Letter abstract
Nature Nanotechnology 2, 560 - 564 (2007)
Published online: 2 September 2007 | doi:10.1038/nnano.2007.261
Subject Category: Nanosensors and other devices
Reversible fluorescence quenching in carbon nanotubes for biomolecular sensing
B. C. Satishkumar1, Leif O. Brown1, Yuan Gao1, Chun-Chih Wang1, Hsing-Lin Wang1 & Stephen K. Doorn1
Abstract
Biosensing applications of single-walled carbon nanotubes have been demonstrated in solid-state device structures1, 2, 3. Bioanalyte sensing schemes based on coupling of reversible nanotube fluorescence quenching to redox reactions paired to enzymatic peroxide generation have also been pursued4, 5. Here we show a new approach to highly sensitive nanotube-based optical sensing. Single-walled carbon nanotubes interacting with dye–ligand conjugates—a redox-active dye molecule that is covalently bound to a biological receptor ligand (such as biotin in this case)—showed fluorescence quenching. Further interaction between the receptor ligand on the conjugates and target analytes (avidin in this case) induced the recovery of the quenched fluorescence, forming the basis of the sensing scheme. Nanomolar sensitivity was attained with high specificity for the target analyte. This is a versatile approach because a wide range of conjugation possibilities exists between the potential receptors and redox quenchers.
- Chemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Correspondence to: Stephen K. Doorn1 e-mail: skdoorn@lanl.gov
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
Chiral selectivity in the charge-transfer bleaching of single-walled carbon-nanotube spectraNature Materials Article (01 May 2005)
Near-infrared optical sensors based on single-walled carbon nanotubesNature Materials Article (01 Jan 2005)
Selection of carbon nanotubes with specific chiralities using helical assemblies of flavin mononucleotideNature Nanotechnology Article (01 Jun 2008)
