A llama. Llamas produce single chain antibodies that are used to generate small antigen-binding proteins known as nanobodies. Original image by Capralogics, Inc. Article p1253

Image of a rotifer showing the mouth interior and heart-shaped corona, photographed using differential interference contrast microscopy (40× magnification) by Rogelio Moreno, Panama City, Panama. Winner of the 2014 Nikon Small World photomicrography contest (reprinted with permission from Nikon).

The cover celebrates ten years of Nature Methods. Design by Erin Dewalt, based on images of the number '10' generated by multiple methods, contributed by Yonggang Ke (Georgia Institute of Technology and Emory University); Kristina Woodruff and Sebastian Maerkl (Ecole Polytechnique Fédérale de Lausanne); Akira Takai, Yasushi Okada, Masahiro Nakano and Takeharu Nagai (Osaka University); Alan Shaw and Björn Högberg (Karolinska Institutet); Lauren Polstein and Charles Gersbach (Duke University); Sandra Duffy (Griffith University); and Navneet Dogra and T. Kyle Vanderlick (Yale University). Visit Methagora for more information about these images.

Vector drawings indicating the direction of movement of nuclei in a fruit fly embryo imaged with a light-sheet microscope. Image provided by Kristin Branson, Fernando Amat, William Lemon and Philipp Keller (Janelia Farm Research Campus, Howard Hughes Medical Institute). Article p951

DNA origami–based 'nanocalipers' present ligands at well-defined positions to enable probing of the spatial regulation of membrane receptor function. Artwork by Björn Högberg. Article p841

An artistic depiction of wholebrain imaging in larval zebrafish with lightfield microscopy. Cover design by The Research Institute of Molecular Pathology (IMP). Brief Communication p727

On the cover: Wired and wireless multielectrode neurophysiological recordings in freely moving rhesus monkeys. Cover design by Katie Zhuang. Article p670

On the cover: The Focus on Synthetic Biology takes readers from DNA synthesis to gene assembly, circuit design and whole-genome assembly. Artistic rendition by Erin Dewalt.

On the cover: Highly multiplexed analysis of human breast cancer tissue by imaging mass cytometry at 1-micrometer resolution. Image by Nicole Seidel, Graphic Design Seidel & Risse. Article p417

The cover shows a sample of single-particle image data overlaid with tracking result trajectories from a competition to assess the performance of single-particle tracking algorithms. Image by Erik Meijering. Analysis p281

Measuring force exerted on an oil microdroplet within living embryonic mouse tissue. Cover by Erin Dewalt, based on an image provided by Otger Campàs. Article p183

Single-cell sequencing, our choice for Method of the Year 2013, can be used to decipher the entire genome or transcriptome of an individual cell. Cover design by Erin Dewalt. Special feature begins on p13.