Articles

  • Article |

    APOBEC3G is shown to induce a potent, non-site-specific interference with reverse transcription through direct interaction with HIV-1 reverse transcriptase, and host DNA repair machinery is shown to cleave HIV-1 cDNA.

    • Darja Pollpeter
    • , Maddy Parsons
    • , Andrew E. Sobala
    • , Sashika Coxhead
    • , Rupert D. Lang
    • , Annie M. Bruns
    • , Stelios Papaioannou
    • , James M. McDonnell
    • , Luis Apolonia
    • , Jamil A. Chowdhury
    • , Curt M. Horvath
    •  & Michael H. Malim
  • Article |

    A machine-learning approach accounting for methodological differences in studies and complex interactions among taxa allows independent soil studies to be combined at the taxonomy-based level to assess bacterial community structure.

    • Kelly S. Ramirez
    • , Christopher G. Knight
    • , Mattias de Hollander
    • , Francis Q. Brearley
    • , Bede Constantinides
    • , Anne Cotton
    • , Si Creer
    • , Thomas W. Crowther
    • , John Davison
    • , Manuel Delgado-Baquerizo
    • , Ellen Dorrepaal
    • , David R. Elliott
    • , Graeme Fox
    • , Robert I. Griffiths
    • , Chris Hale
    • , Kyle Hartman
    • , Ashley Houlden
    • , David L. Jones
    • , Eveline J. Krab
    • , Fernando T. Maestre
    • , Krista L. McGuire
    • , Sylvain Monteux
    • , Caroline H. Orr
    • , Wim H. van der Putten
    • , Ian S. Roberts
    • , David A. Robinson
    • , Jennifer D. Rocca
    • , Jennifer Rowntree
    • , Klaus Schlaeppi
    • , Matthew Shepherd
    • , Brajesh K. Singh
    • , Angela L. Straathof
    • , Jennifer M. Bhatnagar
    • , Cécile Thion
    • , Marcel G. A. van der Heijden
    •  & Franciska T. de Vries
  • Article |

    How the oral epithelium discriminates pathogens from commensals is unclear. Ephrin A2 is now shown to bind exposed β-glucans on the surface of the fungal pathogen Candida albicans, which is required to mount a proinflammatory and antifungal response.

    • Marc Swidergall
    • , Norma V. Solis
    • , Michail S. Lionakis
    •  & Scott G. Filler
  • Article | | open

    Cultivation of a cellulolytic consortium reveals successional community dynamics and the presence of multidomain glycoside hydrolases assembled into stable complexes distinct from cellulosomes, which are produced by a potential pioneer population.

    • Sebastian Kolinko
    • , Yu-Wei Wu
    • , Firehiwot Tachea
    • , Evelyn Denzel
    • , Jennifer Hiras
    • , Raphael Gabriel
    • , Nora Bäcker
    • , Leanne Jade G. Chan
    • , Stephanie A. Eichorst
    • , Dario Frey
    • , Qiushi Chen
    • , Parastoo Azadi
    • , Paul D. Adams
    • , Todd R. Pray
    • , Deepti Tanjore
    • , Christopher J. Petzold
    • , John M. Gladden
    • , Blake A. Simmons
    •  & Steven W. Singer
  • Article |

    This study reports the viral and cellular N 6-methyladenosine (m6A) and N 6,2′-O-dimethyladenosine (m6Am) epitranscriptomes during KSHV latent and lytic infection, and shows that lytic replication induces dynamic epitranscriptome reprogramming of host pathways that control this process.

    • Brandon Tan
    • , Hui Liu
    • , Songyao Zhang
    • , Suzane Ramos da Silva
    • , Lin Zhang
    • , Jia Meng
    • , Xiaodong Cui
    • , Hongfeng Yuan
    • , Océane Sorel
    • , Shao-Wu Zhang
    • , Yufei Huang
    •  & Shou-Jiang Gao
  • Article |

    Adaptation of the polony method allows numerical abundances of diverse viral groups to be quantified in environmental samples, and reveals that clade B T7-like cyanophages that carry the <Emphasis Type=”Italic”>psbA</Emphasis> gene are more abundant in the Red Sea than clade A phages.

    • Nava Baran
    • , Svetlana Goldin
    • , Ilia Maidanik
    •  & Debbie Lindell
  • Article |

    A CRISPR–Cas9-based gene drive array platform is developed and combined with mating-competent Candida albicans haploids to generate homozygous double-deletion mutants, transforming our ability to do genetic interaction analyses in fungi.

    • Rebecca S. Shapiro
    • , Alejandro Chavez
    • , Caroline B. M. Porter
    • , Meagan Hamblin
    • , Christian S. Kaas
    • , James E. DiCarlo
    • , Guisheng Zeng
    • , Xiaoli Xu
    • , Alexey V. Revtovich
    • , Natalia V. Kirienko
    • , Yue Wang
    • , George M. Church
    •  & James J. Collins
  • Article |

    Bacillus subtilis cells are able to sense self-produced autoinducers, which gives rise to stronger quorum-sensing-mediated responses, in a process that can influence the generation of persisters during antibiotic treatment.

    • Tasneem Bareia
    • , Shaul Pollak
    •  & Avigdor Eldar
  • Article |

    Comparative genomics and high-resolution mass spectrometry reveal the biosynthetic gene clusters, and vast chemical diversity of natural products that they encode, in Photorhabdus and Xenorhabdus, bacteria that interact with hosts as symbionts and entomopathogens.

    • Nicholas J. Tobias
    • , Hendrik Wolff
    • , Bardya Djahanschiri
    • , Florian Grundmann
    • , Max Kronenwerth
    • , Yi-Ming Shi
    • , Svenja Simonyi
    • , Peter Grün
    • , David Shapiro-Ilan
    • , Sacha J. Pidot
    • , Timothy P. Stinear
    • , Ingo Ebersberger
    •  & Helge B. Bode
  • Article |

    Under nutritional limitation, modification of the Lactobacillus plantarum cell wall by d-alanylation of teichoic acids is important for host intestinal peptidase expression and consequently growth of the Drosophila host, providing further insights into host–commensal interactions.

    • Renata C. Matos
    • , Martin Schwarzer
    • , Hugo Gervais
    • , Pascal Courtin
    • , Pauline Joncour
    • , Benjamin Gillet
    • , Dali Ma
    • , Anne-Laure Bulteau
    • , Maria Elena Martino
    • , Sandrine Hughes
    • , Marie-Pierre Chapot-Chartier
    •  & François Leulier
  • Article |

    An atomic model for a type 2 secretion system pseudopilus from Klebsiella oxytoca reveals a comprehensive network of inter-subunit contacts while mutational and functional analyses highlight the role of calcium in PulG folding and stability.

    • Aracelys López-Castilla
    • , Jenny-Lee Thomassin
    • , Benjamin Bardiaux
    • , Weili Zheng
    • , Mangayarkarasi Nivaskumar
    • , Xiong Yu
    • , Michael Nilges
    • , Edward H. Egelman
    • , Nadia Izadi-Pruneyre
    •  & Olivera Francetic
  • Article |

    Fluorescent d-amino acid incorporation reveals host–prey cell wall modification during Bdellovibrio bacteriovorus predation including pore formation and l,d-transpeptidase-mediated prey strengthening.

    • Erkin Kuru
    • , Carey Lambert
    • , Jonathan Rittichier
    • , Rob Till
    • , Adrien Ducret
    • , Adeline Derouaux
    • , Joe Gray
    • , Jacob Biboy
    • , Waldemar Vollmer
    • , Michael VanNieuwenhze
    • , Yves V. Brun
    •  & R. Elizabeth Sockett
  • Article |

    Levulinic acid (LA) is a value-added chemical easily obtained from biomass. The pathway enabling LA degradation in Pseudomonas putida requires five enzymes and can be engineered into Escherichia coli, which could enable further biotechnological applications.

    • Jacqueline M. Rand
    • , Tippapha Pisithkul
    • , Ryan L. Clark
    • , Joshua M. Thiede
    • , Christopher R. Mehrer
    • , Daniel E. Agnew
    • , Candace E. Campbell
    • , Andrew L. Markley
    • , Morgan N. Price
    • , Jayashree Ray
    • , Kelly M. Wetmore
    • , Yumi Suh
    • , Adam P. Arkin
    • , Adam M. Deutschbauer
    • , Daniel Amador-Noguez
    •  & Brian F. Pfleger
  • Article | | open

    The recovery of 7,903 bacterial and archaeal metagenome-assembled genomes increases the phylogenetic diversity represented by public genome repositories and provides the first representatives from 20 candidate phyla.

    • Donovan H. Parks
    • , Christian Rinke
    • , Maria Chuvochina
    • , Pierre-Alain Chaumeil
    • , Ben J. Woodcroft
    • , Paul N. Evans
    • , Philip Hugenholtz
    •  & Gene W. Tyson
  • Article |

    The structures of a variant surface glycoprotein (VSG) from Trypanosoma brucei suggest that VSGs adopt different conformations to respond to obstacles present in the cell membrane, enabling them to maintain a protective coat at all times.

    • Thomas Bartossek
    • , Nicola G. Jones
    • , Christin Schäfer
    • , Mislav Cvitković
    • , Marius Glogger
    • , Helen R. Mott
    • , Jochen Kuper
    • , Martha Brennich
    • , Mark Carrington
    • , Ana-Sunčana Smith
    • , Susanne Fenz
    • , Caroline Kisker
    •  & Markus Engstler
  • Article |

    CD81 is shown to interact with SAMHD1 and lead to its proteasomal degradation, thereby impacting dNTP availability and enhancing HIV-1 reverse transcription in primary human T cells.

    • Vera Rocha-Perugini
    • , Henar Suárez
    • , Susana Álvarez
    • , Soraya López-Martín
    • , Gina M. Lenzi
    • , Felipe Vences-Catalán
    • , Shoshana Levy
    • , Baek Kim
    • , María A. Muñoz-Fernández
    • , Francisco Sánchez-Madrid
    •  & Maria Yáñez-Mó
  • Article |

    TRIM23 is identified as an essential regulator of virus-induced autophagy that mediates restriction to several RNA and DNA viruses. K27-mediated ubiquitylation activates TRIM23 GTPase activity, triggering its relocalization and selective autophagy.

    • Konstantin M. J. Sparrer
    • , Sebastian Gableske
    • , Matthew A. Zurenski
    • , Zachary M. Parker
    • , Florian Full
    • , Gavin J. Baumgart
    • , Jiro Kato
    • , Gustavo Pacheco-Rodriguez
    • , Chengyu Liang
    • , Owen Pornillos
    • , Joel Moss
    • , Martha Vaughan
    •  & Michaela U. Gack
  • Article |

    Chikungunya virus infection leads to painful arthritis-like joint inflammation. This study shows that the NLRP3 inflammasome is crucial for alphavirus-induced inflammation and its inhibition is an effective therapeutic strategy.

    • Weiqiang Chen
    • , Suan-Sin Foo
    • , Ali Zaid
    • , Terk-Shin Teng
    • , Lara J. Herrero
    • , Stefan Wolf
    • , Kothila Tharmarajah
    • , Luan D. Vu
    • , Caryn van Vreden
    • , Adam Taylor
    • , Joseph R. Freitas
    • , Rachel W. Li
    • , Trent M. Woodruff
    • , Richard Gordon
    • , David M. Ojcius
    • , Helder I. Nakaya
    • , Thirumala-Devi Kanneganti
    • , Luke A. J. O’Neill
    • , Avril A. B. Robertson
    • , Nicholas J. King
    • , Andreas Suhrbier
    • , Matthew A. Cooper
    • , Lisa F. P. Ng
    •  & Suresh Mahalingam
  • Article |

    This study reports the identification of broadly protective antibodies targeting the influenza B neuraminidase away from its active site. One dose of antibody therapy was more protective in mice than multiple doses of the current standard of care.

    • Teddy John Wohlbold
    • , Kira A. Podolsky
    • , Veronika Chromikova
    • , Ericka Kirkpatrick
    • , Veronica Falconieri
    • , Philip Meade
    • , Fatima Amanat
    • , Jessica Tan
    • , Benjamin R. tenOever
    • , Gene S. Tan
    • , Sriram Subramaniam
    • , Peter Palese
    •  & Florian Krammer
  • Article |

    Both African and epidemic strains of Zika virus are shown to target CD14+ monocytes, which are more susceptible in pregnant women, but African strains are associated with inflammatory responses, and epidemic strains with immunotolerance.

    • Suan-Sin Foo
    • , Weiqiang Chen
    • , Yen Chan
    • , James W. Bowman
    • , Lin-Chun Chang
    • , Younho Choi
    • , Ji Seung Yoo
    • , Jianning Ge
    • , Genhong Cheng
    • , Alexandre Bonnin
    • , Karin Nielsen-Saines
    • , Patrícia Brasil
    •  & Jae U. Jung
  • Article |

    A new class of drugs, hexahydroquinolines, inhibits host haemoglobin endocytosis by Plasmodium falciparum and displays both therapeutic and transmission-blocking activities.

    • Manu Vanaerschot
    • , Leonardo Lucantoni
    • , Tao Li
    • , Jill M. Combrinck
    • , Andrea Ruecker
    • , T. R. Santha Kumar
    • , Kelly Rubiano
    • , Pedro E. Ferreira
    • , Giulia Siciliano
    • , Sonia Gulati
    • , Philipp P. Henrich
    • , Caroline L. Ng
    • , James M. Murithi
    • , Victoria C. Corey
    • , Sandra Duffy
    • , Ori J. Lieberman
    • , M. Isabel Veiga
    • , Robert E. Sinden
    • , Pietro Alano
    • , Michael J. Delves
    • , Kim Lee Sim
    • , Elizabeth A. Winzeler
    • , Timothy J. Egan
    • , Stephen L. Hoffman
    • , Vicky M. Avery
    •  & David A. Fidock