, Lisette Ungeth, Cecile Maassen, Niko Deckers, Jagat Narula, Leo Hofstra & Chris Reutelingsperger

Published online: 27 June 2006 | doi:10.1038/nprot.2006.55

Abstract - | Full Text - In vitro measurement of cell death with the annexin A5 affinity assay | PDF (303 KB) - In vitro measurement of cell death with the annexin A5 affinity assay

See also: Protocol by Olive & Banáth | Protocol by Blankenberg et al.

Locating potential enhancer elements by comparative genomics using the EEL software - pp368 - 374

Kimmo Palin, Jussi Taipale & Esko Ukkonen

Published online: 27 June 2006 | doi:10.1038/nprot.2006.56

Abstract - | Full Text - Locating potential enhancer elements by comparative genomics using the EEL software | PDF (671 KB) - Locating potential enhancer elements by comparative genomics using the EEL software

See also: Protocol by Hallikas & Taipale | Protocol by Meng & Wolfe | Protocol by Nelson et al.

Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates - pp375 - 379

Benjamin E. Turk, Jessica E. Hutti & Lewis C. Cantley

Published online: 27 June 2006 | doi:10.1038/nprot.2006.57

Abstract - | Full Text - Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates | PDF (245 KB) - Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates | Supplementary information

Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo - pp380 - 386

Olga Garaschuk, Ruxandra-Iulia Milos & Arthur Konnerth

Published online: 27 June 2006 | doi:10.1038/nprot.2006.58

Abstract - | Full Text - Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo | PDF (1,021 KB) - Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo

Gas chromatography mass spectrometry–based metabolite profiling in plants - pp387 - 396

Jan Lisec, Nicolas Schauer, Joachim Kopka, Lothar Willmitzer & Alisdair R Fernie

Published online: 27 June 2006 | doi:10.1038/nprot.2006.59

Abstract - | Full Text - Gas chromatography mass spectrometry–based metabolite profiling in plants | PDF (605 KB) - Gas chromatography mass spectrometry–based metabolite profiling in plants

Glycome mapping on DNA sequencing equipment - pp397 - 405

Wouter Laroy, Roland Contreras & Nico Callewaert

Published online: 27 June 2006 | doi:10.1038/nprot.2006.60

Abstract - | Full Text - Glycome mapping on DNA sequencing equipment | PDF (598 KB) - Glycome mapping on DNA sequencing equipment

Generation of T-cell receptor retrogenic mice - pp406 - 417

Jeff Holst, Andrea L Szymczak-Workman, Kate M Vignali, Amanda R Burton, Creg J Workman & Dario A A Vignali

Published online: 27 June 2006 | doi:10.1038/nprot.2006.61

Abstract - | Full Text - Generation of T-cell receptor retrogenic mice | PDF (419 KB) - Generation of T-cell receptor retrogenic mice

Blue native PAGE - pp418 - 428

Ilka Wittig, Hans-Peter Braun & Hermann Schägger

Published online: 27 June 2006 | doi:10.1038/nprot.2006.62

Abstract - | Full Text - Blue native PAGE | PDF (255 KB) - Blue native PAGE

See also: Protocol by Schägger

In vivo multimodal imaging of transplanted pancreatic islets - pp429 - 435

Zdravka Medarova, Natalia V Evgenov, Guangping Dai, Susan Bonner-Weir & Anna Moore

Published online: 27 June 2006 | doi:10.1038/nprot.2006.63

Abstract - | Full Text - In vivo multimodal imaging of transplanted pancreatic islets | PDF (285 KB) - In vivo multimodal imaging of transplanted pancreatic islets

Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs - pp436 - 443

Bethany A Janowski, Jiaxin Hu & David R Corey

Published online: 27 June 2006 | doi:10.1038/nprot.2006.64

Abstract - | Full Text - Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs | PDF (268 KB) - Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs

Normal human keratinocyte assay to predict the human irritancy response - pp444 - 451

Richard Clothier & Nancy Khammo

Published online: 27 June 2006 | doi:10.1038/nprot.2006.65

Abstract - | Full Text - Normal human keratinocyte assay to predict the human irritancy response | PDF (179 KB) - Normal human keratinocyte assay to predict the human irritancy response

The 'reverse capture' autoantibody microarray:a native antigen-based platform for autoantibody profiling - pp452 - 460

Joshua R Ehrlich, Shuzhen Qin & Brian C-S Liu

Published online: 27 June 2006 | doi:10.1038/nprot.2006.66

Abstract - | Full Text - The 'reverse capture' autoantibody microarray:a native antigen-based platform for autoantibody profiling | PDF (496 KB) - The 'reverse capture' autoantibody microarray:a native antigen-based platform for autoantibody profiling

Photoconductive stimulation of neurons cultured on silicon wafers - pp461 - 467

Yukiko Goda & Michael A Colicos

Published online: 27 June 2006 | doi:10.1038/nprot.2006.67

Abstract - | Full Text - Photoconductive stimulation of neurons cultured on silicon wafers | PDF (477 KB) - Photoconductive stimulation of neurons cultured on silicon wafers | Supplementary information

Construction of semi-randomized gene libraries with weighted oligonucleotide synthesis and PCR - pp468 - 475

Mark Isalan

Published online: 27 June 2006 | doi:10.1038/nprot.2006.68

Abstract - | Full Text - Construction of semi-randomized gene libraries with weighted oligonucleotide synthesis and PCR | PDF (246 KB) - Construction of semi-randomized gene libraries with weighted oligonucleotide synthesis and PCR | Supplementary information

Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice - pp476 - 485

Blandine Franke-Fayard, Andrew P Waters & Chris J Janse

Published online: 27 June 2006 | doi:10.1038/nprot.2006.69

Abstract - | Full Text - Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice | PDF (762 KB) - Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice

A method for parental RNA interference in the wasp Nasonia vitripennis - pp486 - 494

Jeremy A Lynch & Claude Desplan

Published online: 27 June 2006 | doi:10.1038/nprot.2006.70

Abstract - | Full Text - A method for parental RNA interference in the wasp Nasonia vitripennis | PDF (575 KB) - A method for parental RNA interference in the wasp Nasonia vitripennis