Research

This list contains the 50 most recently published research articles, including advance online publication articles that have not yet been published in a journal issue.

Showing: 1–25 of 50

AOP
  1. Addressing preferred specimen orientation in single-particle cryo-EM through tilting AOP

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    The preferred specimen orientation problem limits accuracy and resolution in structure determination by cryo-EM. Collecting data at defined sample tilts yielded near-atomic-resolution structures for the influenza hemagglutinin trimer and ribosomal biogenesis intermediates.

  2. Video rate volumetric Ca2+ imaging across cortex using seeded iterative demixing (SID) microscopy AOP

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    The seeded iterative demixing strategy, when used in combination with light-field microscopy, enables calcium imaging at single-neuron resolution in the mouse brain at high volumetric imaging rates and depths of up to 380 μm.

  3. CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping AOP

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    CrY2H-seq, a Cre recombinase reporter-mediated yeast two-hybrid method coupled with next-generation sequencing, enables ultra-high-throughput screening of transcription factor interactions in Arabidopsis thaliana.

  4. Adaptive optics improves multiphoton super-resolution imaging AOP

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    Adaptive optics and two-photon instant structured illumination microscopy are combined to provide improved super-resolution imaging within optically aberrating biological samples.

  5. High-speed fixed-target serial virus crystallography AOP

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    A new sample-delivery method for serial X-ray crystallography exploits the full repetition rate of the X-ray free-electron laser at the LCLS facility, thus enabling efficient, high-speed data collection to solve the three-dimensional structures of viruses.

  6. Image Data Resource: a bioimage data integration and publication platform AOP

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    This Resource describes the Image Data Resource (IDR), a prototype online system for biological image data that links experimental and analytic data across multiple data sets and promotes image data sharing and reanalysis.

  7. webKnossos: efficient online 3D data annotation for connectomics

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    webKnossos is a browser-based tracing and annotation tool for 3D electron microscopy data sets that is optimized for seamless data viewing. The tool’s flight-mode view facilitates fast neurite tracing because of its egocentric viewpoint.

  8. Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism

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    Genetically encoded iNap sensors allow imaging of NADPH with high spatiotemporal resolution in living systems. The iNaps cover physiologically relevant NADPH concentrations and are demonstrated in mammalian cells and live zebrafish.

    See also: News and Views by Andreas Wiederkehr et al.

  9. Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice

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    FHIRM-TPM is a miniature two-photon microscope capable of imaging fluorescently labeled neurons in the brains of freely behaving mice. It allows for imaging of spines or recording of neural activity with a frame rate up to 40 Hz.

  10. Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing

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    A new approach to evolve novel aminoacyl-tRNA synthetase–tRNA pairs with orthogonal substrate specificity is applied to generate a system to site-specifically incorporate phosphothreonine into proteins, enabling functional studies of this post-translational modification.

  11. Allele-specific expression reveals interactions between genetic variation and environment

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    The EAGLE algorithm and software identifies replicable gene-by-environment interactions based on associations between environment and allele-specific expression.