Article abstract
Nature Physics 3, 129 - 134 (2007)
doi:10.1038/nphys514
Subject Categories: Optical physics | Techniques and instrumentation
Interferometric synthetic aperture microscopy
Tyler S. Ralston1,2, Daniel L. Marks1,2, P. Scott Carney1,2 and Stephen A. Boppart1,2,3
Abstract
State-of-the-art methods in high-resolution three-dimensional optical microscopy require that the focus be scanned through the entire region of interest. However, an analysis of the physics of the light–sample interaction reveals that the Fourier-space coverage is independent of depth. Here we show that, by solving the inverse scattering problem for interference microscopy, computed reconstruction yields volumes with a resolution in all planes that is equivalent to the resolution achieved only at the focal plane for conventional high-resolution microscopy. In short, the entire illuminated volume has spatially invariant resolution, thus eliminating the compromise between resolution and depth of field. We describe and demonstrate a novel computational image-formation technique called interferometric synthetic aperture microscopy (ISAM). ISAM has the potential to broadly impact real-time three-dimensional microscopy and analysis in the fields of cell and tumour biology, as well as in clinical diagnosis where in vivo imaging is preferable to biopsy.
- Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA
- Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA
- Departments of Bioengineering, Internal Medicine, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, Illinois 61801, USA
Correspondence to: Stephen A. Boppart1,2,3 e-mail: boppart@uiuc.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
Optical coherence tomography for ultrahigh resolution in vivo imagingNature Biotechnology Research (01 Nov 2003)
Achieving centimetre-scale supercollimation in a large-area two-dimensional photonic crystalNature Materials Letter (01 Feb 2006)
Three-dimensional endomicroscopy using optical coherence tomographyNature Photonics Article (01 Dec 2007)
See all 11 matches for Research
