Articles

  • Article |

    Structural analysis of the type VI secretion TssK baseplate protein shows structural similarities with phage receptor-binding protein.

    • Van Son Nguyen
    • , Laureen Logger
    • , Silvia Spinelli
    • , Pierre Legrand
    • , Thi Thanh Huyen Pham
    • , Thi Trang Nhung Trinh
    • , Yassine Cherrak
    • , Abdelrahim Zoued
    • , Aline Desmyter
    • , Eric Durand
    • , Alain Roussel
    • , Christine Kellenberger
    • , Eric Cascales
    •  & Christian Cambillau
  • Article |

    Cycloclasticus bacterial symbionts of mussels and sponges that live in deep-sea gas and oil seeps are capable of using short-chain alkanes as their primary energy source, providing further insight into chemosynthetic symbioses.

    • Maxim Rubin-Blum
    • , Chakkiath Paul Antony
    • , Christian Borowski
    • , Lizbeth Sayavedra
    • , Thomas Pape
    • , Heiko Sahling
    • , Gerhard Bohrmann
    • , Manuel Kleiner
    • , Molly C. Redmond
    • , David L. Valentine
    •  & Nicole Dubilier
  • Article |

    Sequence-specific interactions between the Hepatitis B virus pre-genome and the core protein define the nucleocapsid assembly pathway and trigger virus-like particle formation. Cp-RNA contacts may regulate pre-genome organization, facilitating reverse transcription.

    • Nikesh Patel
    • , Simon J. White
    • , Rebecca F. Thompson
    • , Richard Bingham
    • , Eva U. Weiß
    • , Daniel P. Maskell
    • , Adam Zlotnick
    • , Eric C. Dykeman
    • , Roman Tuma
    • , Reidun Twarock
    • , Neil A. Ranson
    •  & Peter G. Stockley
  • Article |

    Virus attenuation is used to obtain vaccine strains. Here, the rapid evolution of RNA viruses is exploited by engineering their genomes to encode sites that are a mutation away from a stop codon, a clever method to generate attenuated viruses.

    • Gonzalo Moratorio
    • , Rasmus Henningsson
    • , Cyril Barbezange
    • , Lucia Carrau
    • , Antonio V. Bordería
    • , Hervé Blanc
    • , Stephanie Beaucourt
    • , Enzo Z. Poirier
    • , Thomas Vallet
    • , Jeremy Boussier
    • , Bryan C. Mounce
    • , Magnus Fontes
    •  & Marco Vignuzzi
  • Article |

    Methanonatronarchaeia are a distinct class-level lineage of extremely halophilic methanogens, which lack features of classical methanogenesis and have a high intracellular concentration of potassium, suggesting potassium-based osmoprotection.

    • Dimitry Y. Sorokin
    • , Kira S. Makarova
    • , Ben Abbas
    • , Manuel Ferrer
    • , Peter N. Golyshin
    • , Erwin A. Galinski
    • , Sergio Ciordia
    • , María Carmen Mena
    • , Alexander Y. Merkel
    • , Yuri I. Wolf
    • , Mark C. M. van Loosdrecht
    •  & Eugene V. Koonin
  • Article |

    Nitric oxide deficiency promotes tuberculosis through increased neutrophilic inflammation.

    • Bibhuti B. Mishra
    • , Rustin R. Lovewell
    • , Andrew J. Olive
    • , Guoliang Zhang
    • , Wenfei Wang
    • , Eliseo Eugenin
    • , Clare M. Smith
    • , Jia Yao Phuah
    • , Jarukit E. Long
    • , Michelle L. Dubuke
    • , Samantha G. Palace
    • , Jon D. Goguen
    • , Richard E. Baker
    • , Subhalaxmi Nambi
    • , Rabinarayan Mishra
    • , Matthew G. Booty
    • , Christina E. Baer
    • , Scott A. Shaffer
    • , Veronique Dartois
    • , Beth A. McCormick
    • , Xinchun Chen
    •  & Christopher M. Sassetti
  • Article |

    Cryo-electron microscopy of the tripartite MacA–MacB–TolC multidrug transporter.

    • Anthony W. P. Fitzpatrick
    • , Salomé Llabrés
    • , Arthur Neuberger
    • , James N. Blaza
    • , Xiao-Chen Bai
    • , Ui Okada
    • , Satoshi Murakami
    • , Hendrik W. van Veen
    • , Ulrich Zachariae
    • , Sjors H. W. Scheres
    • , Ben F. Luisi
    •  & Dijun Du
  • Article |

    Metabolomics analyses of Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae show that, unlike other metabolites, coenzymes such as pyridoxal 5'-phosphate, NAD(P), coenzyme A and flavins are long-lived in vivo and passed on over generations.

    • Johannes Hartl
    • , Patrick Kiefer
    • , Fabian Meyer
    •  & Julia A. Vorholt
  • Article |

    Plasmodium falciparum PTEF is a translation-enhancing factor that interacts with ribosomes to facilitate translation of PfEMP1–VAR2CSA, a ligand that mediates adhesion of infected red blood cells to the placenta during pregnancy-associated malaria.

    • Sherwin Chan
    • , Alejandra Frasch
    • , Chandra Sekhar Mandava
    • , Jun-Hong Ch'ng
    • , Maria del Pilar Quintana
    • , Mattias Vesterlund
    • , Mehdi Ghorbal
    • , Nicolas Joannin
    • , Oscar Franzén
    • , Jose-Juan Lopez-Rubio
    • , Sonia Barbieri
    • , Antonio Lanzavecchia
    • , Suparna Sanyal
    •  & Mats Wahlgren
  • Article |

    Deubiquitinase OTULIN targets linear (M1-linked) ubiquitin chain patches on cytosolic Salmonella Typhimurium to modulate NEMO, IKKα/IKKβ and NF-ĸB signalling and regulate secretion of pro-inflammatory cytokines and bacterial proliferation.

    • Sjoerd J. L. van Wijk
    • , Franziska Fricke
    • , Lina Herhaus
    • , Jalaj Gupta
    • , Katharina Hötte
    • , Francesco Pampaloni
    • , Paolo Grumati
    • , Manuel Kaulich
    • , Yu-shin Sou
    • , Masaaki Komatsu
    • , Florian R. Greten
    • , Simone Fulda
    • , Mike Heilemann
    •  & Ivan Dikic
  • Article |

    The use of mitomycin C inductions to determine the fraction of lysogenic cells in mixed natural communities is highly variable and insensitive to bacterial host density, suggesting that other methods should be developed and used to measure lysogeny.

    • Ben Knowles
    • , Barbara Bailey
    • , Lance Boling
    • , Mya Breitbart
    • , Ana Cobián-Güemes
    • , Javier del Campo
    • , Rob Edwards
    • , Ben Felts
    • , Juris Grasis
    • , Andreas F. Haas
    • , Parag Katira
    • , Linda Wegley Kelly
    • , Antoni Luque
    • , Jim Nulton
    • , Lauren Paul
    • , Gregory Peters
    • , Nate Robinett
    • , Stuart Sandin
    • , Anca Segall
    • , Cynthia Silveira
    • , Merry Youle
    •  & Forest Rohwer
  • Article |

    Structural characterization sheds mechanistic insights behind λN-dependent processive antitermination.

    • Nelly Said
    • , Ferdinand Krupp
    • , Ekaterina Anedchenko
    • , Karine F. Santos
    • , Olexandr Dybkov
    • , Yong-Heng Huang
    • , Chung-Tien Lee
    • , Bernhard Loll
    • , Elmar Behrmann
    • , Jörg Bürger
    • , Thorsten Mielke
    • , Justus Loerke
    • , Henning Urlaub
    • , Christian M. T. Spahn
    • , Gert Weber
    •  & Markus C. Wahl
  • Article |

    Drosophila Bap180 is induced by immune deficiency/Relish in response to both pathogenic and commensal bacteria. Bap180 can feedback to restrain immune deficiency signalling and repress pro-inflammatory gene eiger (TNF), limiting tissue damage and elongating life span.

    • Xiaomeng He
    • , Junjing Yu
    • , Min Wang
    • , Yang Cheng
    • , Yanan Han
    • , Shuo Yang
    • , Guizhi Shi
    • , Lei Sun
    • , Ying Fang
    • , Si-tang Gong
    • , Zhong Wang
    • , Yang-Xin Fu
    • , Lei Pan
    •  & Hong Tang
  • Article |

    Comparative genomics of 24 Penicillium species, including 9 that are newly sequenced, characterizes over 1000 secondary metabolism gene clusters, some of which are validated experimentally, identifying these fungi as an important untapped source of bioactive compounds.

    • Jens Christian Nielsen
    • , Sietske Grijseels
    • , Sylvain Prigent
    • , Boyang Ji
    • , Jacques Dainat
    • , Kristian Fog Nielsen
    • , Jens Christian Frisvad
    • , Mhairi Workman
    •  & Jens Nielsen
  • Article |

    This study shows that mitochondrial DNA leaks into the cytoplasm during dengue virus infection, activating the DNA sensor cGAS. Viral NS2B targets cGAS for lysosomal degradation, inhibiting type I interferon responses in infected cells.

    • Sebastian Aguirre
    • , Priya Luthra
    • , Maria T. Sanchez-Aparicio
    • , Ana M. Maestre
    • , Jenish Patel
    • , Francise Lamothe
    • , Anthony C. Fredericks
    • , Shashank Tripathi
    • , Tongtong Zhu
    • , Jessica Pintado-Silva
    • , Laurence G. Webb
    • , Dabeiba Bernal-Rubio
    • , Alexander Solovyov
    • , Benjamin Greenbaum
    • , Viviana Simon
    • , Christopher F. Basler
    • , Lubbertus C. F. Mulder
    • , Adolfo García-Sastre
    •  & Ana Fernandez-Sesma
  • Article |

    In addition to canonical guide-dependent endonuclease activity, the Argonaute protein from the archaeon Methanocaldococcus jannaschii (MjAgo) is capable of guide-independent DNA cleavage, enabling MjAgo to process plasmids and genomic DNA.

    • Adrian Zander
    • , Sarah Willkomm
    • , Sapir Ofer
    • , Marleen van Wolferen
    • , Luisa Egert
    • , Sabine Buchmeier
    • , Sarah Stöckl
    • , Philip Tinnefeld
    • , Sabine Schneider
    • , Andreas Klingl
    • , Sonja-Verena Albers
    • , Finn Werner
    •  & Dina Grohmann
  • Article |

    Trypanosoma brucei, which is responsible for human sleeping sickness, has an intrinsic circadian clock that regulates metabolism and influences drug sensitivity.

    • Filipa Rijo-Ferreira
    • , Daniel Pinto-Neves
    • , Nuno L. Barbosa-Morais
    • , Joseph S. Takahashi
    •  & Luisa M. Figueiredo
  • Article |

    The Plasmodium falciparum telomere repeat-binding zinc finger protein (PfTRZ) binds telomeres, subtelomeric var genes and 5S ribosomal DNA genes. PfTRZ regulates multiple processes in the parasite, including telomere length homeostasis and cell cycle progression.

    • Nicole L. Bertschi
    • , Christa G. Toenhake
    • , Angela Zou
    • , Igor Niederwieser
    • , Rob Henderson
    • , Suzette Moes
    • , Paul Jenoe
    • , John Parkinson
    • , Richard Bartfai
    •  & Till S. Voss
  • Article |

    Cryo-EM reveals one mechanism of action of the antimalarial mefloquine: mefloquine binds to the Plasmodium falciparum 80S ribosome, inhibiting protein synthesis in the parasite.

    • Wilson Wong
    • , Xiao-Chen Bai
    • , Brad E. Sleebs
    • , Tony Triglia
    • , Alan Brown
    • , Jennifer K. Thompson
    • , Katherine E. Jackson
    • , Eric Hanssen
    • , Danushka S. Marapana
    • , Israel S. Fernandez
    • , Stuart A. Ralph
    • , Alan F. Cowman
    • , Sjors H. W. Scheres
    •  & Jake Baum