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Volume 5 Issue 2, February 2010

Single-molecule fluorescence spectroscopy has allowed many chemical and biological systems to be studied both in vitro and in vivo. However, it is difficult to perform such measurements at temperatures above 37â°C because the index-matching fluids used to direct light from the sample to the lens can also conduct heat, and this heating can damage the lens. Now Jerrod Schwartz, Stavros Stavrakis and Stephen Quake have shown that a colloidal titanium dioxide particle can act as a microlens when placed next to an emitting molecule. This microlens focuses light from the molecule into a conventional lens that is separated from the sample by air, which allows single-molecule measurements to be performed on biological systems in real time at temperatures as high as 70â°C. This image is based on a simulation showing how light from a point source is focused by the microlens.

Cover design by Karen Moore

Letter p127; News & Views p99

Editorial

  • The food industry will only reap the benefits of nanotechnology if issues related to safety are addressed and companies are more open about what they are doing.

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News & Views

  • Placing colloidal spheres in the immediate proximity of fluorescent molecules makes it possible to achieve single-molecule imaging at high temperatures with a low-cost system.

    • Yuval Ebenstein
    • Laurent A. Bentolila
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  • The presence of just one dopant atom can dramatically alter the performance of a short-channel transistor, depending on where it is located.

    • Sven Rogge
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  • An atomic force microscope can reveal a range of subsurface information about a sample through mechanical excitation of both the sample and the tip.

    • Ricardo Garcia
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  • Could carbon nanotubes of a single chirality be grown from the bottom up using a common organic reaction?

    • Graham J. Bodwell
    News & Views
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